FastQCFastQC Report
Sat 18 Jun 2016
SRR3550459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974046
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.40573032485118776No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC22740.23345920007884638TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC19480.1999905548608587TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT16070.1649819413046201TruSeq Adapter, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGC207.032755E-445.00000411
CCCGTAA207.032755E-445.00000418
TACGCCG207.032755E-445.0000041
TCCCGTA207.032755E-445.00000417
TCGACGT207.032755E-445.00000426
CGAATAT1400.043.39285714
GCACTAG800.042.1875041
CGTTTTT21850.040.469111
CGCATGG1550.039.193552
TACGAAT1550.039.1935512
TCGCGAG356.2473064E-638.5714261
TAGCGCG659.094947E-1238.076921
CTACGAA1600.037.96875411
ACGGGAG4700.037.819155
TAGACCG301.13979404E-437.4999961
CGGCACT301.13979404E-437.49999624
GCGCTCA301.13979404E-437.49999611
TCGCGCA301.13979404E-437.49999634
GCGAAAG1150.037.1739161
CGAAAGG1650.036.818182
Produced by FastQC (version 0.11.5)