##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	652460
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19582196609754	33.0	31.0	34.0	30.0	34.0
2	32.29946510130889	34.0	31.0	34.0	30.0	34.0
3	32.391446218925296	34.0	31.0	34.0	30.0	34.0
4	35.911861263525736	37.0	35.0	37.0	35.0	37.0
5	35.88312846764553	37.0	35.0	37.0	35.0	37.0
6	35.90557888606198	37.0	35.0	37.0	35.0	37.0
7	36.08734328541213	37.0	35.0	37.0	35.0	37.0
8	36.06925941820188	37.0	35.0	37.0	35.0	37.0
9	37.630742114459125	39.0	38.0	39.0	35.0	39.0
10	37.403807129939	39.0	37.0	39.0	34.0	39.0
11	37.357361370812	39.0	37.0	39.0	34.0	39.0
12	37.290275265916684	39.0	37.0	39.0	34.0	39.0
13	37.22123042025565	39.0	37.0	39.0	34.0	39.0
14	38.39260950862888	40.0	38.0	41.0	34.0	41.0
15	38.45046133096282	40.0	38.0	41.0	34.0	41.0
16	38.47531036385372	40.0	38.0	41.0	34.0	41.0
17	38.4991110566165	40.0	38.0	41.0	34.0	41.0
18	38.433519296202064	40.0	38.0	41.0	34.0	41.0
19	38.37678938172455	40.0	37.0	41.0	34.0	41.0
20	38.24677068326027	40.0	37.0	41.0	34.0	41.0
21	38.18714863746437	40.0	37.0	41.0	34.0	41.0
22	38.16667075376268	40.0	37.0	41.0	34.0	41.0
23	38.12115991784937	40.0	37.0	41.0	34.0	41.0
24	38.099077338074366	40.0	37.0	41.0	34.0	41.0
25	38.068431781258624	40.0	37.0	41.0	34.0	41.0
26	37.93688042178831	40.0	36.0	41.0	34.0	41.0
27	37.88237593109156	40.0	36.0	41.0	33.0	41.0
28	37.79283021181375	40.0	36.0	41.0	33.0	41.0
29	37.742992673880394	40.0	36.0	41.0	33.0	41.0
30	37.73139196272568	40.0	36.0	41.0	33.0	41.0
31	37.636383839622354	40.0	36.0	41.0	33.0	41.0
32	37.436135548539376	40.0	36.0	41.0	33.0	41.0
33	37.32750973239739	40.0	36.0	41.0	32.0	41.0
34	37.14098028997946	40.0	35.0	41.0	32.0	41.0
35	37.08542592649358	40.0	35.0	41.0	31.0	41.0
36	36.95997149250529	39.0	35.0	41.0	31.0	41.0
37	36.91075621494038	39.0	35.0	41.0	31.0	41.0
38	36.79890568004169	39.0	35.0	41.0	31.0	41.0
39	36.74146154553536	39.0	35.0	41.0	31.0	41.0
40	36.64815927413175	39.0	35.0	41.0	31.0	41.0
41	36.52225730312969	39.0	35.0	41.0	30.0	41.0
42	36.44681513042945	39.0	35.0	41.0	30.0	41.0
43	36.394721515495206	39.0	35.0	41.0	30.0	41.0
44	36.24689023081875	39.0	35.0	41.0	30.0	41.0
45	36.176585537810745	39.0	35.0	41.0	30.0	41.0
46	36.0640851546455	38.0	35.0	40.0	30.0	41.0
47	35.96496183674095	38.0	35.0	40.0	29.0	41.0
48	35.82598780001839	38.0	35.0	40.0	29.0	41.0
49	35.74739754161175	38.0	35.0	40.0	29.0	41.0
50	35.651624620666404	38.0	35.0	40.0	28.0	41.0
51	34.35679428623977	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	5.0
13	11.0
14	8.0
15	16.0
16	31.0
17	59.0
18	155.0
19	244.0
20	415.0
21	736.0
22	1148.0
23	1782.0
24	2783.0
25	4043.0
26	5599.0
27	6719.0
28	7472.0
29	8425.0
30	10040.0
31	12562.0
32	16290.0
33	22063.0
34	36917.0
35	50497.0
36	49363.0
37	72402.0
38	126858.0
39	215651.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.069000398491866	21.355945192042427	24.397051160224382	11.178003249241334
2	33.00110351592435	24.875241394108453	24.57361370812004	17.550041381847162
3	31.76424608405113	24.538209238880544	27.213775557122275	16.48376911994605
4	28.302884468013367	25.90028507494712	26.887625295037243	18.90920516200227
5	25.59620513134905	30.76633050301934	24.45927719706955	19.178187168562058
6	26.440241547374555	34.73684210526316	23.632100052110474	15.190816295251816
7	79.7662691965791	6.264598596082519	9.038715017012537	4.930417190325843
8	79.9206081598872	5.7270943812647515	9.07488581675505	5.277411642093002
9	72.98516384146154	8.303650798516383	11.55473132452564	7.156454035496429
10	37.54973484964596	29.730864727339608	17.59939306624161	15.12000735677283
11	29.345400484320876	24.834012813046012	26.11133249547865	19.709254207154462
12	26.345369831100758	23.137970143763603	29.666952763387794	20.849707261747845
13	24.36777733500904	24.79492995739202	31.68623363884376	19.15105906875517
14	21.734359194433374	27.31048646660332	29.43889280568924	21.51626153327407
15	20.468534469546025	25.6260920209668	33.557612727217	20.34776078227018
16	22.962633724672777	25.598197590656895	30.86166201759495	20.577506667075376
17	23.081108420439566	25.44692394936088	28.02577935812157	23.44618827207798
18	24.370229592618706	24.697452717407963	29.793244030285383	21.139073659687952
19	23.915489072127027	27.099745578272998	27.09897924776998	21.885786101829996
20	25.725255188057506	26.1013701989394	27.67280752843086	20.500567084572236
21	23.897097140054562	27.67158139962603	27.739631548294145	20.691689912025257
22	23.641602550347915	24.99080403396377	26.95215032339147	24.415443092296847
23	21.956595040308986	26.82570579039328	27.8128927443828	23.404806424914938
24	22.686294945284004	24.99524875088128	29.172516322839716	23.145939980995003
25	22.58912423750115	27.113079729025536	27.05437881249425	23.243417220979065
26	22.215768016430125	26.557949912638325	28.385341630138246	22.840940440793307
27	23.567115225454437	26.459706342151247	27.75833001256782	22.214848419826502
28	20.612298071912456	26.800110351592433	28.577537320295498	24.010054256199613
29	22.0799742512951	25.24522576096619	28.339821598258897	24.334978389479815
30	24.291297550807712	25.25825337951752	27.18511479630935	23.26533427336542
31	24.58740765717439	26.687613033749198	24.613462894277045	24.111516414799375
32	22.88937252858413	27.69947582993593	26.47579928271465	22.935352358765286
33	23.54243938325721	26.46874904208687	25.396192870061	24.59261870459492
34	21.32529197192165	26.01906630291512	27.847684149219877	24.807957575943355
35	22.35309444257119	26.852833890200166	27.108175213806213	23.68589645342243
36	23.069153664592466	27.328725132575176	26.502007785917908	23.100113416914446
37	22.92079207920792	27.79204855470067	26.82723845139932	22.459920914692088
38	20.739049137111852	29.421420470220394	25.73996873371548	24.099561658952272
39	22.790515893694632	25.288600067437084	27.91696042669282	24.00392361217546
40	23.787358612022196	25.023296447291788	28.41997976887472	22.769365171811298
41	21.835668086932532	25.80541335867333	27.45884805198786	24.900070502406276
42	22.299604573460442	25.66088342580388	28.787205345921592	23.252306654814088
43	22.631272415167214	24.418814946510132	28.942770438034515	24.00714220028814
44	22.56812678171842	25.169512307267876	26.56576648376912	25.696594427244584
45	22.80231738344113	24.193513778622446	26.86647457315391	26.137694264782514
46	23.410630536737884	25.308064862213776	26.629525181620327	24.65177941942801
47	21.425221469515375	25.354044692394933	29.35781503846979	23.8629187996199
48	21.619409618980473	24.304325169359043	28.845752996352264	25.23051221530822
49	22.453790270667934	23.553934340802503	29.459736995371365	24.5325383931582
50	21.039757226496643	24.12531036385372	29.4137571651902	25.42117524445943
51	21.438862152469117	23.221653434693316	27.18879318272385	28.150691230113722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	254.0
1	549.0
2	844.0
3	2758.5
4	4673.0
5	3113.5
6	1554.0
7	1543.0
8	1532.0
9	1517.0
10	1502.0
11	1611.0
12	1720.0
13	1743.5
14	1767.0
15	1723.0
16	1679.0
17	1640.5
18	1602.0
19	1645.5
20	1689.0
21	1850.0
22	2011.0
23	2219.0
24	2427.0
25	2740.5
26	3618.0
27	4182.0
28	5033.0
29	5884.0
30	7265.0
31	8646.0
32	9691.0
33	10736.0
34	12821.0
35	14906.0
36	15878.0
37	16850.0
38	18591.5
39	20333.0
40	23399.0
41	26465.0
42	29880.5
43	33296.0
44	37601.5
45	41907.0
46	50304.5
47	58702.0
48	64723.5
49	70745.0
50	69450.0
51	68155.0
52	61160.0
53	54165.0
54	47899.0
55	41633.0
56	37689.0
57	33745.0
58	30617.0
59	27489.0
60	25770.5
61	24052.0
62	21757.0
63	19462.0
64	17315.5
65	15169.0
66	12897.0
67	10625.0
68	8882.0
69	7139.0
70	5814.5
71	4490.0
72	3872.5
73	3255.0
74	2492.0
75	1514.0
76	1299.0
77	896.0
78	493.0
79	414.0
80	335.0
81	223.5
82	112.0
83	98.0
84	84.0
85	56.5
86	29.0
87	27.0
88	25.0
89	14.5
90	4.0
91	4.0
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	652460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.59520748755858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2785877407853	26.083665350285195
2	10.042613897290124	7.149378507829626
3	3.65079678828311	3.898526075215494
4	1.9638583121153124	2.796157763836445
5	1.2958660043368537	2.30633096502219
6	0.9685096328249234	2.0684580800461396
7	0.7919101676482047	1.9731744710261603
8	0.6987494782799295	1.9897706128957922
9	0.5882106542076196	1.8843732254621512
>10	6.595777535504604	43.68777222868213
>50	0.07966104197215079	1.9167710480861548
>100	0.041562282744540435	2.69115394741818
>500	0.002164702226278148	0.47156451234608204
>1k	0.001731761781022518	1.0829032118483395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2919	0.44738374766269196	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1957	0.29994175888177055	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1097	0.16813291236244368	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	1054	0.16154247003647734	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	738	0.11311038224565491	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17993440210894154	0.0
2	0.0	0.0	0.0	0.8699383870275572	0.0
3	0.0	0.0	0.0	1.2580081537565522	0.0
4	0.0	0.0	0.0	1.7220979063850657	0.0
5	0.0	0.0	0.0	3.1462465131962114	0.0
6	0.0	0.0	0.0	4.172822855040922	0.0
7	0.0	0.0	0.0	5.153266100603869	0.0
8	0.0	0.0	0.0	6.824632927689054	0.0
9	0.0	0.0	0.0	7.575023756245594	0.0
10	0.0	0.0	0.0	8.951200073567728	0.0
11	0.0	0.0	0.0	10.560953928210159	0.0
12	0.0	0.0	0.0	11.851914293596542	0.0
13	0.0	0.0	0.0	12.40765717438617	0.0
14	0.0	0.0	0.0	12.654262330257794	0.0
15	0.0	0.0	0.0	13.069460196793672	0.0
16	0.0	0.0	0.0	13.830273120191276	0.0
17	0.0	0.0	0.0	14.792630965882966	0.0
18	0.0	0.0	0.0	15.783956104588787	0.0
19	0.0	0.0	0.0	16.394721515495203	0.0
20	0.0	0.0	0.0	17.00594672470343	0.0
21	0.0	0.0	0.0	17.72461147043497	0.0
22	0.0	0.0	0.0	18.485884192134385	0.0
23	0.0	0.0	0.0	19.282101584771482	0.0
24	0.0	0.0	0.0	19.928577997118598	0.0
25	0.0	0.0	0.0	20.50914998620605	0.0
26	0.0	0.0	0.0	21.033320050271282	0.0
27	0.0	0.0	0.0	21.54277656867854	0.0
28	0.0	0.0	0.0	22.070625019158264	0.0
29	0.0	0.0	0.0	22.64460656591975	0.0
30	0.0	0.0	0.0	23.336603010146217	0.0
31	0.0	0.0	0.0	23.901695123072678	0.0
32	1.5326610060386844E-4	0.0	0.0	24.46433497838948	0.0
33	1.5326610060386844E-4	0.0	0.0	24.983293995034177	0.0
34	1.5326610060386844E-4	0.0	0.0	25.519572081047112	0.0
35	1.5326610060386844E-4	0.0	0.0	26.10704104466174	0.0
36	1.5326610060386844E-4	0.0	0.0	26.66171106274714	0.0
37	1.5326610060386844E-4	0.0	0.0	27.210097170707783	0.0
38	3.0653220120773687E-4	0.0	0.0	27.76660638200043	0.0
39	3.0653220120773687E-4	0.0	0.0	28.345339177880636	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.031042E-4	45.0	2
CGACGGT	25	3.8889455E-5	45.0	28
TCTACGG	45	3.8380676E-10	45.0	2
TTACGCG	20	7.031042E-4	45.0	1
ACGTATA	25	3.8889455E-5	45.0	15
TCGACAT	20	7.031042E-4	45.0	19
GACCTAT	90	0.0	42.5	33
CACAACG	255	0.0	41.47059	12
CAACGAG	255	0.0	40.588234	14
ACCTATG	95	0.0	40.26316	34
ACAACGA	260	0.0	39.80769	13
AACGAGC	260	0.0	39.80769	15
AATGCGG	85	0.0	39.705883	2
TCGATGG	40	3.4551522E-7	39.375	2
TGATTCG	40	3.4551522E-7	39.375	15
CGAATAT	105	0.0	38.57143	14
ATTAGCG	35	6.2441395E-6	38.571426	1
GAGTACG	35	6.2441395E-6	38.571426	1
ATCGTAG	35	6.2441395E-6	38.571426	1
TATAGCG	35	6.2441395E-6	38.571426	1
>>END_MODULE