FastQCFastQC Report
Sat 18 Jun 2016
SRR3550468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460602
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC30760.6678216768489933TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC27260.5918341648538218No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG24140.5240967255895546No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC23030.4999978289282287No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC19250.41793131597344346TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT13390.2907065101758134TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC10290.2234032852658043TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.14177098666527718No Hit
ACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC6480.14068545077963188TruSeq Adapter, Index 16 (95% over 23bp)
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC6330.13742884312269596No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT5140.11159308904433764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTA207.0288783E-445.00000415
TGTTTCG207.0288783E-445.0000041
TTGGCGT207.0288783E-445.00000422
TCGCAGG207.0288783E-445.0000042
TCGCAAG207.0288783E-445.0000041
GATCGGC207.0288783E-445.00000421
ACACCGC302.1626583E-645.00000433
AATGCGA302.1626583E-645.00000425
CGGATCG207.0288783E-445.00000419
CTACGAA207.0288783E-445.00000411
CACTACG207.0288783E-445.0000041
ATGCGAA302.1626583E-645.00000426
GCTACGA207.0288783E-445.00000410
ATCGGGT302.1626583E-645.0000044
CGGGTAT502.1827873E-1145.06
CGAACGG253.8871534E-545.02
TCCGACA253.8871534E-545.029
ATTCGGG850.042.352943
ACGGGTA700.041.7857135
CGCAAGG1250.041.3999982
Produced by FastQC (version 0.11.5)