##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1193020
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24601766944393	33.0	31.0	34.0	30.0	34.0
2	32.36576168044123	34.0	31.0	34.0	30.0	34.0
3	32.42816046671472	34.0	31.0	34.0	30.0	34.0
4	35.9373631623946	37.0	35.0	37.0	35.0	37.0
5	35.92017736500645	37.0	35.0	37.0	35.0	37.0
6	35.9497074650886	37.0	35.0	37.0	35.0	37.0
7	36.153603460126405	37.0	35.0	37.0	35.0	37.0
8	36.15931920671908	37.0	36.0	37.0	35.0	37.0
9	37.74129687683358	39.0	38.0	39.0	35.0	39.0
10	37.453949640408375	39.0	37.0	39.0	35.0	39.0
11	37.42247070459842	39.0	37.0	39.0	35.0	39.0
12	37.42382022095187	39.0	37.0	39.0	35.0	39.0
13	37.419192469531104	39.0	37.0	39.0	35.0	39.0
14	38.66141221438031	40.0	38.0	41.0	35.0	41.0
15	38.712984694305206	40.0	38.0	41.0	35.0	41.0
16	38.72412784362375	40.0	38.0	41.0	35.0	41.0
17	38.73338837571876	40.0	38.0	41.0	35.0	41.0
18	38.63801277430387	40.0	38.0	41.0	35.0	41.0
19	38.54635546763675	40.0	37.0	41.0	35.0	41.0
20	38.41100065380295	40.0	37.0	41.0	34.0	41.0
21	38.36269886506513	40.0	37.0	41.0	34.0	41.0
22	38.353318469095235	40.0	37.0	41.0	34.0	41.0
23	38.31442725184825	40.0	37.0	41.0	34.0	41.0
24	38.29109822132068	40.0	37.0	41.0	34.0	41.0
25	38.25692109101272	40.0	37.0	41.0	34.0	41.0
26	38.14826406933664	40.0	37.0	41.0	34.0	41.0
27	38.10461014903355	40.0	37.0	41.0	34.0	41.0
28	38.026296290087345	40.0	37.0	41.0	34.0	41.0
29	37.98387872793415	40.0	37.0	41.0	34.0	41.0
30	37.9431300397311	40.0	37.0	41.0	33.0	41.0
31	37.81898459372014	40.0	37.0	41.0	33.0	41.0
32	37.60985733684264	40.0	36.0	41.0	33.0	41.0
33	37.4474065816164	40.0	36.0	41.0	33.0	41.0
34	37.23103803792057	40.0	36.0	41.0	32.0	41.0
35	37.15297815627567	40.0	36.0	41.0	31.0	41.0
36	36.99916011466698	40.0	36.0	41.0	31.0	41.0
37	36.97998440931418	40.0	36.0	41.0	31.0	41.0
38	36.88069521047426	40.0	35.0	41.0	31.0	41.0
39	36.800932088313694	40.0	35.0	41.0	31.0	41.0
40	36.703326012975474	39.0	35.0	41.0	30.0	41.0
41	36.5860169988768	39.0	35.0	41.0	30.0	41.0
42	36.54178219979548	39.0	35.0	41.0	30.0	41.0
43	36.488199694891954	39.0	35.0	41.0	30.0	41.0
44	36.32083200616922	39.0	35.0	41.0	30.0	41.0
45	36.26791923018893	39.0	35.0	41.0	30.0	41.0
46	36.151551524702015	39.0	35.0	41.0	29.0	41.0
47	36.05476018842936	39.0	35.0	41.0	29.0	41.0
48	35.95354897654691	39.0	35.0	41.0	29.0	41.0
49	35.87673383514107	39.0	35.0	41.0	28.0	41.0
50	35.79750129922382	39.0	35.0	40.0	28.0	41.0
51	34.594520628321405	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	7.0
12	12.0
13	12.0
14	23.0
15	24.0
16	51.0
17	121.0
18	176.0
19	381.0
20	733.0
21	1239.0
22	1966.0
23	3024.0
24	4958.0
25	8058.0
26	11865.0
27	14288.0
28	15024.0
29	15897.0
30	18574.0
31	21934.0
32	26914.0
33	35763.0
34	56726.0
35	77361.0
36	84315.0
37	122369.0
38	225989.0
39	444887.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07652847395685	21.145747766173244	24.778629025498315	13.999094734371594
2	33.868334143601956	23.47026872977821	24.941576838611258	17.719820288008584
3	30.743994233122663	23.7750414913413	29.086436103334396	16.39452817220164
4	27.29149553234648	25.640726894771255	28.45736031248428	18.61041726039798
5	24.844260783557694	29.68097768687868	26.526629897235587	18.948131632328042
6	26.00224639989271	33.32978491559236	25.463110425642487	15.204858258872441
7	80.56377931635681	5.105530502422424	9.975943404134046	4.354746777086721
8	81.76971048264069	4.160114666979598	9.735293624582992	4.3348812257967175
9	74.53613518633384	7.333908903455097	12.239778042279257	5.890177867931803
10	33.14898325258587	35.76117751588406	17.966840455315083	13.122998776214983
11	24.27419490033696	24.74551977334831	32.7454694808134	18.23481584550133
12	23.6559320044928	22.688219811905917	34.09766810279794	19.55818008080334
13	22.284706040133443	23.340430168815278	35.163869842919645	19.210993948131634
14	19.40830832676736	26.793096511374493	32.73616536185479	21.06242980000335
15	18.564231949171013	26.2990561767615	35.71163936899633	19.425072505071164
16	21.588657356959647	25.94172771621599	33.66548758612596	18.804127340698397
17	21.00911971299727	25.31416070141322	31.80516671975323	21.87155286583628
18	21.876246835761346	24.906372064173272	32.07272300548188	21.144658094583495
19	21.834420210893363	26.84716098640425	30.559001525540225	20.75941727716216
20	24.568238587785622	25.47048666409616	30.496303498684014	19.46497124943421
21	23.122998776214985	26.65982129385928	30.59395483730365	19.623225092622086
22	21.731991081457142	24.728923236827548	30.06035104189368	23.47873463982163
23	20.4838980067392	26.957553100534774	30.983302878409415	21.57524601431661
24	20.62765083569429	25.38658195168564	32.21094365559672	21.77482355702335
25	21.00878442943119	27.003570769978708	29.902516303163402	22.085128497426698
26	20.733097517225193	27.167608254681397	30.273339927243466	21.825954300849943
27	21.70189938140182	25.80828485691774	30.826138706811285	21.663677054869154
28	19.525741395785488	26.790665705520446	31.538113359373693	22.14547953932038
29	20.74516772560393	24.61283130207373	31.229820120366803	23.41218085195554
30	22.17062580677608	23.944778796667283	31.76560325895626	22.118992137600376
31	21.495364704699	25.525892273390223	30.487753767749076	22.490989254161708
32	20.95371410370321	25.74407805401418	31.052036009454998	22.250171832827615
33	21.415064290623796	25.650701580862012	30.589177046487066	22.345057082027125
34	21.384050560761764	25.40166971215906	31.23174800087174	21.98253172620744
35	21.199393136745403	25.13042530720357	31.142478751404003	22.527702804647028
36	20.943152671371813	27.08990628824328	29.552899364637643	22.414041675747264
37	21.56074500008382	26.30266047509681	30.863522824428763	21.273071700390606
38	21.186652361234515	26.66912541281789	30.088682503227105	22.05553972272049
39	23.211848921225126	24.284504869993796	28.906556470134614	23.59708973864646
40	23.58954585840975	24.783406816314898	30.297647985783975	21.329399339491374
41	19.98600191111633	26.53761043402458	29.75230926556135	23.72407838929775
42	21.567953596754457	26.105681380027157	29.51777841109118	22.808586612127208
43	21.82570283817539	24.77661732410186	31.016747414125494	22.380932423597258
44	21.92201304253072	25.178370857152437	30.18901611037535	22.710599989941493
45	22.10532933228278	23.637910512816216	29.942079763960372	24.314680390940637
46	22.37640609545523	24.71542807329299	29.196325292115805	23.711840539135977
47	20.781881276089255	25.611808687197197	30.72924175621532	22.877068280498232
48	20.894285091616236	24.407721580526733	30.752292501383042	23.94570082647399
49	21.350103099696568	23.93379825987829	30.6365358501953	24.079562790229836
50	19.925734690114165	23.747045313573956	31.736265946924615	24.59095404938727
51	20.258419808553082	23.112018239425993	29.84979296239795	26.779768989622976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	281.0
1	690.5
2	1100.0
3	8588.5
4	16077.0
5	10556.5
6	5036.0
7	4988.5
8	4941.0
9	4820.0
10	4699.0
11	4544.5
12	4390.0
13	4309.0
14	4228.0
15	4199.0
16	4170.0
17	3950.5
18	3731.0
19	3840.5
20	3950.0
21	4299.5
22	4649.0
23	5184.5
24	5720.0
25	6731.5
26	9881.5
27	12020.0
28	14423.5
29	16827.0
30	19338.5
31	21850.0
32	24458.5
33	27067.0
34	31152.5
35	35238.0
36	39678.5
37	44119.0
38	47424.5
39	50730.0
40	54782.0
41	58834.0
42	63807.5
43	68781.0
44	75547.0
45	82313.0
46	93513.0
47	104713.0
48	111416.5
49	118120.0
50	115334.5
51	112549.0
52	98844.5
53	85140.0
54	75309.0
55	65478.0
56	58181.0
57	50884.0
58	46602.5
59	42321.0
60	41015.5
61	39710.0
62	35216.5
63	30723.0
64	25507.5
65	20292.0
66	16177.5
67	12063.0
68	9960.5
69	7858.0
70	6451.5
71	5045.0
72	4273.5
73	3502.0
74	3040.0
75	2162.5
76	1747.0
77	1421.5
78	1096.0
79	776.5
80	457.0
81	293.0
82	129.0
83	92.5
84	56.0
85	39.0
86	22.0
87	18.0
88	14.0
89	15.0
90	16.0
91	12.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1193020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.67935962398415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49227737123965	23.363218425523648
2	9.839830029665912	6.431186883566618
3	3.6243482650346333	3.553241410668894
4	2.0255171226452786	2.647704099018507
5	1.3994956235520053	2.286731038712396
6	1.085105951121711	2.1276340564099048
7	0.8994385032409745	2.0575152014948888
8	0.8317286372159298	2.174428739611649
9	0.7719136138637163	2.270307832749146
>10	7.91973669862625	47.27373851273143
>50	0.07330926312530327	1.6150820219445912
>100	0.03367263677636954	1.9617596147906338
>500	0.0015541216681090205	0.4069787764777073
>1k	0.0018131419461271906	0.9903601673854359
>5k	2.590202780181701E-4	0.8401132189145686
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9925	0.8319223483260968	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	2078	0.17417981257648657	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1990	0.16680357412281438	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	1901	0.15934351477762318	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT	1741	0.14593217213458284	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTG	1485	0.12447402390571825	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC	1269	0.10636871133761379	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCT	1236	0.1036026219174867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.19104457595011E-4	0.0	0.0	0.17845467804395568	0.0
2	4.19104457595011E-4	0.0	0.0	0.6826373405307539	0.0
3	4.19104457595011E-4	0.0	0.0	1.0548020988751237	0.0
4	4.19104457595011E-4	0.0	0.0	1.453370438047979	0.0
5	4.19104457595011E-4	0.0	0.0	2.603644532363246	0.0
6	4.19104457595011E-4	0.0	0.0	3.701195285913061	0.0
7	4.19104457595011E-4	0.0	0.0	4.665889926405257	0.0
8	4.19104457595011E-4	0.0	0.0	6.175588003554005	0.0
9	4.19104457595011E-4	0.0	0.0	6.947326951769459	0.0
10	4.19104457595011E-4	0.0	0.0	8.163148983252587	0.0
11	4.19104457595011E-4	0.0	0.0	9.509144859264723	0.0
12	4.19104457595011E-4	0.0	0.0	10.537375735528324	0.0
13	4.19104457595011E-4	0.0	0.0	11.014400429162965	0.0
14	5.029253491140132E-4	0.0	0.0	11.245662268863892	0.0
15	5.029253491140132E-4	0.0	0.0	11.569797656367873	0.0
16	5.029253491140132E-4	0.0	0.0	12.182779836046336	0.0
17	5.029253491140132E-4	0.0	0.0	12.974971081792425	0.0
18	5.029253491140132E-4	0.0	0.0	13.797337848485357	0.0
19	5.867462406330153E-4	0.0	0.0	14.31442892826608	0.0
20	7.543880236710197E-4	0.0	0.0	14.800422457293255	0.0
21	7.543880236710197E-4	0.0	0.0	15.395969891535767	0.0
22	7.543880236710197E-4	0.0	0.0	16.075757321754875	0.0
23	8.38208915190022E-4	0.0	0.0	16.744564215184994	0.0
24	8.38208915190022E-4	0.0	0.0	17.278335652377997	0.0
25	8.38208915190022E-4	0.0	0.0	17.72845383983504	0.0
26	8.38208915190022E-4	0.0	0.0	18.173291311126384	0.0
27	8.38208915190022E-4	0.0	0.0	18.61896699133292	0.0
28	8.38208915190022E-4	0.0	0.0	19.089202192754524	0.0
29	8.38208915190022E-4	0.0	0.0	19.593971601481954	0.0
30	8.38208915190022E-4	0.0	0.0	20.18909993126687	0.0
31	9.220298067090241E-4	0.0	0.0	20.69470754890949	0.0
32	9.220298067090241E-4	0.0	0.0	21.212804479388442	0.0
33	9.220298067090241E-4	0.0	0.0	21.684464635965867	0.0
34	0.0010058506982280264	0.0	0.0	22.15704682235	0.0
35	0.0010058506982280264	0.0	0.0	22.655026738864393	0.0
36	0.0010896715897470285	0.0	0.0	23.168178236743724	0.0
37	0.0010896715897470285	0.0	0.0	23.670684481400144	0.0
38	0.0010896715897470285	0.0	0.0	24.160282308762635	0.0
39	0.0010896715897470285	0.0	0.0	24.654490285158673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTT	25	3.8908955E-5	45.0	23
AATTGCG	25	3.8908955E-5	45.0	1
TATCGCG	20	7.033394E-4	45.0	1
TGCGTCG	20	7.033394E-4	45.0	1
CGTTTTT	5505	0.0	42.138966	1
CGTATGG	125	0.0	41.399998	2
GCGATAC	50	1.0822987E-9	40.5	9
GTATACG	40	3.4583718E-7	39.375	1
CGTAAGG	190	0.0	37.894737	2
TACGGGT	90	0.0	37.5	4
TCGACGG	30	1.1399481E-4	37.499996	2
AATACGG	60	1.5643309E-10	37.499996	2
GCTACGA	115	0.0	37.173912	10
CGAATAT	110	0.0	36.81818	14
GCGTAAG	110	0.0	36.81818	1
CGCATGG	160	0.0	36.5625	2
GCGTATG	105	0.0	36.42857	1
AACACGT	545	0.0	36.330276	41
CAACCCG	25	0.0021071406	36.0	23
TCTAGCG	50	4.879257E-8	36.0	1
>>END_MODULE