FastQCFastQC Report
Sat 18 Jun 2016
SRR3550470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193020
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99250.8319223483260968No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC20780.17417981257648657TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC19900.16680357412281438TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT19010.15934351477762318TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT17410.14593217213458284No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTG14850.12447402390571825TruSeq Adapter, Index 21 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC12690.10636871133761379No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCT12360.1036026219174867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTT253.8908955E-545.023
AATTGCG253.8908955E-545.01
TATCGCG207.033394E-445.01
TGCGTCG207.033394E-445.01
CGTTTTT55050.042.1389661
CGTATGG1250.041.3999982
GCGATAC501.0822987E-940.59
GTATACG403.4583718E-739.3751
CGTAAGG1900.037.8947372
TACGGGT900.037.54
TCGACGG301.1399481E-437.4999962
AATACGG601.5643309E-1037.4999962
GCTACGA1150.037.17391210
CGAATAT1100.036.8181814
GCGTAAG1100.036.818181
CGCATGG1600.036.56252
GCGTATG1050.036.428571
AACACGT5450.036.33027641
CAACCCG250.002107140636.023
TCTAGCG504.879257E-836.01
Produced by FastQC (version 0.11.5)