FastQCFastQC Report
Sat 18 Jun 2016
SRR3550464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998742
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52020.5208552358867455No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC17320.17341816004533703No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC15920.1594005258615338No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT15270.1528923385619109No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG12730.1274603451141536No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGT207.032843E-445.014
CGTTTTT28950.041.191711
AATTGCG501.0804797E-940.5000041
CTACGAA1000.040.50000411
GCGTTAG850.039.7058831
GCGTAAG1150.039.1304361
TCGTGCG356.2474683E-638.5714261
ACGTACG356.2474683E-638.5714261
GTACGAG900.037.51
GTCGACG301.1398147E-437.4999961
CTATACG301.1398147E-437.4999961
CTGTTCG301.1398147E-437.4999961
TACGAAT1100.036.8181812
TACGGGT800.036.56254
GACCCGT250.002106976236.0000049
TCGGACT250.002106976236.00000414
TTCGGAC250.002106976236.00000413
TAAGGCG250.002106976236.0000041
CGGTCTA504.877802E-836.00000431
CGAGGGA7550.035.761594
Produced by FastQC (version 0.11.5)