FastQCFastQC Report
Sat 18 Jun 2016
SRR3550469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567831
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC24380.4293530997779269No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC13950.2456716875267465No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC12920.22753248765918027No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG11510.20270115580163817No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC11090.19530458886534902No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10430.18368141225118037No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT10140.1785742588904093No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.16536610364703583No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC7160.12609385539007206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG207.030269E-445.01
GGTCGAT207.030269E-445.08
ACGCAAC207.030269E-445.011
ATTACGT207.030269E-445.017
TAGCACG207.030269E-445.01
AATGCGA253.888304E-545.025
TTACGTC207.030269E-445.018
ATGCGAA253.888304E-545.026
CGCACGA207.030269E-445.039
CGTCACT207.030269E-445.033
CTAAGCG302.163555E-644.9999961
GCGTAAG302.163555E-644.9999961
TACGAGG603.6379788E-1241.2499962
TGCGAAG603.6379788E-1241.2499961
TACACGG501.0786607E-940.52
ATAGCGG451.9254003E-840.02
TATCCGG451.9254003E-840.010
TCACGGG850.039.7058833
CGTAAGG850.039.7058832
GGTACGC403.4540972E-739.3758