##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330300
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.294777475022705	33.0	31.0	34.0	30.0	34.0
2	32.38833484710869	34.0	31.0	34.0	30.0	34.0
3	32.50020890099909	34.0	31.0	34.0	31.0	34.0
4	36.001108083560396	37.0	35.0	37.0	35.0	37.0
5	35.964362700575236	37.0	35.0	37.0	35.0	37.0
6	35.97037238873751	37.0	35.0	37.0	35.0	37.0
7	36.13338782924614	37.0	35.0	37.0	35.0	37.0
8	36.104617014835	37.0	35.0	37.0	35.0	37.0
9	37.68200726612171	39.0	38.0	39.0	35.0	39.0
10	37.45092945806842	39.0	37.0	39.0	35.0	39.0
11	37.38966091432032	39.0	37.0	39.0	35.0	39.0
12	37.20821677263094	39.0	37.0	39.0	34.0	39.0
13	37.06287011807448	39.0	37.0	39.0	33.0	39.0
14	38.2485498032092	40.0	37.0	41.0	33.0	41.0
15	38.33232515894641	40.0	37.0	41.0	34.0	41.0
16	38.36895549500454	40.0	37.0	41.0	34.0	41.0
17	38.35507720254314	40.0	37.0	41.0	34.0	41.0
18	38.34003330305783	40.0	37.0	41.0	34.0	41.0
19	38.30471389645776	40.0	37.0	41.0	34.0	41.0
20	38.216575840145325	40.0	37.0	41.0	34.0	41.0
21	38.14985467756585	40.0	36.0	41.0	34.0	41.0
22	38.127326672721765	40.0	36.0	41.0	34.0	41.0
23	38.09725703905541	40.0	36.0	41.0	34.0	41.0
24	38.07525280048441	40.0	36.0	41.0	34.0	41.0
25	38.021574326369965	40.0	36.0	41.0	34.0	41.0
26	37.88353012412958	40.0	36.0	41.0	34.0	41.0
27	37.81751740841659	40.0	36.0	41.0	33.0	41.0
28	37.73268240993037	40.0	36.0	41.0	33.0	41.0
29	37.64561307901907	40.0	36.0	41.0	33.0	41.0
30	37.623693611868	40.0	36.0	41.0	33.0	41.0
31	37.52193763245534	40.0	35.0	41.0	33.0	41.0
32	37.34739025128671	39.0	35.0	41.0	33.0	41.0
33	37.25252800484408	39.0	35.0	41.0	33.0	41.0
34	37.04607326672722	39.0	35.0	41.0	32.0	41.0
35	37.01468362095065	39.0	35.0	41.0	32.0	41.0
36	36.91554344535271	39.0	35.0	41.0	31.0	41.0
37	36.847387223736	39.0	35.0	41.0	31.0	41.0
38	36.7577838328792	39.0	35.0	41.0	31.0	41.0
39	36.65094156827127	39.0	35.0	41.0	31.0	41.0
40	36.5286285195277	39.0	35.0	41.0	31.0	41.0
41	36.43605812897366	39.0	35.0	41.0	31.0	41.0
42	36.381577353920676	39.0	35.0	41.0	30.0	41.0
43	36.269397517408414	38.0	35.0	41.0	30.0	41.0
44	36.06518013926733	38.0	35.0	40.0	30.0	41.0
45	35.963802603693615	38.0	35.0	40.0	30.0	41.0
46	35.860115046927035	38.0	35.0	40.0	30.0	41.0
47	35.74515894641235	38.0	35.0	40.0	29.0	41.0
48	35.65267029972752	38.0	35.0	40.0	29.0	41.0
49	35.587411444141686	37.0	35.0	40.0	29.0	41.0
50	35.480974871329096	37.0	34.0	40.0	28.0	41.0
51	34.22719648804117	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	2.0
14	8.0
15	12.0
16	18.0
17	41.0
18	83.0
19	138.0
20	252.0
21	360.0
22	583.0
23	905.0
24	1280.0
25	1907.0
26	2450.0
27	3049.0
28	3554.0
29	4212.0
30	5047.0
31	6506.0
32	8462.0
33	11393.0
34	21711.0
35	30175.0
36	24434.0
37	36802.0
38	63891.0
39	102950.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95610051468362	20.759006963366637	23.92310021192855	10.361792310021192
2	32.46260974871329	24.033605812897367	24.79533757190433	18.708446866485012
3	32.13139570087799	24.981531940660005	25.339388434756284	17.547683923705723
4	29.453527096578867	26.12897366030881	24.456251892219193	19.961247350893128
5	25.716318498334843	30.58098698153194	23.520435967302454	20.18225855283076
6	27.246745382985164	34.6924008477142	22.845594913714802	15.21525885558583
7	81.58764759309717	6.083863154707841	7.621858916136845	4.706630336058129
8	81.64971238268241	5.1692400847714195	8.097184377838328	5.083863154707841
9	75.26158038147139	7.653648198607327	10.204359673024523	6.88041174689676
10	41.05449591280654	26.891008174386922	16.854677565849226	15.199818346957311
11	33.16197396306388	24.867393278837422	22.747199515591888	19.22343324250681
12	30.25673630033303	21.913109294580686	27.01967907962458	20.810475325461702
13	25.40993036633364	25.72146533454435	28.038147138964575	20.830457160157433
14	20.966091432031487	28.003027550711472	28.011201937632457	23.019679079624584
15	20.412352406902816	25.37026945201332	33.20799273387829	21.00938540720557
16	24.4729034211323	24.27338782924614	30.68876778686043	20.564940962761128
17	23.311232213139572	23.592188919164396	27.214653345443534	25.881925522252498
18	25.38570996064184	23.844686648501362	28.070541931577353	22.69906145927944
19	25.468362095065096	26.683923705722073	25.09688162276718	22.750832576445656
20	29.653648198607325	24.32334241598547	25.308204662428096	20.71480472297911
21	25.591886164093246	27.021495610051467	25.419618528610354	21.96699969724493
22	24.245231607629428	24.59551922494702	25.5501059642749	25.609143203148655
23	24.408719346049047	26.903723887375115	25.86739327883742	22.82016348773842
24	24.98304571601574	23.505903723887375	28.112927641537993	23.398122918558887
25	22.850438994853164	25.673024523160766	26.7511353315168	24.72540115046927
26	22.921586436572813	27.69936421435059	26.601877081441117	22.777172267635482
27	23.878897971541026	27.184377838328793	25.36330608537693	23.573418104753255
28	21.744777475022705	28.544050862851954	26.986981531940664	22.72419013018468
29	22.952467453829854	26.78201634877384	27.25643354526188	23.009082652134424
30	24.019073569482288	26.012110202845896	27.180442022403877	22.78837420526794
31	25.08265213442325	27.715712988192553	24.185588858613382	23.016046018770815
32	24.997275204359674	28.170148349984864	25.077202543142597	21.75537390251287
33	25.131395700877988	27.48138056312443	24.053587647593098	23.33363608840448
34	22.407811080835604	27.455343627005753	27.00151377535574	23.135331516802907
35	21.46745382985165	27.89131092945807	26.127762640024223	24.513472600666063
36	24.021495610051467	30.87072358462004	22.92310021192855	22.18468059339994
37	24.663639115955192	28.169240084771417	25.24190130184681	21.925219497426582
38	23.41659097789888	28.089009990917347	24.350893127459887	24.143505903723888
39	25.141992128368152	25.170451105056006	25.153799576142898	24.53375719043294
40	24.300635785649412	24.711777172267634	26.98153194066001	24.006055101422948
41	20.91462306993642	26.657584014532244	25.093248561913413	27.334544353617922
42	25.168634574629124	25.55858310626703	24.929155313351497	24.343627005752346
43	24.91946715107478	25.125946109597336	25.736905843172874	24.21768089615501
44	23.927036027853465	26.057523463518013	25.108689070541935	24.906751438086587
45	23.09355131698456	25.079927338782927	26.004844081138355	25.821677263094156
46	23.003027550711476	26.76112624886467	25.077505298213744	25.158340902210114
47	21.760520738722374	26.605207387223732	27.535270965788676	24.099000908265214
48	22.646987587042084	24.214653345443537	28.119588253103238	25.01877081441114
49	23.58462004238571	23.97396306388132	27.823493793521042	24.61792310021193
50	20.72661217075386	25.44595821980018	27.53648198607327	26.29094762337269
51	20.44747199515592	25.188919164396	25.788676960339085	28.574931880108988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	113.0
1	299.0
2	485.0
3	794.5
4	1104.0
5	800.0
6	496.0
7	546.0
8	596.0
9	584.0
10	572.0
11	587.0
12	602.0
13	643.5
14	685.0
15	705.0
16	725.0
17	692.0
18	659.0
19	638.0
20	617.0
21	741.0
22	865.0
23	912.0
24	959.0
25	1281.5
26	1833.5
27	2063.0
28	2302.5
29	2542.0
30	3013.5
31	3485.0
32	4397.5
33	5310.0
34	6628.0
35	7946.0
36	8011.0
37	8076.0
38	9091.0
39	10106.0
40	11354.5
41	12603.0
42	14553.0
43	16503.0
44	18556.5
45	20610.0
46	23392.5
47	26175.0
48	28539.0
49	30903.0
50	30957.0
51	31011.0
52	27784.5
53	24558.0
54	23008.0
55	21458.0
56	19682.0
57	17906.0
58	17651.0
59	17396.0
60	16948.0
61	16500.0
62	15554.0
63	14608.0
64	12379.5
65	10151.0
66	8511.5
67	6872.0
68	5758.0
69	4644.0
70	3866.0
71	3088.0
72	2610.5
73	2133.0
74	1834.0
75	1315.0
76	1095.0
77	803.5
78	512.0
79	368.0
80	224.0
81	149.0
82	74.0
83	82.5
84	91.0
85	55.0
86	19.0
87	13.5
88	8.0
89	7.0
90	6.0
91	5.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	330300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.060663283197353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.32419868442467	22.15376919280782
2	9.521657820845604	5.91498014942216
3	3.347980454880363	3.119714807632946
4	1.9003696018504108	2.3610696126679773
5	1.2518213877019473	1.9441201307057516
6	1.032019874270141	1.9233133089763508
7	0.9421045107980758	2.048367369123628
8	0.862180412259348	2.142391637964471
9	0.7932459351610582	2.2174870403522364
>10	8.89954003089176	52.296822082001185
>50	0.0979069289426493	1.9763748877884502
>100	0.023977207087995743	1.3033089227055252
>500	0.002997150885999468	0.5982808578514888
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	899	0.2721768089615501	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	518	0.15682712685437483	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	511	0.15470784135634272	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	476	0.14411141386618226	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	468	0.14168937329700274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.082652134423251E-4	0.0	0.0	0.16621253405994552	0.0
2	9.082652134423251E-4	0.0	0.0	0.7971541023312141	0.0
3	9.082652134423251E-4	0.0	0.0	1.1144414168937329	0.0
4	9.082652134423251E-4	0.0	0.0	1.4865273993339387	0.0
5	9.082652134423251E-4	0.0	0.0	2.616106569785044	0.0
6	9.082652134423251E-4	0.0	0.0	3.3094156827126855	0.0
7	9.082652134423251E-4	0.0	0.0	3.9579170451105057	0.0
8	9.082652134423251E-4	0.0	0.0	5.10414774447472	0.0
9	9.082652134423251E-4	0.0	0.0	5.577656675749319	0.0
10	9.082652134423251E-4	0.0	0.0	6.557069330911292	0.0
11	9.082652134423251E-4	0.0	0.0	7.782621858916137	0.0
12	9.082652134423251E-4	0.0	0.0	8.729336966394188	0.0
13	9.082652134423251E-4	0.0	0.0	9.129276415379957	0.0
14	9.082652134423251E-4	0.0	0.0	9.284589766878595	0.0
15	9.082652134423251E-4	0.0	0.0	9.614592794429306	0.0
16	9.082652134423251E-4	0.0	0.0	10.265818952467454	0.0
17	9.082652134423251E-4	0.0	0.0	11.054193157735392	0.0
18	9.082652134423251E-4	0.0	0.0	11.892824704813806	0.0
19	9.082652134423251E-4	0.0	0.0	12.369361186799878	0.0
20	9.082652134423251E-4	0.0	0.0	12.837117771722676	0.0
21	0.0012110202845897668	0.0	0.0	13.410233121404783	0.0
22	0.0012110202845897668	0.0	0.0	14.000908265213443	0.0
23	0.0012110202845897668	0.0	0.0	14.637602179836513	0.0
24	0.0012110202845897668	0.0	0.0	15.135937026945202	0.0
25	0.0012110202845897668	0.0	0.0	15.556766575840145	0.0
26	0.0012110202845897668	0.0	0.0	15.950045413260671	0.0
27	0.0012110202845897668	0.0	0.0	16.341507720254313	0.0
28	0.0012110202845897668	0.0	0.0	16.727217680896153	0.0
29	0.0012110202845897668	0.0	0.0	17.136845292158643	0.0
30	0.0012110202845897668	0.0	0.0	17.63427187405389	0.0
31	0.0012110202845897668	0.0	0.0	18.06993642143506	0.0
32	0.0012110202845897668	0.0	0.0	18.49379352104148	0.0
33	0.0012110202845897668	0.0	0.0	18.91250378443839	0.0
34	0.0012110202845897668	0.0	0.0	19.320012110202846	0.0
35	0.0015137753557372085	0.0	0.0	19.747199515591888	0.0
36	0.0015137753557372085	0.0	0.0	20.192249470178627	0.0
37	0.0015137753557372085	0.0	0.0	20.626702997275203	0.0
38	0.0015137753557372085	0.0	0.0	21.05691795337572	0.0
39	0.0015137753557372085	0.0	0.0	21.522252497729337	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCACG	25	3.8847495E-5	45.000004	44
ACGTTAG	20	7.02598E-4	45.0	1
CCGATAT	20	7.02598E-4	45.0	11
TTGAGCG	45	3.8380676E-10	45.0	1
TGCGCGA	20	7.02598E-4	45.0	21
TCGTGAG	20	7.02598E-4	45.0	1
GTAGACG	20	7.02598E-4	45.0	1
CTCATAC	20	7.02598E-4	45.0	33
ATGACGT	20	7.02598E-4	45.0	44
ACGAACC	20	7.02598E-4	45.0	16
ATAGCGG	30	2.1607884E-6	44.999996	2
CGAATAT	115	0.0	43.043476	14
CCTATGC	110	0.0	42.954548	35
CAGGGTA	80	0.0	42.1875	5
GCTACGA	120	0.0	41.249996	10
TGACTAT	115	0.0	41.086956	23
TGATTCG	55	6.002665E-11	40.909092	15
ATTCGGC	50	1.0768417E-9	40.500004	17
CGAACGG	45	1.9212166E-8	40.0	2
TAGACGG	45	1.9212166E-8	40.0	2
>>END_MODULE