##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974046
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2240982458734	33.0	31.0	34.0	30.0	34.0
2	32.334398991423406	34.0	31.0	34.0	30.0	34.0
3	32.42489471749794	34.0	31.0	34.0	30.0	34.0
4	35.931830734893424	37.0	35.0	37.0	35.0	37.0
5	35.91090461846771	37.0	35.0	37.0	35.0	37.0
6	35.931519661289094	37.0	35.0	37.0	35.0	37.0
7	36.14404247848664	37.0	35.0	37.0	35.0	37.0
8	36.14818704660765	37.0	36.0	37.0	35.0	37.0
9	37.71178773897742	39.0	38.0	39.0	35.0	39.0
10	37.435596470803226	39.0	37.0	39.0	35.0	39.0
11	37.365333875402186	39.0	37.0	39.0	34.0	39.0
12	37.36692414937282	39.0	37.0	39.0	35.0	39.0
13	37.35538465329153	39.0	37.0	39.0	34.0	39.0
14	38.57074511881369	40.0	38.0	41.0	34.0	41.0
15	38.63560037205635	40.0	38.0	41.0	35.0	41.0
16	38.643323826595456	40.0	38.0	41.0	35.0	41.0
17	38.65371553294197	40.0	38.0	41.0	35.0	41.0
18	38.59140841397634	40.0	38.0	41.0	35.0	41.0
19	38.542171519620226	40.0	38.0	41.0	34.0	41.0
20	38.43592705067317	40.0	38.0	41.0	34.0	41.0
21	38.39110165228336	40.0	37.0	41.0	34.0	41.0
22	38.370021539023824	40.0	37.0	41.0	34.0	41.0
23	38.33766680423717	40.0	37.0	41.0	34.0	41.0
24	38.33195968157561	40.0	37.0	41.0	34.0	41.0
25	38.30267667030099	40.0	37.0	41.0	34.0	41.0
26	38.18080973588516	40.0	37.0	41.0	34.0	41.0
27	38.12591602449987	40.0	37.0	41.0	34.0	41.0
28	38.07228098056971	40.0	37.0	41.0	34.0	41.0
29	38.03383618432805	40.0	37.0	41.0	34.0	41.0
30	38.01427858643226	40.0	37.0	41.0	34.0	41.0
31	37.9345646920166	40.0	37.0	41.0	33.0	41.0
32	37.786025505982266	40.0	37.0	41.0	33.0	41.0
33	37.696706315718146	40.0	37.0	41.0	33.0	41.0
34	37.54099395716424	40.0	36.0	41.0	33.0	41.0
35	37.5103547471064	40.0	37.0	41.0	33.0	41.0
36	37.38523642620574	40.0	36.0	41.0	32.0	41.0
37	37.378458512226324	40.0	36.0	41.0	32.0	41.0
38	37.29790687503465	40.0	36.0	41.0	32.0	41.0
39	37.225095118711025	40.0	36.0	41.0	32.0	41.0
40	37.1659849740156	40.0	36.0	41.0	32.0	41.0
41	37.05533414233003	40.0	36.0	41.0	31.0	41.0
42	36.994863692269156	40.0	35.0	41.0	31.0	41.0
43	36.93068191851309	39.0	35.0	41.0	31.0	41.0
44	36.76275453110017	39.0	35.0	41.0	31.0	41.0
45	36.72189917108638	39.0	35.0	41.0	31.0	41.0
46	36.61916788324165	39.0	35.0	41.0	31.0	41.0
47	36.5437433139708	39.0	35.0	41.0	30.0	41.0
48	36.44735669567967	39.0	35.0	41.0	30.0	41.0
49	36.371398270718224	39.0	35.0	41.0	30.0	41.0
50	36.28930255860606	39.0	35.0	41.0	30.0	41.0
51	35.06329167205656	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	7.0
13	6.0
14	9.0
15	36.0
16	45.0
17	76.0
18	155.0
19	286.0
20	509.0
21	859.0
22	1335.0
23	2055.0
24	3273.0
25	5193.0
26	7074.0
27	8536.0
28	10160.0
29	11202.0
30	13759.0
31	17082.0
32	21754.0
33	29667.0
34	48815.0
35	66097.0
36	67645.0
37	98848.0
38	187751.0
39	371497.0
40	303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44542044215571	21.736345100744728	26.13151740266887	11.686717054430694
2	32.16254673803907	24.383140015974604	25.724863096814733	17.729450149171598
3	31.34081963274835	24.396075750015914	28.04066748387653	16.222437133359204
4	27.835954359445036	26.033678080911987	27.248815764347885	18.88155179529509
5	25.03598392683713	30.3826513326886	25.494586497968267	19.086778242506
6	26.625539245579777	34.123337090856076	24.23283910616131	15.018284557402833
7	82.08544565657063	5.329214431351292	8.02929225108465	4.556047660993423
8	83.10049012058978	4.487159743995663	7.8416214429297995	4.5707286924847494
9	75.8240370577981	7.854865170638758	10.525478262833584	5.795619508729567
10	34.88100151327556	35.82418078817633	16.34830387887225	12.946513819675868
11	24.470712882143143	25.14049644472643	31.54861269385635	18.840177979274078
12	24.035312500641652	22.55150167445891	33.13354810758425	20.2796377173152
13	22.706422489286954	24.19926779638744	34.0563998004201	19.037909913905505
14	19.77925067193952	27.960999788511014	31.379421505760508	20.88032803378896
15	18.7701607521616	27.11658381637007	34.79127269143347	19.321982740034866
16	22.235089513226274	26.34013178022393	32.01419645478756	19.410582251762236
17	21.317781706408116	26.347318299135768	29.833703952380077	22.501196042076042
18	22.352640429712764	25.789131108797736	31.29277262059492	20.56545584089458
19	21.825047277027984	27.71665814550853	29.488751044611856	20.96954353285163
20	24.193005258478554	26.97470140013921	29.09513513735491	19.737158204027324
21	23.28226798323693	27.139991335111485	30.058231336097062	19.51950934555452
22	21.740554347535948	25.12099017910858	29.110021497957998	24.028433975397466
23	20.256332863129668	27.60044186824852	29.82436147779468	22.318863790827127
24	21.31398311784043	25.883479835654576	31.31628280389222	21.48625424261277
25	21.032168911940506	27.38340899711102	28.968139081727145	22.61628300922133
26	20.568227783903428	28.018594604361603	29.79068750346493	21.62249010827004
27	22.04639205951259	26.981169267159867	29.506922671003217	21.465516002324325
28	19.70615350815054	27.262059492056846	30.043447640049852	22.988339359742763
29	20.987715159242992	25.010933775201583	30.84926173917864	23.15208932637678
30	22.810011026173303	24.409422142280754	30.26725637187566	22.513310459670283
31	22.29350564552393	25.980805834632044	28.08409459101521	23.641593928828826
32	20.931762976286542	25.97454329672315	30.348053377355892	22.74564034963441
33	21.46869860355671	25.38165548649653	29.26566096467723	23.883984945269525
34	19.66447169846188	24.459009122772436	30.612003950532113	25.264515228233574
35	19.53480636438115	24.403878256263052	31.624892458877714	24.436422920478087
36	20.273580508518076	25.935428100931578	29.968913172478505	23.822078218071837
37	20.313927678980253	25.74755196366496	30.496506325163285	23.442014032191498
38	19.043145806255556	25.654332546922838	30.9156857068352	24.386835939986405
39	20.562581233329844	24.011699652788472	30.45236056613343	24.97335854774826
40	20.82324654071779	23.496118253142047	31.70918005925798	23.97145514688218
41	19.265722563410765	24.812585853234857	30.894126150099687	25.027565433254694
42	20.916157963792266	24.919254326797706	31.105512470663605	23.059075238746424
43	21.41757165472678	24.544528697823306	31.025742110742204	23.01215753670771
44	20.88576925525078	24.897797434618077	29.56503080963322	24.651402500497923
45	20.69481318130766	23.071805643675965	29.609895220554268	26.62348595446211
46	21.238319340154366	24.019912817259144	29.509489284900305	25.23227855768619
47	19.98108918880628	24.29895508015022	31.560008459559402	24.159947271484096
48	19.705126862591705	23.07858150436427	31.80609540001191	25.410196233032117
49	20.74624812380524	22.709502425963453	31.876831279015573	24.667418171215733
50	19.17527508967749	22.999529796334052	31.523254548553147	26.301940565435306
51	19.305967069317052	22.19505033643175	29.93852446393702	28.560458130314174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	614.5
2	983.0
3	3802.0
4	6621.0
5	4409.5
6	2198.0
7	2160.5
8	2123.0
9	2130.5
10	2138.0
11	2203.5
12	2269.0
13	2323.0
14	2377.0
15	2251.5
16	2126.0
17	2225.5
18	2325.0
19	2587.0
20	2849.0
21	3117.0
22	3385.0
23	3990.0
24	4595.0
25	5921.5
26	8666.0
27	10084.0
28	12076.5
29	14069.0
30	16222.0
31	18375.0
32	21403.5
33	24432.0
34	27113.5
35	29795.0
36	32173.0
37	34551.0
38	37542.0
39	40533.0
40	44590.5
41	48648.0
42	52714.5
43	56781.0
44	62421.0
45	68061.0
46	78405.5
47	88750.0
48	96745.0
49	104740.0
50	101931.5
51	99123.0
52	86122.5
53	73122.0
54	63624.5
55	54127.0
56	47719.0
57	41311.0
58	37228.0
59	33145.0
60	31115.0
61	29085.0
62	26501.0
63	23917.0
64	19558.5
65	15200.0
66	12223.0
67	9246.0
68	7500.5
69	5755.0
70	4793.0
71	3831.0
72	3007.5
73	2184.0
74	1897.0
75	1322.5
76	1035.0
77	847.5
78	660.0
79	414.5
80	169.0
81	137.5
82	106.0
83	82.0
84	58.0
85	38.5
86	19.0
87	20.5
88	22.0
89	17.0
90	12.0
91	8.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	974046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.39119224232644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.58942474225258	23.18866819343864
2	10.543000072349427	6.830006843086636
3	3.9338196679671693	3.82263327335308
4	1.998835236880834	2.5897862567417276
5	1.26572016117358	2.0499092532780914
6	0.9458332393378024	1.8381999770743866
7	0.7535418024203393	1.7085682169378464
8	0.6477452195661495	1.6784991944812078
9	0.5865588603637772	1.7099406726734718
>10	7.600117192790131	48.94389162287833
>50	0.0958046655733035	2.0283716847260513
>100	0.03576696455157295	2.002506410689515
>500	0.0025547831822192364	0.5972659752684983
>1k	0.0012773915911096182	1.0117524253725636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3952	0.40573032485118776	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	2274	0.23345920007884638	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1948	0.1999905548608587	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1607	0.1649819413046201	TruSeq Adapter, Index 16 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1832562322518649	0.0
2	0.0	0.0	0.0	0.8336361937731893	0.0
3	0.0	0.0	0.0	1.2162669935506125	0.0
4	0.0	0.0	0.0	1.6657324192081278	0.0
5	0.0	0.0	0.0	2.943290152621129	0.0
6	0.0	0.0	0.0	3.929383211881164	0.0
7	0.0	0.0	0.0	4.869995872884854	0.0
8	0.0	0.0	0.0	6.471665609221741	0.0
9	0.0	0.0	0.0	7.19770934842913	0.0
10	0.0	0.0	0.0	8.43779451894469	0.0
11	0.0	0.0	0.0	9.987721319116346	0.0
12	0.0	0.0	0.0	11.234993008543745	0.0
13	0.0	0.0	0.0	11.7763432117169	0.0
14	0.0	0.0	0.0	12.002821221995676	0.0
15	0.0	0.0	0.0	12.376930863634778	0.0
16	0.0	0.0	0.0	13.066939343727093	0.0
17	0.0	0.0	0.0	14.005909371836648	0.0
18	0.0	0.0	0.0	14.980504000837742	0.0
19	0.0	0.0	0.0	15.609940392958855	0.0
20	0.0	0.0	0.0	16.20231488040606	0.0
21	0.0	0.0	0.0	16.92456003104576	0.0
22	0.0	0.0	0.0	17.741667231321724	0.0
23	0.0	0.0	0.0	18.5246897990444	0.0
24	0.0	0.0	0.0	19.14930095703899	0.0
25	0.0	0.0	0.0	19.68572326152974	0.0
26	0.0	0.0	0.0	20.19196218659078	0.0
27	0.0	0.0	0.0	20.705900953343065	0.0
28	0.0	0.0	0.0	21.234623416142565	0.0
29	0.0	0.0	0.0	21.81765542900438	0.0
30	0.0	0.0	0.0	22.522447605143906	0.0
31	0.0	0.0	0.0	23.109278206573407	0.0
32	0.0	0.0	0.0	23.701242035797076	0.0
33	0.0	0.0	0.0	24.245364181979085	0.0
34	0.0	0.0	0.0	24.773573321999166	0.0
35	0.0	0.0	0.0	25.34808417672266	0.0
36	1.0266455588339771E-4	0.0	0.0	25.899700835484154	0.0
37	1.0266455588339771E-4	0.0	0.0	26.4479295639015	0.0
38	1.0266455588339771E-4	0.0	0.0	26.99420766575706	0.0
39	1.0266455588339771E-4	0.0	0.0	27.556090780106896	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTCGC	20	7.032755E-4	45.000004	11
CCCGTAA	20	7.032755E-4	45.000004	18
TACGCCG	20	7.032755E-4	45.000004	1
TCCCGTA	20	7.032755E-4	45.000004	17
TCGACGT	20	7.032755E-4	45.000004	26
CGAATAT	140	0.0	43.392857	14
GCACTAG	80	0.0	42.187504	1
CGTTTTT	2185	0.0	40.46911	1
CGCATGG	155	0.0	39.19355	2
TACGAAT	155	0.0	39.19355	12
TCGCGAG	35	6.2473064E-6	38.571426	1
TAGCGCG	65	9.094947E-12	38.07692	1
CTACGAA	160	0.0	37.968754	11
ACGGGAG	470	0.0	37.81915	5
TAGACCG	30	1.13979404E-4	37.499996	1
CGGCACT	30	1.13979404E-4	37.499996	24
GCGCTCA	30	1.13979404E-4	37.499996	11
TCGCGCA	30	1.13979404E-4	37.499996	34
GCGAAAG	115	0.0	37.173916	1
CGAAAGG	165	0.0	36.81818	2
>>END_MODULE