##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	998742
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.266432171671966	33.0	31.0	34.0	30.0	34.0
2	32.380984278222	34.0	31.0	34.0	30.0	34.0
3	32.46950563809272	34.0	31.0	34.0	30.0	34.0
4	35.97478127484376	37.0	35.0	37.0	35.0	37.0
5	35.944691421808635	37.0	35.0	37.0	35.0	37.0
6	35.96182697833875	37.0	35.0	37.0	35.0	37.0
7	36.14178035969249	37.0	35.0	37.0	35.0	37.0
8	36.143187129408794	37.0	36.0	37.0	35.0	37.0
9	37.723433078813144	39.0	38.0	39.0	35.0	39.0
10	37.442540716221	39.0	37.0	39.0	35.0	39.0
11	37.404248544669194	39.0	37.0	39.0	35.0	39.0
12	37.35547418652665	39.0	37.0	39.0	35.0	39.0
13	37.3276882317956	39.0	37.0	39.0	34.0	39.0
14	38.54995284067357	40.0	38.0	41.0	34.0	41.0
15	38.60204437181975	40.0	38.0	41.0	34.0	41.0
16	38.61757991553374	40.0	38.0	41.0	35.0	41.0
17	38.62463779434529	40.0	38.0	41.0	35.0	41.0
18	38.56437197995078	40.0	38.0	41.0	34.0	41.0
19	38.509160523939116	40.0	38.0	41.0	34.0	41.0
20	38.39967979718486	40.0	37.0	41.0	34.0	41.0
21	38.34718876346444	40.0	37.0	41.0	34.0	41.0
22	38.33656940431062	40.0	37.0	41.0	34.0	41.0
23	38.29719887618624	40.0	37.0	41.0	34.0	41.0
24	38.28428162628587	40.0	37.0	41.0	34.0	41.0
25	38.24581924060468	40.0	37.0	41.0	34.0	41.0
26	38.13767619665539	40.0	37.0	41.0	34.0	41.0
27	38.07959513067439	40.0	37.0	41.0	34.0	41.0
28	37.99795242414958	40.0	37.0	41.0	34.0	41.0
29	37.95124566704915	40.0	37.0	41.0	34.0	41.0
30	37.921692489151354	40.0	37.0	41.0	33.0	41.0
31	37.79808398965899	40.0	36.0	41.0	33.0	41.0
32	37.61932811476838	40.0	36.0	41.0	33.0	41.0
33	37.48892606899479	40.0	36.0	41.0	33.0	41.0
34	37.31070586798192	40.0	36.0	41.0	32.0	41.0
35	37.28740855996844	40.0	36.0	41.0	32.0	41.0
36	37.14561918893968	40.0	36.0	41.0	32.0	41.0
37	37.10738008414585	40.0	35.0	41.0	32.0	41.0
38	37.02324824629384	40.0	35.0	41.0	31.0	41.0
39	36.94087061523396	39.0	35.0	41.0	31.0	41.0
40	36.869797204883746	39.0	35.0	41.0	31.0	41.0
41	36.74748834033214	39.0	35.0	41.0	31.0	41.0
42	36.66400431743133	39.0	35.0	41.0	31.0	41.0
43	36.586585925093765	39.0	35.0	41.0	31.0	41.0
44	36.40670463443011	39.0	35.0	41.0	30.0	41.0
45	36.3291340506357	39.0	35.0	41.0	30.0	41.0
46	36.22612346331685	39.0	35.0	41.0	30.0	41.0
47	36.12072787566759	39.0	35.0	41.0	29.0	41.0
48	36.004175252467604	38.0	35.0	40.0	29.0	41.0
49	35.916197576551305	38.0	35.0	40.0	29.0	41.0
50	35.8193887910992	38.0	35.0	40.0	29.0	41.0
51	34.62954897260754	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	6.0
12	3.0
13	9.0
14	10.0
15	17.0
16	45.0
17	89.0
18	184.0
19	304.0
20	613.0
21	975.0
22	1494.0
23	2350.0
24	3723.0
25	5718.0
26	8177.0
27	10290.0
28	11153.0
29	12891.0
30	15108.0
31	18491.0
32	23229.0
33	30804.0
34	52545.0
35	71188.0
36	71318.0
37	105702.0
38	193060.0
39	358955.0
40	284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.429560386966806	21.61849606805361	25.211315835320836	12.74062770965875
2	32.016076223889655	24.14357261434885	25.17266721535692	18.667683946404576
3	31.0134148759139	24.378067609052188	27.916018351085665	16.692499163948245
4	27.886180815465856	25.729868174163094	27.39546349307429	18.98848751729676
5	25.229939263593597	30.289203818403553	25.15164076408121	19.32921615392163
6	26.10654202987358	33.986454960340104	24.488806919104235	15.418196090682079
7	81.65191811298614	5.477090179445742	8.4099797545312	4.4610119530369206
8	83.15591013494976	4.352074910237079	8.240266254948725	4.25174869986443
9	76.06779328395122	7.300884512716999	10.672025407963218	5.959296795368574
10	35.74596842828278	32.77032506893672	17.098710177403174	14.384996325377324
11	26.633104445392302	24.738420933534385	28.911971259844886	19.716503361228423
12	24.794191092394232	23.22772047235422	31.45947602083421	20.518612414417337
13	23.386019612672744	24.229680938620785	32.61643147079025	19.767867977916218
14	20.541240881028333	26.92957740837974	30.991387165053634	21.53779454553829
15	20.290825858930532	25.55144371619497	33.696690436569206	20.461039988305288
16	23.40884833120065	25.394946843128658	31.082101283414538	20.11410354225616
17	22.207236703773347	25.48596133936492	29.598735208892784	22.708066747968942
18	23.490050483508256	24.37586483796616	30.491157876608778	21.64292680191681
19	22.787967262816625	26.835559133389804	28.53810093097116	21.83837267282241
20	24.8058057035751	26.033049576367066	28.465008981298475	20.69613573875936
21	23.618211710331597	27.22304659261351	28.895250224782774	20.26349147227212
22	23.077131030836792	24.289856639652683	28.17634584307058	24.45666648643994
23	21.584052738344838	26.934683832260987	28.79322187311638	22.688041556277796
24	22.11512082199407	25.11129000282355	30.408754212799703	22.364834962382677
25	22.137849414563522	26.909151712854772	28.25915001071348	22.69384886186823
26	21.929887798850956	27.36862973620815	28.34555871286078	22.355923752080116
27	22.471569234096496	26.408121416742265	28.436372957180133	22.683936391981113
28	20.293228881933473	27.621247529391972	29.273726347745466	22.81179724092909
29	22.304358883475413	26.486720294130016	27.837519599656368	23.371401222738207
30	23.68319345736937	25.5475388038152	28.252942201289223	22.516325537526207
31	23.188671348556483	27.893289758516215	25.811270578387614	23.106768314539693
32	22.73219710395678	28.48503417298962	26.22128637826386	22.561482344789745
33	21.305001692128698	28.921783603773548	25.80896768134313	23.964247022754627
34	20.73908977493687	28.037671390609386	27.31556297822661	23.907675856227133
35	20.826299484751818	27.864853986314785	26.863594401757414	24.445252127175987
36	20.661492157133672	30.09255643599648	25.341379455354833	23.904571951515006
37	20.241964391204135	30.409354968550435	26.09532792252654	23.25335271771889
38	19.82634153765437	29.492301315054338	26.007016827168577	24.674340320122713
39	21.267254205790884	27.18449809860805	26.415330485751078	25.132917209849992
40	22.161879644592897	25.310440534192015	27.997621007227092	24.530058813988
41	20.173177857745042	26.83315611038687	26.805220967977718	26.188445063890377
42	21.506354994583184	28.185757683165424	26.75676000408514	23.551127318166255
43	21.83126372977205	27.15285829573604	27.718469835052495	23.297408139439415
44	21.612088006712444	27.191907419533774	27.045723520188396	24.150281053565383
45	21.446679923343567	25.231641404887352	27.60172296749311	25.719955704275982
46	22.271617695060385	25.816477128227312	26.851278908867354	25.06062626784495
47	20.432303838228492	26.634205830935315	28.374795492729852	24.558694838106337
48	20.43490711314834	26.592253054342365	28.013140530787734	24.959699301721567
49	21.38600359251939	25.814975238850472	28.41124134160774	24.387779827022392
50	19.703086482795356	25.343582226440866	29.02601472652597	25.927316564237813
51	20.15235165838625	24.48630377014284	27.89809580452209	27.463248766948823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	736.0
2	1119.0
3	4984.0
4	8849.0
5	5830.5
6	2812.0
7	2683.5
8	2555.0
9	2552.0
10	2549.0
11	2453.5
12	2358.0
13	2456.5
14	2555.0
15	2476.5
16	2398.0
17	2417.5
18	2437.0
19	2514.0
20	2591.0
21	2714.0
22	2837.0
23	3371.5
24	3906.0
25	4962.5
26	7090.5
27	8162.0
28	9915.5
29	11669.0
30	13959.5
31	16250.0
32	18475.5
33	20701.0
34	23692.0
35	26683.0
36	29363.0
37	32043.0
38	34942.0
39	37841.0
40	41862.0
41	45883.0
42	49564.0
43	53245.0
44	60113.5
45	66982.0
46	75619.5
47	84257.0
48	93044.5
49	101832.0
50	99930.5
51	98029.0
52	87883.5
53	77738.0
54	69237.5
55	60737.0
56	54849.5
57	48962.0
58	44508.0
59	40054.0
60	37870.5
61	35687.0
62	32626.0
63	29565.0
64	25782.5
65	22000.0
66	17650.5
67	13301.0
68	11012.0
69	8723.0
70	7226.5
71	5730.0
72	4536.5
73	3343.0
74	3075.0
75	2263.5
76	1720.0
77	1256.5
78	793.0
79	563.5
80	334.0
81	261.5
82	189.0
83	133.0
84	77.0
85	56.0
86	35.0
87	22.5
88	10.0
89	8.0
90	6.0
91	5.5
92	5.0
93	5.0
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	998742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.805384856858854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70509917000051	22.806082753010966
2	9.643775234352846	6.134479656032729
3	3.5054476581919083	3.3447633559310477
4	1.9357911728962194	2.4627433302589785
5	1.30353327910107	2.072968880776637
6	1.0538078823961055	2.011005915887975
7	0.9163659658541765	2.0401760549603485
8	0.8266353165611825	2.1033163503599805
9	0.73492329831781	2.103706650894303
>10	8.25164930482128	49.4299169230632
>50	0.08081915474968959	1.6859341175095306
>100	0.038349324012371296	2.1606092154694907
>500	0.0022185559345359995	0.5026032260962667
>1k	0.0012677462483062854	0.6173169186950536
>5k	3.1693656207657135E-4	0.5243766510535057
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5202	0.5208552358867455	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1732	0.17341816004533703	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1592	0.1594005258615338	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	1527	0.1528923385619109	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	1273	0.1274603451141536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.006297922786866E-4	0.0	0.0	0.16811148424718295	0.0
2	5.006297922786866E-4	0.0	0.0	0.6526209972144958	0.0
3	5.006297922786866E-4	0.0	0.0	1.0046638671448682	0.0
4	5.006297922786866E-4	0.0	0.0	1.343189732683716	0.0
5	5.006297922786866E-4	0.0	0.0	2.3732855932763415	0.0
6	5.006297922786866E-4	0.0	0.0	3.298649701324266	0.0
7	5.006297922786866E-4	0.0	0.0	4.1401082561862825	0.0
8	5.006297922786866E-4	0.0	0.0	5.531959204679487	0.0
9	5.006297922786866E-4	0.0	0.0	6.178672770345094	0.0
10	5.006297922786866E-4	0.0	0.0	7.240108055934366	0.0
11	5.006297922786866E-4	0.0	0.0	8.49338467792483	0.0
12	5.006297922786866E-4	0.0	0.0	9.542304218707134	0.0
13	5.006297922786866E-4	0.0	0.0	10.003284131437349	0.0
14	5.006297922786866E-4	0.0	0.0	10.221258342995489	0.0
15	5.006297922786866E-4	0.0	0.0	10.543563803264506	0.0
16	5.006297922786866E-4	0.0	0.0	11.16264260439633	0.0
17	5.006297922786866E-4	0.0	0.0	11.936315885383813	0.0
18	5.006297922786866E-4	0.0	0.0	12.738925568365003	0.0
19	5.006297922786866E-4	0.0	0.0	13.239154856809867	0.0
20	6.007557507344239E-4	0.0	0.0	13.72977205324298	0.0
21	6.007557507344239E-4	0.0	0.0	14.302092031775974	0.0
22	6.007557507344239E-4	0.0	0.0	14.955514036658116	0.0
23	6.007557507344239E-4	0.0	0.0	15.613041205836943	0.0
24	7.008817091901612E-4	0.0	0.0	16.1472131941983	0.0
25	7.008817091901612E-4	0.0	0.0	16.62641603136746	0.0
26	7.008817091901612E-4	0.0	0.0	17.06026180935617	0.0
27	7.008817091901612E-4	0.0	0.0	17.494107587344878	0.0
28	7.008817091901612E-4	0.0	0.0	17.959993672039424	0.0
29	8.010076676458986E-4	0.0	0.0	18.442000036045346	0.0
30	8.010076676458986E-4	0.0	0.0	19.023831980631634	0.0
31	8.010076676458986E-4	0.0	0.0	19.522759631616573	0.0
32	8.010076676458986E-4	0.0	0.0	20.008470656085354	0.0
33	8.010076676458986E-4	0.0	0.0	20.49478243630487	0.0
34	8.010076676458986E-4	0.0	0.0	20.937839802471508	0.0
35	8.010076676458986E-4	0.0	0.0	21.42755586527852	0.0
36	9.011336261016358E-4	0.0	0.0	21.886132755005796	0.0
37	9.011336261016358E-4	0.0	0.0	22.37234440926686	0.0
38	9.011336261016358E-4	0.0	0.0	22.846140444679406	0.0
39	9.011336261016358E-4	0.0	0.0	23.323541014596362	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGGT	20	7.032843E-4	45.0	14
CGTTTTT	2895	0.0	41.19171	1
AATTGCG	50	1.0804797E-9	40.500004	1
CTACGAA	100	0.0	40.500004	11
GCGTTAG	85	0.0	39.705883	1
GCGTAAG	115	0.0	39.130436	1
TCGTGCG	35	6.2474683E-6	38.571426	1
ACGTACG	35	6.2474683E-6	38.571426	1
GTACGAG	90	0.0	37.5	1
GTCGACG	30	1.1398147E-4	37.499996	1
CTATACG	30	1.1398147E-4	37.499996	1
CTGTTCG	30	1.1398147E-4	37.499996	1
TACGAAT	110	0.0	36.81818	12
TACGGGT	80	0.0	36.5625	4
GACCCGT	25	0.0021069762	36.000004	9
TCGGACT	25	0.0021069762	36.000004	14
TTCGGAC	25	0.0021069762	36.000004	13
TAAGGCG	25	0.0021069762	36.000004	1
CGGTCTA	50	4.877802E-8	36.000004	31
CGAGGGA	755	0.0	35.76159	4
>>END_MODULE