##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567831
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.157937837138164	33.0	31.0	34.0	30.0	34.0
2	32.25929722047581	34.0	31.0	34.0	30.0	34.0
3	32.376779358647205	34.0	31.0	34.0	30.0	34.0
4	35.88092231667521	37.0	35.0	37.0	35.0	37.0
5	35.85318871283886	37.0	35.0	37.0	35.0	37.0
6	35.86760321292779	37.0	35.0	37.0	35.0	37.0
7	36.05550947376948	37.0	35.0	37.0	35.0	37.0
8	36.00191606305397	37.0	35.0	37.0	35.0	37.0
9	37.58466691674107	39.0	38.0	39.0	35.0	39.0
10	37.355759019849216	39.0	37.0	39.0	34.0	39.0
11	37.3121456912356	39.0	37.0	39.0	34.0	39.0
12	37.19421447578593	39.0	37.0	39.0	34.0	39.0
13	37.08923958008633	39.0	37.0	39.0	33.0	39.0
14	38.23362937211952	40.0	37.0	41.0	33.0	41.0
15	38.305686022777905	40.0	37.0	41.0	34.0	41.0
16	38.35922660087244	40.0	37.0	41.0	34.0	41.0
17	38.36522134226557	40.0	37.0	41.0	34.0	41.0
18	38.33687664111329	40.0	37.0	41.0	34.0	41.0
19	38.30886654656051	40.0	37.0	41.0	34.0	41.0
20	38.202229184387605	40.0	37.0	41.0	34.0	41.0
21	38.16584511941053	40.0	37.0	41.0	34.0	41.0
22	38.15284653356368	40.0	37.0	41.0	34.0	41.0
23	38.10840549388815	40.0	37.0	41.0	34.0	41.0
24	38.080393638248	40.0	37.0	41.0	34.0	41.0
25	38.03748298349333	40.0	37.0	41.0	34.0	41.0
26	37.90869642552097	40.0	36.0	41.0	34.0	41.0
27	37.86131260885721	40.0	36.0	41.0	33.0	41.0
28	37.79546203007585	40.0	36.0	41.0	33.0	41.0
29	37.73150638130007	40.0	36.0	41.0	33.0	41.0
30	37.71270501258297	40.0	36.0	41.0	33.0	41.0
31	37.60459538137228	40.0	36.0	41.0	33.0	41.0
32	37.44942245139839	40.0	36.0	41.0	33.0	41.0
33	37.34759461882145	40.0	35.0	41.0	32.0	41.0
34	37.179047991391805	39.0	35.0	41.0	32.0	41.0
35	37.17082899665569	39.0	35.0	41.0	32.0	41.0
36	37.04695058917178	39.0	35.0	41.0	31.0	41.0
37	37.01370654296789	39.0	35.0	41.0	31.0	41.0
38	36.93694426686813	39.0	35.0	41.0	31.0	41.0
39	36.81646651908755	39.0	35.0	41.0	31.0	41.0
40	36.707629558794785	39.0	35.0	41.0	31.0	41.0
41	36.59511544808226	39.0	35.0	41.0	31.0	41.0
42	36.597959604178	39.0	35.0	41.0	31.0	41.0
43	36.518279206313146	39.0	35.0	41.0	31.0	41.0
44	36.33404833480384	39.0	35.0	41.0	30.0	41.0
45	36.26213081004735	38.0	35.0	40.0	30.0	41.0
46	36.11929429707078	38.0	35.0	40.0	30.0	41.0
47	36.03900104080263	38.0	35.0	40.0	30.0	41.0
48	35.955750918847336	38.0	35.0	40.0	30.0	41.0
49	35.872093985710535	38.0	35.0	40.0	29.0	41.0
50	35.777188987568486	38.0	35.0	40.0	29.0	41.0
51	34.42368063737274	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	4.0
10	5.0
11	1.0
12	7.0
13	9.0
14	11.0
15	18.0
16	26.0
17	57.0
18	119.0
19	216.0
20	354.0
21	622.0
22	1011.0
23	1446.0
24	2110.0
25	3072.0
26	4080.0
27	5025.0
28	6065.0
29	7134.0
30	9063.0
31	11329.0
32	14630.0
33	20165.0
34	34754.0
35	46401.0
36	43490.0
37	63432.0
38	108585.0
39	184449.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.552868723264496	20.603137201033405	23.966637960942606	10.877356114759497
2	32.35681038900659	24.99933959223783	23.77309445944304	18.870755559312542
3	32.230188207406776	24.918505682148385	25.401043620372967	17.450262490071868
4	28.97622708164929	26.059161968966116	25.47958811688689	19.485022832497698
5	25.760833769202456	29.444852429684182	23.523724488448146	21.270589312665212
6	26.27383851885508	34.17354811554846	23.920497471959088	15.632115893637366
7	79.33557695863735	6.088079023512277	9.213656880304175	5.362687137546207
8	78.18593912625411	6.59509607612124	8.862143842093863	6.356820955530782
9	71.61937266545856	8.00326153380143	11.922737575088362	8.454628225651646
10	39.352201623370334	25.66960944365489	18.721063133220976	16.257125799753798
11	30.607698417310786	24.256512941350508	24.7413402931506	20.394448348188103
12	28.34822332701103	22.05814758264343	28.66398629169594	20.9296427986496
13	24.728660464116963	24.128834107331233	30.33666707171676	20.805838356835043
14	20.24493203083312	28.102199422011125	28.871266274648622	22.781602272507136
15	19.248508799273022	24.96323730123928	34.66630036049458	21.121953538993115
16	21.72829591903225	24.821469768293735	31.190970552858154	22.259263759815862
17	21.459025660804006	24.420646283841496	28.582271837923606	25.53805621743089
18	23.069187839339524	24.321673173884484	29.916471626240902	22.692667360535086
19	23.773270568179615	26.60668403098809	26.675718655726794	22.9443267451055
20	26.93759234701874	25.712932192853156	26.55068145275619	20.798794007371914
21	23.990412640380676	26.95995815656419	27.241027700143178	21.80860150291196
22	22.34080210485162	25.169108414299323	25.86544235872997	26.624647122119082
23	22.19375130980873	27.21232197608091	27.419425850297007	23.174500863813353
24	23.430915184271374	23.830505907567566	28.451070829172764	24.28750807898829
25	21.650984183674368	26.345514774642453	26.47548302223725	25.528018019445923
26	21.045522347318126	26.485345111485636	28.030347057487177	24.43878548370906
27	23.13100200587851	25.671194422284096	26.89303683666443	24.304766735172965
28	19.74672041505307	26.30958859238048	27.866565932469346	26.077125060097107
29	21.32218917248266	24.746271337774793	28.881304472633584	25.050235017108964
30	23.426160248383763	23.835789169664917	27.474547884846018	25.2635026971053
31	22.366337871655475	26.189130216560912	25.462857786911954	25.981674124871663
32	22.967749207070415	25.199927443200533	27.324327132544717	24.50799621718434
33	23.0880314741534	25.160479086206987	25.840434918135852	25.911054521503758
34	21.494951843065984	25.42745993085971	28.582624055396767	24.494964170677545
35	20.65685036568979	23.834204191035713	28.683886578929297	26.825058864345202
36	21.872705083026464	26.293034371142117	26.976512377802553	24.857748168028866
37	20.958172413975284	26.819423384774694	27.609975503274743	24.61242869797528
38	21.591987756920634	26.05458314181508	26.12150446171484	26.23192463954944
39	23.15072618437528	23.97544339777152	29.179632672397243	23.694197745455956
40	24.165112507066365	23.073062231544245	29.273674737730065	23.48815052365933
41	19.064651278285265	25.45951172091696	27.783970935014114	27.69186606578366
42	21.3803050555535	23.465784714113884	29.29251837254394	25.861391857788675
43	22.380074353108583	22.936225743222895	29.30431765789469	25.37938224577383
44	22.130352164640534	23.77784939533065	27.91517194376496	26.176626496263854
45	22.623104409586656	22.65691728700969	27.500964195332767	27.219014108070887
46	22.34273930095398	24.208611365001207	27.529317701921872	25.919331632122937
47	20.09858567073654	24.05240291565624	30.32733330867811	25.52167810492911
48	20.5642171702496	22.69953560126164	29.373704500106545	27.36254272838221
49	21.612944696573454	21.84065329296921	31.101155097203215	25.44524691325412
50	20.225031743599768	22.52342686468333	30.017734149773435	27.233807241943463
51	20.013349042232637	22.11626346571427	27.408154891155995	30.4622326008971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	464.5
2	740.0
3	1296.5
4	1853.0
5	1311.5
6	770.0
7	807.5
8	845.0
9	937.5
10	1030.0
11	1000.5
12	971.0
13	1014.5
14	1058.0
15	1083.0
16	1108.0
17	1054.5
18	1001.0
19	1054.5
20	1108.0
21	1254.0
22	1400.0
23	1576.0
24	1752.0
25	2081.0
26	2757.0
27	3104.0
28	4185.0
29	5266.0
30	6103.0
31	6940.0
32	8121.5
33	9303.0
34	10839.0
35	12375.0
36	13369.0
37	14363.0
38	16109.0
39	17855.0
40	20090.5
41	22326.0
42	24927.0
43	27528.0
44	31170.0
45	34812.0
46	43245.5
47	51679.0
48	55812.0
49	59945.0
50	60186.0
51	60427.0
52	53384.5
53	46342.0
54	40995.0
55	35648.0
56	32652.5
57	29657.0
58	28146.5
59	26636.0
60	25301.5
61	23967.0
62	22589.0
63	21211.0
64	17725.5
65	14240.0
66	11927.5
67	9615.0
68	8175.5
69	6736.0
70	5505.5
71	4275.0
72	3676.5
73	3078.0
74	2504.5
75	1597.5
76	1264.0
77	847.0
78	430.0
79	403.0
80	376.0
81	269.0
82	162.0
83	101.5
84	41.0
85	34.5
86	28.0
87	23.0
88	18.0
89	13.5
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.26493361602637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7712191276936	23.874648398240097
2	10.752474184639054	7.153606799201108
3	3.9642145756498763	3.9560800409603183
4	2.0327905590265076	2.7048257200520767
5	1.226504953104302	2.0399802922371073
6	0.9376519364618363	1.8714557652804935
7	0.7392743173959956	1.7214337764547702
8	0.6613655708375443	1.760022544786904
9	0.5508627563701909	1.649197171997614
>10	7.221032353430791	46.575423726338684
>50	0.09099348052888803	2.0683762610111174
>100	0.046294928690130445	2.567724950126189
>500	0.0015963768513838083	0.3858844885754234
>1k	0.003724879319895553	1.6713400647381411
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2438	0.4293530997779269	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1395	0.2456716875267465	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	1292	0.22753248765918027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	1151	0.20270115580163817	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	1109	0.19530458886534902	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1043	0.18368141225118037	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	1014	0.1785742588904093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	939	0.16536610364703583	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	716	0.12609385539007206	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2019967208553249	0.0
2	0.0	0.0	0.0	1.1825701661233712	0.0
3	0.0	0.0	0.0	1.63886790259778	0.0
4	0.0	0.0	0.0	2.230945474974068	0.0
5	0.0	0.0	0.0	3.981114099089342	0.0
6	0.0	0.0	0.0	4.984933897585726	0.0
7	0.0	0.0	0.0	6.052505058723458	0.0
8	0.0	0.0	0.0	7.8456442145638405	0.0
9	0.0	0.0	0.0	8.563287316120466	0.0
10	0.0	0.0	0.0	10.129422310511401	0.0
11	0.0	0.0	0.0	11.878358173470629	0.0
12	0.0	0.0	0.0	13.451889734797854	0.0
13	0.0	0.0	0.0	14.03886015381337	0.0
14	0.0	0.0	0.0	14.301086062578479	0.0
15	0.0	0.0	0.0	14.788555045427248	0.0
16	0.0	0.0	0.0	15.706785997946572	0.0
17	0.0	0.0	0.0	16.7248706041058	0.0
18	0.0	0.0	0.0	17.86059584629934	0.0
19	0.0	0.0	0.0	18.497052820293362	0.0
20	0.0	0.0	0.0	19.116955573049022	0.0
21	0.0	0.0	0.0	19.890249035364395	0.0
22	0.0	0.0	0.0	20.727646077794272	0.0
23	0.0	0.0	0.0	21.535280743742415	0.0
24	0.0	0.0	0.0	22.18529809045297	0.0
25	0.0	0.0	0.0	22.745147764035426	0.0
26	0.0	0.0	0.0	23.278581127131137	0.0
27	0.0	0.0	0.0	23.805850684446604	0.0
28	0.0	0.0	0.0	24.358127682356194	0.0
29	0.0	0.0	0.0	24.946683080000916	0.0
30	0.0	0.0	0.0	25.618185692574023	0.0
31	0.0	0.0	0.0	26.21149602610636	0.0
32	0.0	0.0	0.0	26.784553854932188	0.0
33	1.7610873657831293E-4	0.0	0.0	27.32644043738366	0.0
34	1.7610873657831293E-4	0.0	0.0	27.847722297655466	0.0
35	1.7610873657831293E-4	0.0	0.0	28.422717322583654	0.0
36	1.7610873657831293E-4	0.0	0.0	28.98168645248322	0.0
37	1.7610873657831293E-4	0.0	0.0	29.54752382310934	0.0
38	1.7610873657831293E-4	0.0	0.0	30.099096386072617	0.0
39	1.7610873657831293E-4	0.0	0.0	30.660178820811122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	20	7.030269E-4	45.0	1
GGTCGAT	20	7.030269E-4	45.0	8
ACGCAAC	20	7.030269E-4	45.0	11
ATTACGT	20	7.030269E-4	45.0	17
TAGCACG	20	7.030269E-4	45.0	1
AATGCGA	25	3.888304E-5	45.0	25
TTACGTC	20	7.030269E-4	45.0	18
ATGCGAA	25	3.888304E-5	45.0	26
CGCACGA	20	7.030269E-4	45.0	39
CGTCACT	20	7.030269E-4	45.0	33
CTAAGCG	30	2.163555E-6	44.999996	1
GCGTAAG	30	2.163555E-6	44.999996	1
TACGAGG	60	3.6379788E-12	41.249996	2
TGCGAAG	60	3.6379788E-12	41.249996	1
TACACGG	50	1.0786607E-9	40.5	2
ATAGCGG	45	1.9254003E-8	40.0	2
TATCCGG	45	1.9254003E-8	40.0	10
TCACGGG	85	0.0	39.705883	3
CGTAAGG	85	0.0	39.705883	2
GGTACGC	40	3.4540972E-7	39.375	8
>>END_MODULE