FastQCFastQC Report
Sat 18 Jun 2016
SRR3550457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789244
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150401.9056210753581908No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT23190.29382548362737No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG19780.2506195802565493No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC15120.1915757357673926No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT14110.17877867934377709No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC9610.12176209131776738No Hit
CGTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT9270.11745417133357998No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC8150.1032633760915509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAG207.0319424E-445.01
CGTTTTT79650.043.135591
TACGCGG700.041.7857172
CGAATAT556.002665E-1140.90909214
TATAGCG403.456389E-739.3751
ACACGAC5050.039.20792426
AACACGT4500.039.041
CGTGCGG1100.038.8636362
GACACGA5100.038.8235325
CACGACC5050.038.7623827
GCGTAAG356.2458003E-638.571431
GCTACGA659.094947E-1238.07692710
GCGAGAC5250.037.71428721
GTTTTTT93750.037.6562
TTCGTAG301.13959795E-437.4999961
ATAGTCG301.13959795E-437.4999961
TGGTCAA5250.037.28571714
TCAAGCG5350.037.00934617
AGACACG5300.036.93396424
CGAGACA5400.036.66666422