FastQCFastQC Report
Sat 18 Jun 2016
SRR3550461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823873
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28250.34289265457176044No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC16600.2014873651642911No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC16430.19942394034031946No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT11710.14213355699240052Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9520.11555179014241272No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG8570.10402088671433583No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGT207.032121E-445.00000445
CATTTCG207.032121E-445.00000430
AATACGC207.032121E-445.00000445
GTACGAG351.2113742E-745.01
CGAATAT1800.041.2514
TAGTGCG501.0804797E-940.51
TACGAAT1850.040.13513612
TATAGCG451.9272193E-840.01
GCGATAT403.4566256E-739.3750049
TTGACGT356.2461368E-638.57142619
CGCAATC356.2461368E-638.57142620
GCGTAAG356.2461368E-638.5714261
CGTTTTT14600.037.9109571
GTCGATA301.1396414E-437.4999969
TAGCCGT301.1396414E-437.49999644
TTTGCGC301.1396414E-437.49999613
TAAGCGA301.1396414E-437.49999624
GCGTATG301.1396414E-437.4999961
GAGTACG552.746674E-936.818181
CACAACG1900.036.71052612