##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	530650
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.332006030340146	33.0	31.0	34.0	30.0	34.0
2	32.43199472345237	34.0	31.0	34.0	31.0	34.0
3	32.54040327899746	34.0	31.0	34.0	31.0	34.0
4	36.02551210779233	37.0	35.0	37.0	35.0	37.0
5	35.988468858946575	37.0	35.0	37.0	35.0	37.0
6	36.00692169980213	37.0	35.0	37.0	35.0	37.0
7	36.17429379063413	37.0	36.0	37.0	35.0	37.0
8	36.15930839536418	37.0	36.0	37.0	35.0	37.0
9	37.72765664750777	39.0	38.0	39.0	35.0	39.0
10	37.49361537736738	39.0	37.0	39.0	35.0	39.0
11	37.442977480448505	39.0	37.0	39.0	35.0	39.0
12	37.386527843211155	39.0	37.0	39.0	35.0	39.0
13	37.34816357297654	39.0	37.0	39.0	34.0	39.0
14	38.58908319984924	40.0	38.0	41.0	34.0	41.0
15	38.663650240271366	40.0	38.0	41.0	35.0	41.0
16	38.67162536511825	40.0	38.0	41.0	35.0	41.0
17	38.68866107603882	40.0	38.0	41.0	35.0	41.0
18	38.64127390935645	40.0	38.0	41.0	35.0	41.0
19	38.61675492320739	40.0	38.0	41.0	35.0	41.0
20	38.50827475737303	40.0	38.0	41.0	34.0	41.0
21	38.47604635823989	40.0	38.0	41.0	34.0	41.0
22	38.441705455573356	40.0	38.0	41.0	34.0	41.0
23	38.411207010270424	40.0	38.0	41.0	34.0	41.0
24	38.39890511636672	40.0	37.0	41.0	34.0	41.0
25	38.361920286441155	40.0	37.0	41.0	34.0	41.0
26	38.238074060114954	40.0	37.0	41.0	34.0	41.0
27	38.18810892301894	40.0	37.0	41.0	34.0	41.0
28	38.104556675774994	40.0	37.0	41.0	34.0	41.0
29	38.065608216338454	40.0	37.0	41.0	34.0	41.0
30	38.04202770187506	40.0	37.0	41.0	34.0	41.0
31	37.938219165174786	40.0	37.0	41.0	33.0	41.0
32	37.79988504664091	40.0	37.0	41.0	33.0	41.0
33	37.728832563836804	40.0	36.0	41.0	33.0	41.0
34	37.57463865071139	40.0	36.0	41.0	33.0	41.0
35	37.575795722227454	40.0	36.0	41.0	33.0	41.0
36	37.44362008857062	40.0	36.0	41.0	33.0	41.0
37	37.391868463205505	40.0	36.0	41.0	33.0	41.0
38	37.311955149345145	40.0	36.0	41.0	32.0	41.0
39	37.26097993027419	40.0	35.0	41.0	32.0	41.0
40	37.187038537642515	40.0	35.0	41.0	32.0	41.0
41	37.09474795062659	39.0	35.0	41.0	32.0	41.0
42	37.031764816734196	39.0	35.0	41.0	31.0	41.0
43	36.95847545463111	39.0	35.0	41.0	31.0	41.0
44	36.76914350325073	39.0	35.0	41.0	31.0	41.0
45	36.73248091962687	39.0	35.0	41.0	31.0	41.0
46	36.67074531235277	39.0	35.0	41.0	31.0	41.0
47	36.56651465184208	39.0	35.0	41.0	31.0	41.0
48	36.454546311127864	39.0	35.0	41.0	30.0	41.0
49	36.37347404127014	39.0	35.0	41.0	30.0	41.0
50	36.27813247903514	39.0	35.0	41.0	30.0	41.0
51	35.04323000094224	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	1.0
12	3.0
13	5.0
14	10.0
15	14.0
16	24.0
17	43.0
18	89.0
19	155.0
20	303.0
21	506.0
22	734.0
23	1128.0
24	1758.0
25	2411.0
26	3208.0
27	4105.0
28	4784.0
29	5874.0
30	7319.0
31	9181.0
32	11929.0
33	15844.0
34	27129.0
35	38747.0
36	37170.0
37	54536.0
38	99833.0
39	203633.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28879675869217	20.802223687929896	25.540940356166963	10.368039197210969
2	31.985112597757464	24.39649486478847	26.10496560821634	17.513426929237728
3	32.15019315933289	24.72345236973523	26.02355601620654	17.102798454725338
4	28.298501837369265	26.946198058984265	25.66003957410723	19.095260529539242
5	25.78912654291906	30.98577216621125	24.129840761330442	19.095260529539242
6	27.05059832281165	35.2345236973523	22.671817582210497	15.043060397625554
7	83.63629510977103	5.340619994346556	6.782248186186752	4.240836709695657
8	84.59304626401583	4.78450956374258	6.4656553283708655	4.156788843870725
9	77.22415905022143	7.852256666352586	9.156883067935551	5.7667012154904365
10	36.546311127862054	34.96579666446811	15.124281541505702	13.363610666164139
11	27.1487797983605	24.99462922830491	29.234523697352305	18.622067275982285
12	26.292848393479694	23.57128050504099	30.942052200131915	19.193818901347402
13	23.6617356072741	24.86686139640064	31.966833129181193	19.50456986714407
14	20.198247432394233	28.51276736078394	29.67718835390559	21.611796852916235
15	19.79459153867898	27.57976067087534	33.48836332799397	19.13728446245171
16	22.492603410911148	25.777254310750962	31.78648826910393	19.943654009233956
17	22.573259210402334	25.030622821068498	29.25694902478093	23.139168943748235
18	22.922076698388768	25.639121831715823	29.787053613492887	21.651747856402526
19	23.50212004145859	27.17252426269669	28.14887402242533	21.176481673419392
20	26.144728163572978	26.063695467822484	28.205408461321017	19.58616790728352
21	24.38914538773203	27.02968058042024	28.28662960520117	20.294544426646567
22	22.867049844530293	25.52831433148026	27.50212004145859	24.10251578253086
23	21.621030811269197	27.27014039385659	28.433053801941018	22.675774992933196
24	21.594082728728917	25.68585696786959	30.33637991142938	22.38368039197211
25	21.167059266936775	28.0652030528597	28.193536229152926	22.574201451050598
26	21.198718552718365	28.921322905870163	28.228022236879298	21.651936304532178
27	22.251766701215487	28.558560256289457	28.08480165834354	21.104871384151515
28	20.296994252332045	28.940921511354002	29.239234900593615	21.52284933572034
29	21.725619523226232	27.859417695279376	28.41307830019787	22.001884481296525
30	23.783473099029493	26.520116837840384	28.05314237256195	21.643267690568173
31	23.010647319325354	28.95637425798549	25.4665033449543	22.566475077734854
32	22.77150664279657	30.337510600207292	25.5439555262414	21.347027230754733
33	23.90539903891454	28.360312823895224	25.712239706020917	22.02204843116932
34	21.59935927635918	28.84104400263827	27.46876472251013	22.090831998492416
35	22.542919061528313	28.28512202016395	26.553472156788843	22.61848676151889
36	23.644209931216434	29.463865071139168	26.12098369923678	20.770941298407614
37	22.46753980966739	29.61688495241685	26.90115895599736	21.0144162819184
38	21.519268821256947	29.915575237915764	26.3667200603034	22.19843588052389
39	23.542636389333836	27.138980495618583	26.994629228304913	22.323753886742672
40	23.14689531706398	26.665033449543014	28.49467634033732	21.693394893055686
41	21.2788090078206	27.673042495053235	27.516630547441817	23.53151794968435
42	22.929049279185904	28.305474418166398	26.840478658249317	21.92499764439838
43	23.73786865165363	26.983510788655423	27.413549420522	21.865071139168943
44	22.620559690945065	27.699802129463862	27.048148497126167	22.6314896824649
45	22.38047677376802	26.879487421087344	26.638273815132386	24.10176199001225
46	22.714595307641574	27.519834165645907	26.256477904456798	23.50909262225572
47	21.65702440403279	27.989070008480166	27.90389145387732	22.450014133609724
48	21.77763120701027	26.38386884010176	28.318854235371717	23.519645717516255
49	22.77150664279657	26.052388580043345	28.473758597945913	22.70234617921417
50	21.413549420522003	26.149627814943937	28.17412607179874	24.262696692735325
51	20.619805898426456	25.527183642702344	27.69923678507491	26.153773673796287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	403.5
2	633.0
3	1343.0
4	2053.0
5	1427.0
6	801.0
7	818.0
8	835.0
9	821.0
10	807.0
11	820.5
12	834.0
13	881.5
14	929.0
15	962.5
16	996.0
17	1021.5
18	1047.0
19	1111.0
20	1175.0
21	1390.0
22	1605.0
23	2100.0
24	2595.0
25	2939.5
26	4168.5
27	5053.0
28	6261.0
29	7469.0
30	8414.5
31	9360.0
32	11453.5
33	13547.0
34	15337.0
35	17127.0
36	17986.5
37	18846.0
38	20570.5
39	22295.0
40	25349.5
41	28404.0
42	30581.5
43	32759.0
44	35490.0
45	38221.0
46	42232.5
47	46244.0
48	48971.0
49	51698.0
50	50376.5
51	49055.0
52	43887.0
53	38719.0
54	34592.0
55	30465.0
56	27077.5
57	23690.0
58	21845.0
59	20000.0
60	18878.0
61	17756.0
62	16585.5
63	15415.0
64	12368.0
65	9321.0
66	7585.5
67	5850.0
68	4885.5
69	3921.0
70	3335.5
71	2750.0
72	2249.0
73	1748.0
74	1530.5
75	1096.5
76	880.0
77	690.5
78	501.0
79	328.0
80	155.0
81	166.0
82	177.0
83	132.5
84	88.0
85	57.5
86	27.0
87	17.0
88	7.0
89	11.5
90	16.0
91	9.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	530650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.75141391894479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97756532814238	22.853894696117383
2	9.642778056006438	6.123436747695566
3	3.307125298580773	3.15017712711156
4	1.7624420513511911	2.2384010832242334
5	1.2404900486163506	1.969365649797485
6	0.9696229747762717	1.8472140250463984
7	0.7885705630891624	1.7526761247057554
8	0.7387018858356478	1.8763863471898203
9	0.6986613589346099	1.9965137397034733
>10	8.780537343412686	52.77692136261854
>50	0.07027771189827707	1.4944818929026946
>100	0.02084508403762455	1.2004265539853605
>500	0.0011911476592928315	0.28573480199620027
>1k	0.0011911476592928315	0.43436984790554073
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1218	0.22952982191651747	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1079	0.20333553189484593	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	800	0.15075850372185057	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	711	0.13398662018279467	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8844812965231321E-4	0.0	0.0	0.17092245359464808	0.0
2	1.8844812965231321E-4	0.0	0.0	0.7308018467916706	0.0
3	1.8844812965231321E-4	0.0	0.0	1.0155469706963158	0.0
4	1.8844812965231321E-4	0.0	0.0	1.342316027513427	0.0
5	1.8844812965231321E-4	0.0	0.0	2.3294073306322436	0.0
6	1.8844812965231321E-4	0.0	0.0	2.9876566475077735	0.0
7	1.8844812965231321E-4	0.0	0.0	3.6506171676246115	0.0
8	1.8844812965231321E-4	0.0	0.0	4.832375388674268	0.0
9	1.8844812965231321E-4	0.0	0.0	5.323659662677848	0.0
10	1.8844812965231321E-4	0.0	0.0	6.311504758315274	0.0
11	1.8844812965231321E-4	0.0	0.0	7.510034862903986	0.0
12	1.8844812965231321E-4	0.0	0.0	8.480165834354095	0.0
13	1.8844812965231321E-4	0.0	0.0	8.868745877697163	0.0
14	1.8844812965231321E-4	0.0	0.0	9.032695750494677	0.0
15	1.8844812965231321E-4	0.0	0.0	9.337227928012814	0.0
16	3.7689625930462643E-4	0.0	0.0	9.971921228681806	0.0
17	3.7689625930462643E-4	0.0	0.0	10.7820597380571	0.0
18	3.7689625930462643E-4	0.0	0.0	11.60652030528597	0.0
19	3.7689625930462643E-4	0.0	0.0	12.111184396494865	0.0
20	3.7689625930462643E-4	0.0	0.0	12.588711957033826	0.0
21	3.7689625930462643E-4	0.0	0.0	13.163101856214077	0.0
22	3.7689625930462643E-4	0.0	0.0	13.840384434184491	0.0
23	3.7689625930462643E-4	0.0	0.0	14.487703759540187	0.0
24	3.7689625930462643E-4	0.0	0.0	15.00367473852822	0.0
25	3.7689625930462643E-4	0.0	0.0	15.432582681616886	0.0
26	5.653443889569396E-4	0.0	0.0	15.844907189296146	0.0
27	5.653443889569396E-4	0.0	0.0	16.265900310939415	7.537925186092529E-4
28	5.653443889569396E-4	0.0	0.0	16.716291340808443	7.537925186092529E-4
29	5.653443889569396E-4	0.0	0.0	17.205879581645153	7.537925186092529E-4
30	5.653443889569396E-4	0.0	0.0	17.76199001224913	7.537925186092529E-4
31	7.537925186092529E-4	0.0	0.0	18.234241025157825	7.537925186092529E-4
32	7.537925186092529E-4	0.0	0.0	18.727786676717233	7.537925186092529E-4
33	7.537925186092529E-4	0.0	0.0	19.151229624045982	7.537925186092529E-4
34	7.537925186092529E-4	0.0	0.0	19.591632903043436	7.537925186092529E-4
35	7.537925186092529E-4	0.0	0.0	20.052577028172994	7.537925186092529E-4
36	7.537925186092529E-4	0.0	0.0	20.551776123621973	7.537925186092529E-4
37	7.537925186092529E-4	0.0	0.0	21.00537077169509	7.537925186092529E-4
38	7.537925186092529E-4	0.0	0.0	21.45595024969377	7.537925186092529E-4
39	7.537925186092529E-4	0.0	0.0	21.897861113728446	7.537925186092529E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCG	35	1.2102828E-7	45.000004	1
TGTCGCG	20	7.029849E-4	45.000004	1
TACACGG	35	1.2102828E-7	45.000004	2
GCGTTAG	30	2.163284E-6	44.999996	1
CTAACGG	30	2.163284E-6	44.999996	2
TGTTACG	30	2.163284E-6	44.999996	1
TTGTGCG	55	6.002665E-11	40.909092	1
GCGATAT	90	0.0	40.000004	9
GCGATGC	175	0.0	39.857143	9
GCGAGAC	130	0.0	39.807693	21
GCGCGAC	75	0.0	39.000004	9
TCGTTGA	35	6.241933E-6	38.57143	24
CGAGACA	135	0.0	38.333336	22
CGAATAT	65	9.094947E-12	38.076927	14
CACGTGA	130	0.0	38.076927	43
GTAGGGC	190	0.0	37.894737	4
AGCGTTG	30	1.1390939E-4	37.499996	1
TTAGGCG	30	1.1390939E-4	37.499996	1
CGTTGAT	30	1.1390939E-4	37.499996	25
GTTAGCG	30	1.1390939E-4	37.499996	1
>>END_MODULE