FastQCFastQC Report
Sat 18 Jun 2016
SRR3550463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972978
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128171.3172959717485904No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTT21990.22600716562964424No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG17030.17502965123569084No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC14830.1524186569480502No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT13920.14306592749270794No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC13090.1345354160114617No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT12260.12600490453021548No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC11590.11911882899716128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG302.1651558E-645.0000041
TATACGG351.2116652E-745.0000042
CGTTTTT60100.043.128121
TACGGGA3550.041.1971864
GCGTAAG950.040.263161
ACGATAG451.927765E-840.01
CGGGATA2250.038.06
TAACGCG301.13979295E-437.5000041
TATAGCG301.13979295E-437.5000041
TAGGACG301.13979295E-437.5000041
TCACGAC552.748493E-936.8181825
TAACGGG3100.036.290323
AGATACG250.002106950236.01
ATGTACG504.87762E-836.01
CGAATCC250.002106950236.028
CAATGCG250.002106950236.01
GCGGAGT250.002106950236.05
ATACGCA250.002106950236.010
GTTTTTT74550.035.885312
CTAACGG702.1827873E-1135.3571432