##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621488
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17639761346961	33.0	31.0	34.0	30.0	34.0
2	32.32146557938367	34.0	31.0	34.0	30.0	34.0
3	32.33612555672837	34.0	31.0	34.0	30.0	34.0
4	35.90034884020287	37.0	35.0	37.0	35.0	37.0
5	35.88435335839147	37.0	35.0	37.0	35.0	37.0
6	35.91414154416497	37.0	35.0	37.0	35.0	37.0
7	36.08159288674922	37.0	35.0	37.0	35.0	37.0
8	36.062657686069564	37.0	35.0	37.0	35.0	37.0
9	37.6766309244909	39.0	38.0	39.0	35.0	39.0
10	37.421396712406356	39.0	37.0	39.0	34.0	39.0
11	37.39959902685168	39.0	37.0	39.0	35.0	39.0
12	37.35354182220735	39.0	37.0	39.0	34.0	39.0
13	37.32719054913369	39.0	37.0	39.0	34.0	39.0
14	38.55938650464691	40.0	38.0	41.0	34.0	41.0
15	38.61148405118039	40.0	38.0	41.0	34.0	41.0
16	38.61100938393018	40.0	38.0	41.0	34.0	41.0
17	38.6326686919136	40.0	38.0	41.0	34.0	41.0
18	38.46830510001802	40.0	38.0	41.0	34.0	41.0
19	38.28816968308318	40.0	37.0	41.0	34.0	41.0
20	38.05008463558428	40.0	36.0	41.0	34.0	41.0
21	37.99785997477023	40.0	36.0	41.0	34.0	41.0
22	37.98422495687769	40.0	36.0	41.0	34.0	41.0
23	37.96659790695878	40.0	36.0	41.0	34.0	41.0
24	37.93019334242978	40.0	35.0	41.0	34.0	41.0
25	37.87433707489123	40.0	35.0	41.0	34.0	41.0
26	37.742620935561106	40.0	35.0	41.0	33.0	41.0
27	37.67728580439204	40.0	35.0	41.0	33.0	41.0
28	37.56461428056535	40.0	35.0	41.0	33.0	41.0
29	37.48160704631465	39.0	35.0	41.0	33.0	41.0
30	37.393561903045594	39.0	35.0	41.0	33.0	41.0
31	37.16494445588652	39.0	35.0	41.0	32.0	41.0
32	36.83892850706691	39.0	35.0	41.0	31.0	41.0
33	36.47863836469892	39.0	35.0	41.0	30.0	41.0
34	36.057687356795306	39.0	35.0	41.0	28.0	41.0
35	35.885344527971576	39.0	35.0	41.0	27.0	41.0
36	35.67786023221687	39.0	35.0	41.0	25.0	41.0
37	35.58372164868831	39.0	35.0	41.0	25.0	41.0
38	35.47532373915506	39.0	35.0	41.0	24.0	41.0
39	35.36033680457225	39.0	35.0	41.0	24.0	41.0
40	35.277823546070074	39.0	35.0	41.0	23.0	41.0
41	35.133740313570016	39.0	35.0	41.0	23.0	41.0
42	35.04760027546791	39.0	35.0	41.0	23.0	41.0
43	34.94999259840898	38.0	35.0	41.0	22.0	41.0
44	34.76066150915223	38.0	34.0	40.0	22.0	41.0
45	34.69195060886131	38.0	34.0	40.0	22.0	41.0
46	34.58504106273975	38.0	34.0	40.0	21.0	41.0
47	34.480723682516796	38.0	34.0	40.0	20.0	41.0
48	34.35078875215611	38.0	34.0	40.0	20.0	41.0
49	34.27150001287233	38.0	34.0	40.0	20.0	41.0
50	34.186875691887856	38.0	34.0	40.0	20.0	41.0
51	32.94880512576268	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	4.0
12	3.0
13	8.0
14	13.0
15	18.0
16	27.0
17	78.0
18	146.0
19	308.0
20	591.0
21	1016.0
22	1628.0
23	2599.0
24	4431.0
25	7824.0
26	11407.0
27	12590.0
28	11802.0
29	11486.0
30	11981.0
31	13526.0
32	16213.0
33	20758.0
34	32538.0
35	44308.0
36	45974.0
37	64892.0
38	113201.0
39	191973.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.79964214916459	19.73956053857838	23.024579718353372	17.436217593903663
2	37.88584815796926	22.514030842107974	23.862246736863785	15.737874263058982
3	29.252535849445206	22.451600030893598	32.65388873156038	15.641975388100814
4	25.52937466210128	24.366037638699378	32.70312540226038	17.40146229693896
5	23.206562314960223	28.35806966506191	30.613302268104935	17.822065751872923
6	23.518394562726876	33.131934968977674	29.299358957855986	14.050311510439462
7	74.55751358031048	5.562778364184022	15.26916046649332	4.610547589012177
8	75.44747444842056	5.126728110599078	14.893931982596605	4.53186545838375
9	68.8932046958268	7.106653708518909	17.40805936719615	6.5920822284581515
10	35.11025152537137	24.22363746363566	25.11118476945653	15.55492624153644
11	26.608880621991094	23.697480884586668	30.875093324408514	18.818545169013724
12	22.932059830600107	22.37919316221713	34.6571454316093	20.03160157557346
13	21.859955461730557	22.98033107638442	36.64913884097521	18.510574620909818
14	19.61260716216564	25.507813505650955	35.347906958782794	19.531672373400614
15	19.64527070514636	24.193709291249387	37.051560126663745	19.109459876940505
16	22.04419071647401	24.06080246118992	34.61450583116649	19.280500991169582
17	21.415538192209667	24.830889735602295	33.267898977936824	20.485673094251215
18	22.309199855829878	23.66288649177458	34.193741472079914	19.83417218031563
19	21.57692505728188	26.09414823777772	32.31132379064439	20.017602914296013
20	22.35264397703576	25.46597842597122	32.67335813402673	19.5080194629663
21	21.62455268645573	26.15818809051824	33.262910949205775	18.95434827382025
22	20.97836160955642	23.888635017892543	32.62878768375254	22.504215688798496
23	20.186713178693715	25.386974487037563	32.962985608732595	21.463326725536135
24	20.53072625698324	24.515034884020288	33.7053973688953	21.248841490101174
25	20.90740287825348	26.20983832350745	31.832955744921865	21.049803053317202
26	20.07021857220091	26.160279844502227	32.75429292279175	21.01520866050511
27	21.11947455139922	24.844888396879746	33.06338979996396	20.97224725175707
28	19.782843755631646	25.640881239863038	33.15397883788585	21.422296166619468
29	21.61457662899364	24.49202559019643	32.153637721082305	21.739760059727622
30	22.448542851993924	24.312134747573566	32.76587802177999	20.473444378652523
31	22.09455371624231	25.569761604407486	31.530295033854234	20.80538964549597
32	21.840807867569445	26.60212264758129	30.856750251010478	20.700319233838787
33	21.727048631671085	26.295761141003528	30.430354246582397	21.54683598074299
34	21.228406662719152	26.647497618618544	30.98869165615426	21.135404062508044
35	21.70548747522076	25.675797440980357	30.699868702211468	21.918846381587418
36	23.07059830600108	26.638326081919523	29.41295728960173	20.87811832247767
37	21.62230002831913	27.767068712509335	30.361969981721288	20.248661277450246
38	21.431789511623716	27.848325309579593	29.119629019385734	21.600256159410964
39	23.218951934711534	25.679980948948327	29.49260484514584	21.608462271194295
40	23.061909481759905	24.61608269186211	30.685870040934017	21.636137785443967
41	21.472659166387768	24.82364904873465	29.693895940066422	24.009795844811162
42	22.034375563164534	25.729861236258785	29.883923744303992	22.35183945627269
43	22.33317457456942	24.985840434569937	30.529149396287618	22.151835594573026
44	22.458840717761245	25.615297479597356	29.320920114306308	22.604941688335092
45	21.968887573050484	24.6170481167778	30.020531369873595	23.393532940298122
46	22.72191900728574	24.925501377339547	29.48327240429421	22.869307211080503
47	21.547479597353448	24.888171613933014	31.32031511469248	22.24403367402106
48	21.088259145792033	24.4368354658497	31.117575882398373	23.35732950595989
49	22.12625183430734	24.058227994748087	31.21685374456144	22.598666426383133
50	20.883589063666555	23.649048734649742	31.69168189892645	23.775680302757255
51	21.036769816955434	23.158291069175913	30.357303761295473	25.44763535257318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	468.0
2	708.0
3	8099.0
4	15490.0
5	10202.0
6	4914.0
7	4817.0
8	4720.0
9	4720.5
10	4721.0
11	4657.0
12	4593.0
13	4571.0
14	4549.0
15	4284.0
16	4019.0
17	3877.5
18	3736.0
19	3627.5
20	3519.0
21	3424.5
22	3330.0
23	3345.0
24	3360.0
25	3716.5
26	4487.0
27	4901.0
28	5932.5
29	6964.0
30	8162.0
31	9360.0
32	10521.0
33	11682.0
34	13050.5
35	14419.0
36	15982.0
37	17545.0
38	19268.5
39	20992.0
40	23738.0
41	26484.0
42	29410.0
43	32336.0
44	35918.0
45	39500.0
46	47334.0
47	55168.0
48	58878.5
49	62589.0
50	61187.5
51	59786.0
52	52526.0
53	45266.0
54	39420.0
55	33574.0
56	30393.5
57	27213.0
58	24913.0
59	22613.0
60	20561.0
61	18509.0
62	16804.5
63	15100.0
64	13475.0
65	11850.0
66	10191.5
67	8533.0
68	6999.0
69	5465.0
70	4419.0
71	3373.0
72	2959.0
73	2545.0
74	2121.5
75	1419.5
76	1141.0
77	837.0
78	533.0
79	357.5
80	182.0
81	137.0
82	92.0
83	84.5
84	77.0
85	48.0
86	19.0
87	14.5
88	10.0
89	7.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	621488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.82214468533018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88995140673465	32.069483346165384
2	8.65224257284587	7.410151666139586
3	3.569147888193706	4.5851570181471475
4	2.216591234044674	3.7967676212998245
5	1.7992728672950709	3.852436152584921
6	1.416915574526129	3.6405218243553383
7	1.1322119460138254	3.3938620636664294
8	0.9302906548549708	3.18696328172881
9	0.8332768019793508	3.211442979956009
>10	4.495971708484822	28.967254388436842
>50	0.04111694498924251	1.1800026274959126
>100	0.018483763964695678	1.4977391131150612
>500	0.002640537709242236	0.8131599110678616
>1k	0.0015088786909955637	0.7737457238806231
>5k	0.0	0.0
>10k+	3.772196727488909E-4	1.6213122819602952
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10037	1.6149949797904386	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTT	1296	0.20853178178822437	No Hit
CGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTG	1221	0.19646397034214658	No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT	1139	0.18326982982776818	No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	1134	0.18246530906469632	No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	934	0.15028447854182223	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCT	905	0.14561825811600546	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTC	902	0.14513554565816236	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2180830522874135E-4	0.0	0.0	0.20917539839868188	0.0
2	3.2180830522874135E-4	0.0	0.0	0.6975195015832969	0.0
3	3.2180830522874135E-4	0.0	0.0	1.1319607136420977	0.0
4	3.2180830522874135E-4	0.0	0.0	1.5454843858610303	0.0
5	3.2180830522874135E-4	0.0	0.0	2.70882140926293	0.0
6	3.2180830522874135E-4	0.0	0.0	3.9501969466828	0.0
7	3.2180830522874135E-4	0.0	0.0	4.986419689519347	0.0
8	3.2180830522874135E-4	0.0	0.0	6.472530443065675	0.0
9	3.2180830522874135E-4	0.0	0.0	7.251789254177072	0.0
10	3.2180830522874135E-4	0.0	0.0	8.506680740416549	0.0
11	3.2180830522874135E-4	0.0	0.0	9.898984372988698	0.0
12	3.2180830522874135E-4	0.0	0.0	11.075354632752362	0.0
13	3.2180830522874135E-4	0.0	0.0	11.655092294621939	0.0
14	3.2180830522874135E-4	0.0	0.0	11.942306207038591	0.0
15	4.82712457843112E-4	0.0	0.0	12.294042684653606	0.0
16	4.82712457843112E-4	0.0	0.0	12.94763535257318	0.0
17	4.82712457843112E-4	0.0	0.0	13.704850294776408	0.0
18	4.82712457843112E-4	0.0	0.0	14.501969466828	0.0
19	4.82712457843112E-4	0.0	0.0	15.028930566640064	0.0
20	4.82712457843112E-4	0.0	0.0	15.528859768812914	0.0
21	4.82712457843112E-4	0.0	0.0	16.12291790026517	0.0
22	4.82712457843112E-4	0.0	0.0	16.76492546919651	0.0
23	4.82712457843112E-4	0.0	0.0	17.418196328810854	0.0
24	4.82712457843112E-4	0.0	0.0	17.95802976083207	0.0
25	4.82712457843112E-4	0.0	0.0	18.45586720902093	0.0
26	4.82712457843112E-4	0.0	0.0	18.924259197281362	0.0
27	4.82712457843112E-4	0.0	0.0	19.398121926730685	0.0
28	4.82712457843112E-4	0.0	0.0	19.866031202533275	0.0
29	4.82712457843112E-4	0.0	0.0	20.383981669798935	0.0
30	4.82712457843112E-4	0.0	0.0	20.982706021677007	0.0
31	6.436166104574827E-4	0.0	0.0	21.494381226990708	0.0
32	6.436166104574827E-4	0.0	0.0	22.023434080786757	0.0
33	6.436166104574827E-4	0.0	0.0	22.497618618541306	0.0
34	6.436166104574827E-4	0.0	0.0	22.95716087840795	0.0
35	6.436166104574827E-4	0.0	0.0	23.446148598203024	0.0
36	6.436166104574827E-4	0.0	0.0	23.915666915531755	0.0
37	8.045207630718534E-4	0.0	0.0	24.407550910073887	0.0
38	8.045207630718534E-4	0.0	0.0	24.894929588342816	0.0
39	8.045207630718534E-4	0.0	0.0	25.3761939088124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	30	2.1638862E-6	45.000004	1
GCCGATA	20	7.030783E-4	45.0	9
CCGACGG	20	7.030783E-4	45.0	2
TATAGCG	25	3.888731E-5	45.0	1
GCTACGA	50	2.1827873E-11	45.0	10
CCGCATG	20	7.030783E-4	45.0	1
CGTTTTT	5385	0.0	43.87187	1
AAACACG	135	0.0	41.666668	40
AATACGG	55	6.002665E-11	40.90909	2
ACACGAC	150	0.0	40.500004	26
CGAATAT	50	1.0804797E-9	40.5	14
ATATCGG	40	3.4548066E-7	39.375	2
ACGATAG	40	3.4548066E-7	39.375	1
TCTACGG	40	3.4548066E-7	39.375	2
GCGAGAC	155	0.0	39.193546	21
GACACGA	155	0.0	39.193546	25
CACGACC	155	0.0	39.193546	27
ACGACCA	155	0.0	39.193546	28
ATCGAGG	35	6.2436593E-6	38.571426	2
AACACGT	140	0.0	38.571426	41
>>END_MODULE