FastQCFastQC Report
Sat 18 Jun 2016
SRR3550474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621488
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100371.6149949797904386No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTT12960.20853178178822437No Hit
CGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTG12210.19646397034214658No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT11390.18326982982776818No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC11340.18246530906469632No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC9340.15028447854182223No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCT9050.14561825811600546No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTC9020.14513554565816236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG302.1638862E-645.0000041
GCCGATA207.030783E-445.09
CCGACGG207.030783E-445.02
TATAGCG253.888731E-545.01
GCTACGA502.1827873E-1145.010
CCGCATG207.030783E-445.01
CGTTTTT53850.043.871871
AAACACG1350.041.66666840
AATACGG556.002665E-1140.909092
ACACGAC1500.040.50000426
CGAATAT501.0804797E-940.514
ATATCGG403.4548066E-739.3752
ACGATAG403.4548066E-739.3751
TCTACGG403.4548066E-739.3752
GCGAGAC1550.039.19354621
GACACGA1550.039.19354625
CACGACC1550.039.19354627
ACGACCA1550.039.19354628
ATCGAGG356.2436593E-638.5714262
AACACGT1400.038.57142641