##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550461_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	823873
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30545848692699	33.0	31.0	34.0	30.0	34.0
2	32.403001433473364	34.0	31.0	34.0	30.0	34.0
3	32.50272918277453	34.0	31.0	34.0	31.0	34.0
4	35.99795842320358	37.0	35.0	37.0	35.0	37.0
5	35.96827059510386	37.0	35.0	37.0	35.0	37.0
6	35.98562885299069	37.0	35.0	37.0	35.0	37.0
7	36.132244896968345	37.0	35.0	37.0	35.0	37.0
8	36.108879645285135	37.0	35.0	37.0	35.0	37.0
9	37.65535222054856	39.0	38.0	39.0	35.0	39.0
10	37.480867803654206	39.0	37.0	39.0	35.0	39.0
11	37.435948259015646	39.0	37.0	39.0	35.0	39.0
12	37.32357657066077	39.0	37.0	39.0	35.0	39.0
13	37.28017303637818	39.0	37.0	39.0	34.0	39.0
14	38.4930954164052	40.0	38.0	41.0	34.0	41.0
15	38.55100239964169	40.0	38.0	41.0	34.0	41.0
16	38.575234289750966	40.0	38.0	41.0	34.0	41.0
17	38.58877399793415	40.0	38.0	41.0	35.0	41.0
18	38.53001008650605	40.0	38.0	41.0	34.0	41.0
19	38.487450128842674	40.0	37.0	41.0	34.0	41.0
20	38.37136913092188	40.0	37.0	41.0	34.0	41.0
21	38.32042924091456	40.0	37.0	41.0	34.0	41.0
22	38.292960201390265	40.0	37.0	41.0	34.0	41.0
23	38.27475715310491	40.0	37.0	41.0	34.0	41.0
24	38.24504990453626	40.0	37.0	41.0	34.0	41.0
25	38.20822505410421	40.0	37.0	41.0	34.0	41.0
26	38.06001895923279	40.0	37.0	41.0	34.0	41.0
27	37.9769903856541	40.0	36.0	41.0	34.0	41.0
28	37.89612233924404	40.0	36.0	41.0	33.0	41.0
29	37.842526700110334	40.0	36.0	41.0	33.0	41.0
30	37.833153896292266	40.0	36.0	41.0	33.0	41.0
31	37.71339271950895	40.0	36.0	41.0	33.0	41.0
32	37.549595629423465	40.0	36.0	41.0	33.0	41.0
33	37.43021193800501	40.0	36.0	41.0	33.0	41.0
34	37.247905927248496	40.0	35.0	41.0	32.0	41.0
35	37.238602308851	40.0	35.0	41.0	32.0	41.0
36	37.0703421522492	39.0	35.0	41.0	31.0	41.0
37	37.0151079110494	39.0	35.0	41.0	31.0	41.0
38	36.93373614622642	39.0	35.0	41.0	31.0	41.0
39	36.83011823424241	39.0	35.0	41.0	31.0	41.0
40	36.769584632582934	39.0	35.0	41.0	31.0	41.0
41	36.6141832539724	39.0	35.0	41.0	31.0	41.0
42	36.51651286059866	39.0	35.0	41.0	30.0	41.0
43	36.45494511896858	39.0	35.0	41.0	30.0	41.0
44	36.29094047262139	39.0	35.0	41.0	30.0	41.0
45	36.273595566306945	39.0	35.0	41.0	30.0	41.0
46	36.16783897518185	38.0	35.0	41.0	30.0	41.0
47	36.065741928671045	38.0	35.0	40.0	30.0	41.0
48	35.910171834736666	38.0	35.0	40.0	29.0	41.0
49	35.820149464784016	38.0	35.0	40.0	29.0	41.0
50	35.74431253360652	38.0	35.0	40.0	29.0	41.0
51	34.48966406230086	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	6.0
11	3.0
12	10.0
13	16.0
14	12.0
15	29.0
16	50.0
17	78.0
18	179.0
19	335.0
20	567.0
21	886.0
22	1334.0
23	2103.0
24	3108.0
25	4486.0
26	6168.0
27	7517.0
28	8748.0
29	10074.0
30	12084.0
31	15251.0
32	19597.0
33	27140.0
34	47459.0
35	63761.0
36	60931.0
37	91170.0
38	158964.0
39	281621.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30266922207671	21.128256418161538	24.39805649657168	11.171017863190079
2	33.22332446869845	24.818873782731078	24.58133717211269	17.376464576457778
3	33.06905311862386	24.070942973006762	26.0171167158045	16.842887192564874
4	29.598858076426826	25.662450401943016	25.506965272560212	19.23172624906994
5	25.880202409837437	30.50652224311271	23.615532976563138	19.997742370486712
6	26.96313630863009	35.05467468893871	22.770499822181332	15.211689180249868
7	79.89083268901882	6.842680850082476	8.28416515652291	4.982321304375796
8	80.05772734389888	6.1661202636814165	8.682042013756973	5.09411037866273
9	72.81498483382755	8.748557119847355	11.23364887549416	7.202809170830942
10	40.226102809534964	26.062876195724343	17.683307985575446	16.027713009165247
11	30.967030112650857	24.436169166849744	24.434712631679883	20.162088088819516
12	27.25505023225667	23.06035032098394	28.83478400190321	20.849815444856183
13	24.72189281600441	23.89433808366095	30.228930915323115	21.154838185011524
14	21.01962317007597	27.048707749859506	29.36374902442488	22.567920055639643
15	20.964638967413666	25.282294722608945	32.908470116146546	20.844596193830846
16	23.70717331433364	24.636078618913352	30.62353056842499	21.033217498328018
17	23.5831250690337	25.100591960168618	27.93598042416732	23.380302546630364
18	24.003214087608164	24.818995160661895	28.767176494435432	22.410614257294508
19	25.40949879411026	25.962132513142194	27.127603404893712	21.50076528785383
20	26.746233946251426	25.507208028421857	27.168871901373148	20.57768612395357
21	25.028857603052895	25.93822106077029	27.779888405130404	21.253032931046413
22	23.681441192999404	24.73063202702358	26.71977355733226	24.86815322264475
23	23.965344173191742	25.34444022318974	27.191569574436837	23.498646029181682
24	23.23562005309071	24.386040081420315	28.559013343075932	23.819326522413043
25	23.580454754555618	25.89076228981894	26.54972307625083	23.979059879374613
26	22.451882753773944	27.38589564168264	27.211839688884087	22.95038191565933
27	24.376087091092923	26.584922676189173	26.771601933793193	22.26738829892471
28	21.96624965255567	27.466490587748353	27.403980953375097	23.16327880632088
29	22.813103475899805	26.942380682459554	26.77257295723977	23.471942884400875
30	24.692762112607163	25.736733695605995	26.62849735335422	22.942006838432622
31	24.81450417722149	27.606196586124316	24.72201419393523	22.857285042718964
32	24.886724046060497	27.979676479263187	25.96176837934973	21.171831095326585
33	25.61110753720537	26.914342380439706	25.576514826921137	21.898035255433786
34	23.65291737925627	27.278597550836114	26.86506294052603	22.203422129381593
35	25.499682596710898	25.550782705586904	26.25756639676261	22.691968300939585
36	25.763194084525164	28.19937053405076	25.202063910335692	20.835371471088386
37	25.610622025482083	27.701599639750302	26.663696953292558	20.024081381475057
38	24.49054647985794	27.050164285029364	26.510275248733723	21.94901398637897
39	25.356699394202746	24.293550098134055	27.71980632937358	22.629944178289616
40	26.01784498338943	24.39405102485456	28.288461935274007	21.299642056482007
41	23.516731340874138	25.8855430387936	27.030865194028692	23.566860426303567
42	23.85561852372878	26.20962211408797	27.961348411709086	21.97341095047416
43	24.27910612436626	24.595174256226386	28.600039083693723	22.525680535713636
44	23.652796001325445	25.601033168947158	27.003555159593773	23.742615670133624
45	24.0800463178184	24.20251665001766	27.173605640675202	24.543831391488737
46	24.568713867307217	25.305842041188388	26.866883609488358	23.258560482016037
47	23.977239210412286	24.85735058680161	28.691558043533412	22.4738521592527
48	23.207338995209213	24.177148662475894	29.079603288370905	23.535909053943993
49	23.623908053789844	24.05127974821362	29.102786473157877	23.22202572483866
50	22.390829654570545	24.35751626767718	29.285460259044783	23.966193818707495
51	23.131113654653085	23.569166606989185	27.131851632472483	26.16786810588525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	281.0
1	783.5
2	1286.0
3	3135.5
4	4985.0
5	3385.0
6	1785.0
7	1705.5
8	1626.0
9	1686.0
10	1746.0
11	1779.0
12	1812.0
13	1823.5
14	1835.0
15	1883.5
16	1932.0
17	1975.5
18	2019.0
19	2107.0
20	2195.0
21	2219.5
22	2244.0
23	2549.0
24	2854.0
25	3549.5
26	4913.5
27	5582.0
28	7719.0
29	9856.0
30	11140.0
31	12424.0
32	14337.5
33	16251.0
34	17247.5
35	18244.0
36	19810.0
37	21376.0
38	24088.0
39	26800.0
40	31083.5
41	35367.0
42	37840.5
43	40314.0
44	45268.5
45	50223.0
46	57433.5
47	64644.0
48	70687.0
49	76730.0
50	76476.0
51	76222.0
52	68498.0
53	60774.0
54	54858.0
55	48942.0
56	45842.0
57	42742.0
58	41460.0
59	40178.0
60	39715.0
61	39252.0
62	35611.0
63	31970.0
64	28482.5
65	24995.0
66	21157.5
67	17320.0
68	14721.5
69	12123.0
70	10034.0
71	7945.0
72	6420.5
73	4896.0
74	4150.0
75	2842.5
76	2281.0
77	1692.5
78	1104.0
79	846.5
80	589.0
81	431.0
82	273.0
83	181.5
84	90.0
85	72.0
86	54.0
87	43.0
88	32.0
89	23.0
90	14.0
91	13.5
92	13.0
93	8.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	823873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.868583829649275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.204756215222	22.288585701289705
2	9.66960509659293	5.969740310475656
3	3.487502187734089	3.2296276091446487
4	1.7877054194605575	2.2073573841334615
5	1.1891881422510315	1.8354276929150426
6	0.9192196851708097	1.7025005945735372
7	0.7620087876316329	1.6465492497955532
8	0.7044542792898856	1.7396404779531998
9	0.6115274635796796	1.6989288096277921
>10	8.513693032555935	51.34686448807682
>50	0.09851206105708556	2.036200371211855
>100	0.04628879882567202	2.532274576382026
>500	0.003956307591937777	0.8749078883627116
>1k	0.0015825230367751105	0.8913948460579888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2825	0.34289265457176044	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1660	0.2014873651642911	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1643	0.19942394034031946	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	1171	0.14213355699240052	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	952	0.11555179014241272	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	857	0.10402088671433583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.641337924655863E-4	0.0	0.0	0.16155402592389848	0.0
2	3.641337924655863E-4	0.0	0.0	0.7430756924914398	0.0
3	3.641337924655863E-4	0.0	0.0	1.0927655111892245	0.0
4	3.641337924655863E-4	0.0	0.0	1.4578703271013858	0.0
5	3.641337924655863E-4	0.0	0.0	2.5541557982844445	0.0
6	3.641337924655863E-4	0.0	0.0	3.417031508497062	0.0
7	3.641337924655863E-4	0.0	0.0	4.1954281788576635	0.0
8	3.641337924655863E-4	0.0	0.0	5.419281855334499	0.0
9	3.641337924655863E-4	0.0	0.0	5.943877272346588	0.0
10	3.641337924655863E-4	0.0	0.0	6.931772251305723	0.0
11	3.641337924655863E-4	0.0	0.0	8.255762720710594	0.0
12	3.641337924655863E-4	0.0	0.0	9.234918488650557	0.0
13	3.641337924655863E-4	0.0	0.0	9.661926049281867	0.0
14	3.641337924655863E-4	0.0	0.0	9.861349989622187	0.0
15	3.641337924655863E-4	0.0	0.0	10.203878510401482	0.0
16	3.641337924655863E-4	0.0	0.0	10.87376331060734	0.0
17	3.641337924655863E-4	0.0	0.0	11.64754761959671	0.0
18	3.641337924655863E-4	0.0	0.0	12.456652906455243	0.0
19	3.641337924655863E-4	0.0	0.0	12.938401913887214	0.0
20	3.641337924655863E-4	0.0	0.0	13.428890132338358	0.0
21	4.8551172328744843E-4	0.0	0.0	14.031895692661369	0.0
22	4.8551172328744843E-4	0.0	0.0	14.66621675913642	0.0
23	4.8551172328744843E-4	0.0	0.0	15.297260621479282	0.0
24	4.8551172328744843E-4	0.0	0.0	15.85450670188245	0.0
25	4.8551172328744843E-4	0.0	0.0	16.334070906559628	0.0
26	6.068896541093105E-4	0.0	0.0	16.753795791341627	0.0
27	6.068896541093105E-4	0.0	0.0	17.188450161614714	0.0
28	6.068896541093105E-4	0.0	0.0	17.627231381535747	0.0
29	6.068896541093105E-4	0.0	0.0	18.094293659338273	0.0
30	6.068896541093105E-4	0.0	0.0	18.664284422477735	0.0
31	6.068896541093105E-4	0.0	0.0	19.140814178884366	0.0
32	7.282675849311726E-4	0.0	0.0	19.635672002845098	0.0
33	7.282675849311726E-4	0.0	0.0	20.073603577250378	0.0
34	7.282675849311726E-4	0.0	0.0	20.52864943990154	0.0
35	7.282675849311726E-4	0.0	0.0	21.013918407327342	0.0
36	7.282675849311726E-4	0.0	0.0	21.509990010596294	0.0
37	7.282675849311726E-4	0.0	0.0	22.012373266267982	0.0
38	7.282675849311726E-4	0.0	0.0	22.472274246152015	0.0
39	7.282675849311726E-4	0.0	0.0	22.944677152910703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCGT	20	7.032121E-4	45.000004	45
CATTTCG	20	7.032121E-4	45.000004	30
AATACGC	20	7.032121E-4	45.000004	45
GTACGAG	35	1.2113742E-7	45.0	1
CGAATAT	180	0.0	41.25	14
TAGTGCG	50	1.0804797E-9	40.5	1
TACGAAT	185	0.0	40.135136	12
TATAGCG	45	1.9272193E-8	40.0	1
GCGATAT	40	3.4566256E-7	39.375004	9
TTGACGT	35	6.2461368E-6	38.571426	19
CGCAATC	35	6.2461368E-6	38.571426	20
GCGTAAG	35	6.2461368E-6	38.571426	1
CGTTTTT	1460	0.0	37.910957	1
GTCGATA	30	1.1396414E-4	37.499996	9
TAGCCGT	30	1.1396414E-4	37.499996	44
TTTGCGC	30	1.1396414E-4	37.499996	13
TAAGCGA	30	1.1396414E-4	37.499996	24
GCGTATG	30	1.1396414E-4	37.499996	1
GAGTACG	55	2.746674E-9	36.81818	1
CACAACG	190	0.0	36.710526	12
>>END_MODULE