##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972978
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29126763400611	33.0	31.0	34.0	30.0	34.0
2	32.432437321296064	34.0	31.0	34.0	31.0	34.0
3	32.45575336749649	34.0	31.0	34.0	30.0	34.0
4	35.98892677943386	37.0	35.0	37.0	35.0	37.0
5	35.964712460096735	37.0	35.0	37.0	35.0	37.0
6	35.98683629023472	37.0	35.0	37.0	35.0	37.0
7	36.15916803874291	37.0	35.0	37.0	35.0	37.0
8	36.1512171909334	37.0	36.0	37.0	35.0	37.0
9	37.73151191496622	39.0	38.0	39.0	35.0	39.0
10	37.506183079165204	39.0	37.0	39.0	35.0	39.0
11	37.45368651706411	39.0	37.0	39.0	35.0	39.0
12	37.38506523271852	39.0	37.0	39.0	35.0	39.0
13	37.35673262910364	39.0	37.0	39.0	34.0	39.0
14	38.611766144763806	40.0	38.0	41.0	34.0	41.0
15	38.65782474012773	40.0	38.0	41.0	35.0	41.0
16	38.6721282495596	40.0	38.0	41.0	35.0	41.0
17	38.674243405297965	40.0	38.0	41.0	35.0	41.0
18	38.535651371356806	40.0	38.0	41.0	35.0	41.0
19	38.40592490272134	40.0	37.0	41.0	34.0	41.0
20	38.22200501964073	40.0	36.0	41.0	34.0	41.0
21	38.169194986937015	40.0	36.0	41.0	34.0	41.0
22	38.15004244700291	40.0	36.0	41.0	34.0	41.0
23	38.113824773016454	40.0	36.0	41.0	34.0	41.0
24	38.08797732322827	40.0	36.0	41.0	34.0	41.0
25	38.048026779639414	40.0	36.0	41.0	34.0	41.0
26	37.924446390360316	40.0	36.0	41.0	34.0	41.0
27	37.86540600095788	40.0	36.0	41.0	34.0	41.0
28	37.75661731303277	40.0	36.0	41.0	33.0	41.0
29	37.69568890560732	40.0	35.0	41.0	33.0	41.0
30	37.63929605808148	40.0	35.0	41.0	33.0	41.0
31	37.486256626562984	40.0	35.0	41.0	33.0	41.0
32	37.231747274861306	40.0	35.0	41.0	32.0	41.0
33	36.98796581217664	40.0	35.0	41.0	31.0	41.0
34	36.68080162141384	40.0	35.0	41.0	30.0	41.0
35	36.56923486450876	39.0	35.0	41.0	30.0	41.0
36	36.41382127859006	39.0	35.0	41.0	30.0	41.0
37	36.34049485188771	39.0	35.0	41.0	30.0	41.0
38	36.25518459821291	39.0	35.0	41.0	30.0	41.0
39	36.14986155904861	39.0	35.0	41.0	29.0	41.0
40	36.070130054328054	39.0	35.0	41.0	29.0	41.0
41	35.932873096822334	39.0	35.0	41.0	28.0	41.0
42	35.8733979596661	39.0	35.0	41.0	28.0	41.0
43	35.81788077428266	39.0	35.0	41.0	28.0	41.0
44	35.65026444585592	39.0	35.0	41.0	27.0	41.0
45	35.59283765922765	39.0	35.0	41.0	27.0	41.0
46	35.500547802725244	38.0	35.0	41.0	26.0	41.0
47	35.408968136997956	38.0	35.0	40.0	26.0	41.0
48	35.28918845030412	38.0	35.0	40.0	26.0	41.0
49	35.217292682876696	38.0	35.0	40.0	26.0	41.0
50	35.14128068671645	38.0	34.0	40.0	25.0	41.0
51	33.965255123959636	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	9.0
12	10.0
13	10.0
14	22.0
15	27.0
16	59.0
17	111.0
18	220.0
19	403.0
20	749.0
21	1300.0
22	2027.0
23	3066.0
24	5005.0
25	8275.0
26	12526.0
27	14892.0
28	15581.0
29	15391.0
30	16647.0
31	18995.0
32	23148.0
33	30218.0
34	52089.0
35	68996.0
36	70554.0
37	98829.0
38	177641.0
39	335940.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.55503413232365	20.85483947221828	24.11842816589892	16.47169822955915
2	36.44244782513068	22.86577908236363	23.99571213326509	16.6960609592406
3	30.57026983138365	23.232077189823407	30.266665844448692	15.930987134344251
4	27.42919161584332	24.66643644563392	30.065325218041927	17.839046720480834
5	24.870243725962972	28.858720341056017	27.934444560925325	18.33659137205569
6	25.858446953579627	32.5487318315522	26.600807006941572	14.992014207926593
7	77.45807202218344	5.569087893045886	12.44920234578788	4.523637738982793
8	78.56518852430374	4.823850076774603	12.382191580899054	4.228769818022607
9	71.73533214522836	7.2809457151138055	14.743704379749595	6.24001775990824
10	36.23319335072324	28.819151101052643	20.667168219630867	14.280487328593248
11	26.92568588395627	23.166710860882773	30.950648421649824	18.956954833511137
12	24.46694580966887	22.38745377593327	32.988412893200056	20.157187521197805
13	23.075341888511353	22.424042475780542	34.83264780909743	19.667967826610674
14	20.1793874064984	24.999126393402523	33.81803082906294	21.00345537103614
15	19.409483051004237	24.70641679462434	35.936269884827816	19.947830269543605
16	22.691571649102034	24.442895933926565	33.668181603283934	19.197350813687464
17	22.184879822565364	24.504459504737003	31.844399359492197	21.466261313205436
18	22.91243995239358	23.886973806190888	32.607828748440355	20.592757492975174
19	22.584888866963077	25.64333417610676	30.71508297207131	21.056693984858857
20	24.27526624445774	24.842288314843707	30.822485194937606	20.05996024576095
21	22.99034510543918	26.155473196721818	31.05877008524345	19.795411612595558
22	22.390228761595843	23.90547371060805	30.94509845032467	22.759199077471433
23	21.59976895674928	25.468818411104877	31.071720018335462	21.859692613810385
24	21.425150414500635	23.94884570874162	32.73599197515257	21.890011901605174
25	21.84067882315941	25.87684408075003	30.10222224962949	22.18025484646107
26	21.409322718499286	26.1822980581267	30.608194635438828	21.800184587935185
27	22.147777236484277	24.907962975524626	30.938931815518956	22.00532797247214
28	20.61403238305491	25.578995619633744	31.56392025307869	22.243051744232655
29	22.126091237417494	24.127369786367215	30.4127123120975	23.33382666411779
30	22.78437950292812	23.185210765299935	31.56977855614413	22.460631175627814
31	22.321779115252347	24.557389786819435	30.764107718776785	22.35672337915143
32	22.713052093675294	24.293766148874898	30.795968665273005	22.197213092176803
33	22.88797896766422	24.819677320556067	29.990811714139475	22.301531997640232
34	21.7528042771779	24.740333286055797	31.45826524340735	22.048597193358948
35	22.33257072616236	24.213497119153775	30.4722203379727	22.98171181671117
36	21.96616984145582	26.694642633235283	29.395114791906906	21.94407273340199
37	22.31150139057615	26.621156901800454	30.31826002232322	20.74908168530018
38	21.923928393036636	26.474493770671074	29.345062272733813	22.256515563558477
39	22.997025626478706	25.499857139627004	28.96889754958488	22.53421968430941
40	23.017786630324633	24.34361311355447	30.648791647909817	21.98980860821108
41	19.96180797510324	25.975407460394788	29.925342607952082	24.13744195654989
42	21.35043135610466	26.223717288571784	29.598716517742435	22.827134837581116
43	22.35312617551476	24.52121219595921	30.567186513980786	22.558475114545242
44	22.652105186345427	25.076620437461074	29.38504262172423	22.886231754469268
45	22.345212327514087	23.62468627245426	29.614955322730836	24.41514607730082
46	22.228046266205403	24.574348032535166	29.188224194174992	24.009381507084434
47	21.499869472896613	24.725841694262357	31.041297953293906	22.73299087954712
48	21.133571365436836	23.90763203279005	30.93625960710314	24.02253699466997
49	21.659790868858288	23.906809814815958	30.787746485532047	23.645652830793708
50	20.366030886618198	23.671141587990686	31.861974268688503	24.100853256702617
51	20.55801878356962	23.136391573087984	30.53830610764067	25.767283535701736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	328.0
1	758.0
2	1188.0
3	10925.5
4	20663.0
5	13433.0
6	6203.0
7	5928.0
8	5653.0
9	5573.5
10	5494.0
11	5406.0
12	5318.0
13	5067.5
14	4817.0
15	4639.0
16	4461.0
17	4136.0
18	3811.0
19	3859.0
20	3907.0
21	4108.5
22	4310.0
23	4417.5
24	4525.0
25	5378.5
26	7453.5
27	8675.0
28	10204.0
29	11733.0
30	13676.0
31	15619.0
32	17627.5
33	19636.0
34	21761.0
35	23886.0
36	26709.0
37	29532.0
38	31736.0
39	33940.0
40	37492.0
41	41044.0
42	44169.5
43	47295.0
44	52649.5
45	58004.0
46	67687.5
47	77371.0
48	83292.5
49	89214.0
50	88276.0
51	87338.0
52	78173.0
53	69008.0
54	62295.0
55	55582.0
56	51007.0
57	46432.0
58	44536.0
59	42640.0
60	40877.5
61	39115.0
62	35467.0
63	31819.0
64	27366.5
65	22914.0
66	19410.5
67	15907.0
68	13469.0
69	11031.0
70	8879.5
71	6728.0
72	5741.0
73	4754.0
74	3925.5
75	2516.5
76	1936.0
77	1419.5
78	903.0
79	647.5
80	392.0
81	331.5
82	271.0
83	200.5
84	130.0
85	91.5
86	53.0
87	44.0
88	35.0
89	26.0
90	17.0
91	15.0
92	13.0
93	8.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	972978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.047237500719014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83699997813451	24.458513995946923
2	8.569983745168454	5.835685438981035
3	3.3660636889870705	3.438155095844676
4	2.1589463021909934	2.940246300079833
5	1.551752514592531	2.6416443203334925
6	1.3592033598058246	2.7766271761850496
7	1.2280078395242395	2.9267192195518636
8	1.0539960228216054	2.8708522331056368
9	1.0075545242731871	3.0874003364567844
>10	7.7729534186842155	42.773954605042306
>50	0.059276810532392636	1.3807647660129083
>100	0.02979013739681521	1.7951577358642015
>500	0.0030398099378640573	0.6640375919282062
>1k	0.00212786695650484	1.0837184577743528
>5k	0.0	0.0
>10k+	3.0398099378640573E-4	1.32652272689274
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12817	1.3172959717485904	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTT	2199	0.22600716562964424	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	1703	0.17502965123569084	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	1483	0.1524186569480502	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT	1392	0.14306592749270794	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1309	0.1345354160114617	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	1226	0.12600490453021548	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1159	0.11911882899716128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.16663480572017E-4	0.0	0.0	0.13926316936251384	0.0
2	6.16663480572017E-4	0.0	0.0	0.49672243360075974	0.0
3	6.16663480572017E-4	0.0	0.0	0.868878844125972	0.0
4	6.16663480572017E-4	0.0	0.0	1.2240770089354538	0.0
5	6.16663480572017E-4	0.0	0.0	2.233144017644798	0.0
6	6.16663480572017E-4	0.0	0.0	3.431218383149465	0.0
7	6.16663480572017E-4	0.0	0.0	4.383655128892945	0.0
8	6.16663480572017E-4	0.0	0.0	5.700539991654487	0.0
9	6.16663480572017E-4	0.0	0.0	6.449580566055964	0.0
10	6.16663480572017E-4	0.0	0.0	7.496777933314012	0.0
11	6.16663480572017E-4	0.0	0.0	8.627122093202518	0.0
12	6.16663480572017E-4	0.0	0.0	9.521078585538419	0.0
13	6.16663480572017E-4	0.0	0.0	9.971448480849515	0.0
14	7.194407273340198E-4	0.0	0.0	10.219141645545943	0.0
15	7.194407273340198E-4	0.0	0.0	10.506815159232788	0.0
16	7.194407273340198E-4	0.0	0.0	10.99182098670268	0.0
17	7.194407273340198E-4	0.0	0.0	11.565831909868466	0.0
18	8.222179740960227E-4	0.0	0.0	12.177150973608859	0.0
19	8.222179740960227E-4	0.0	0.0	12.578393344967719	0.0
20	9.249952208580255E-4	0.0	0.0	12.976552398923717	0.0
21	0.0010277724676200284	0.0	0.0	13.45241105143179	0.0
22	0.0011305497143820312	0.0	0.0	13.968866716410854	0.0
23	0.0011305497143820312	0.0	0.0	14.47668909266191	1.0277724676200284E-4
24	0.0011305497143820312	0.0	0.0	14.909895187763752	1.0277724676200284E-4
25	0.0011305497143820312	0.0	0.0	15.299009844004695	1.0277724676200284E-4
26	0.001233326961144034	0.0	0.0	15.6544135633077	1.0277724676200284E-4
27	0.001233326961144034	0.0	0.0	16.027906078040818	1.0277724676200284E-4
28	0.001233326961144034	0.0	0.0	16.410134658748706	1.0277724676200284E-4
29	0.0013361042079060369	0.0	0.0	16.810657589380234	1.0277724676200284E-4
30	0.0013361042079060369	0.0	0.0	17.301830051655845	1.0277724676200284E-4
31	0.0013361042079060369	0.0	0.0	17.716330687847	2.0555449352400567E-4
32	0.0013361042079060369	0.0	0.0	18.161458943573237	2.0555449352400567E-4
33	0.0013361042079060369	0.0	0.0	18.55365691721704	2.0555449352400567E-4
34	0.0013361042079060369	0.0	0.0	18.950685421458655	2.0555449352400567E-4
35	0.0013361042079060369	0.0	0.0	19.352441679051324	2.0555449352400567E-4
36	0.0013361042079060369	0.0	0.0	19.762009007397907	2.0555449352400567E-4
37	0.0013361042079060369	0.0	0.0	20.160579170340952	2.0555449352400567E-4
38	0.0013361042079060369	0.0	0.0	20.574154811311253	2.0555449352400567E-4
39	0.0013361042079060369	0.0	0.0	21.012499768751194	2.0555449352400567E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	30	2.1651558E-6	45.000004	1
TATACGG	35	1.2116652E-7	45.000004	2
CGTTTTT	6010	0.0	43.12812	1
TACGGGA	355	0.0	41.197186	4
GCGTAAG	95	0.0	40.26316	1
ACGATAG	45	1.927765E-8	40.0	1
CGGGATA	225	0.0	38.0	6
TAACGCG	30	1.13979295E-4	37.500004	1
TATAGCG	30	1.13979295E-4	37.500004	1
TAGGACG	30	1.13979295E-4	37.500004	1
TCACGAC	55	2.748493E-9	36.81818	25
TAACGGG	310	0.0	36.29032	3
AGATACG	25	0.0021069502	36.0	1
ATGTACG	50	4.87762E-8	36.0	1
CGAATCC	25	0.0021069502	36.0	28
CAATGCG	25	0.0021069502	36.0	1
GCGGAGT	25	0.0021069502	36.0	5
ATACGCA	25	0.0021069502	36.0	10
GTTTTTT	7455	0.0	35.88531	2
CTAACGG	70	2.1827873E-11	35.357143	2
>>END_MODULE