##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550457_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 789244 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23706737080041 33.0 31.0 34.0 30.0 34.0 2 32.40109522530422 34.0 31.0 34.0 30.0 34.0 3 32.377918615789284 34.0 31.0 34.0 30.0 34.0 4 35.946846602571576 37.0 35.0 37.0 35.0 37.0 5 35.930479040702245 37.0 35.0 37.0 35.0 37.0 6 35.94467870519129 37.0 35.0 37.0 35.0 37.0 7 36.10194185828464 37.0 35.0 37.0 35.0 37.0 8 36.10258677924698 37.0 35.0 37.0 35.0 37.0 9 37.6921154928007 39.0 38.0 39.0 35.0 39.0 10 37.47622154872258 39.0 37.0 39.0 35.0 39.0 11 37.42692500671529 39.0 37.0 39.0 35.0 39.0 12 37.297112173168244 39.0 37.0 39.0 34.0 39.0 13 37.22735934641251 39.0 37.0 39.0 34.0 39.0 14 38.36186401163645 40.0 38.0 41.0 34.0 41.0 15 38.425024453781084 40.0 38.0 41.0 34.0 41.0 16 38.46192432251623 40.0 38.0 41.0 34.0 41.0 17 38.49020581721242 40.0 38.0 41.0 34.0 41.0 18 38.30636279781665 40.0 38.0 41.0 34.0 41.0 19 38.11737688218092 40.0 37.0 41.0 34.0 41.0 20 37.862497529281185 40.0 35.0 41.0 34.0 41.0 21 37.78730405299248 40.0 35.0 41.0 33.0 41.0 22 37.771592815403096 40.0 35.0 41.0 33.0 41.0 23 37.74296161896701 39.0 35.0 41.0 33.0 41.0 24 37.7123373760206 39.0 35.0 41.0 33.0 41.0 25 37.64993462097906 39.0 35.0 41.0 33.0 41.0 26 37.53090805885125 39.0 35.0 41.0 33.0 41.0 27 37.48051553131858 39.0 35.0 41.0 33.0 41.0 28 37.36727551935777 39.0 35.0 41.0 33.0 41.0 29 37.275914672775464 39.0 35.0 41.0 33.0 41.0 30 37.17178971268708 39.0 35.0 41.0 33.0 41.0 31 36.93938629878719 39.0 35.0 41.0 32.0 41.0 32 36.57433315932715 39.0 35.0 41.0 31.0 41.0 33 36.1858968836 39.0 35.0 41.0 30.0 41.0 34 35.750435860139575 39.0 35.0 41.0 27.0 41.0 35 35.56015376740273 39.0 35.0 41.0 25.0 41.0 36 35.349308958953124 39.0 35.0 41.0 24.0 41.0 37 35.259145460719374 39.0 35.0 41.0 23.0 41.0 38 35.103708105478155 38.0 35.0 41.0 23.0 41.0 39 34.96990791187516 38.0 34.0 41.0 23.0 41.0 40 34.871620183365344 38.0 34.0 41.0 22.0 41.0 41 34.70627081105463 38.0 34.0 40.0 21.0 41.0 42 34.62089163807391 38.0 34.0 40.0 21.0 41.0 43 34.54841848655169 38.0 34.0 40.0 21.0 41.0 44 34.33537663891015 38.0 34.0 40.0 20.0 41.0 45 34.246914769070145 38.0 34.0 40.0 20.0 41.0 46 34.1265337462179 37.0 34.0 40.0 20.0 41.0 47 34.028604588695 37.0 33.0 40.0 20.0 41.0 48 33.85801222435647 37.0 33.0 40.0 20.0 41.0 49 33.74389288990477 36.0 33.0 40.0 20.0 41.0 50 33.620451976828456 36.0 33.0 40.0 20.0 41.0 51 32.42661204899879 35.0 31.0 39.0 16.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 8.0 11 5.0 12 6.0 13 13.0 14 15.0 15 37.0 16 67.0 17 138.0 18 264.0 19 509.0 20 895.0 21 1537.0 22 2237.0 23 3575.0 24 5696.0 25 9911.0 26 15034.0 27 17589.0 28 17363.0 29 16258.0 30 16924.0 31 18481.0 32 21966.0 33 28545.0 34 47726.0 35 57792.0 36 59136.0 37 81983.0 38 140270.0 39 225103.0 40 156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.509059302319685 20.540796002250257 22.249012979509505 19.701131715920553 2 40.77965749502055 22.220251278438607 20.440193400266583 16.55989782627426 3 28.98723335242333 22.385599383714037 32.276203556821464 16.35096370704117 4 26.779931174643075 23.614496910967965 31.832867909037006 17.772704005351958 5 24.274368889722318 26.93843728935538 30.589779586541045 18.197414234381256 6 25.192209253412123 30.677331725043206 29.760631693113915 14.369827328430752 7 73.31649021088535 5.528328375001901 16.3879611374936 4.7672202766191445 8 74.32251623072206 4.680428359290663 16.40760018448034 4.589455225506941 9 67.46177354531679 6.730238050590185 19.062165819442402 6.745822584650628 10 36.38722625702571 23.920485933374216 25.011783428192043 14.68050438140803 11 28.845071992945147 22.704258759014955 30.934539888804984 17.516129359234913 12 25.029015108128792 21.55974578203952 34.037255905651485 19.373983204180202 13 23.09121133641814 21.658701238146886 36.87098033054417 18.379107094890806 14 20.293344010217375 23.248070305254142 37.045957904019545 19.41262778050894 15 20.29511785962263 22.740622671822656 37.86864391747039 19.09561555108433 16 23.407843455255918 23.76350026100927 35.24689449650552 17.581761787229297 17 23.306480632098562 23.351713791932532 33.71770960564794 19.624095970320965 18 24.108894080917942 22.287657555838244 34.155597001687696 19.447851361556122 19 22.79244441516185 25.03687072692349 32.23223743227696 19.938447425637698 20 23.767301366877668 25.244537810867108 31.959824845041584 19.028335977213636 21 22.827541292680085 25.594239550759966 33.361926096365636 18.216293060194314 22 22.545625940773704 22.80220058689075 33.50041305350437 21.151760418831188 23 20.792429210738376 25.180299121691135 33.53082190045157 20.496449767118914 24 21.081693367323666 23.94240564388199 34.334755791618306 20.64114519717603 25 22.552974745452612 24.56350634277866 32.28811368854245 20.595405223226276 26 21.635514492349643 24.395370759866402 32.21411882763759 21.754995920146367 27 22.468083381058328 23.012275037884354 33.02590833759902 21.493733243458298 28 21.076498522636854 24.782196633740643 32.179275357177254 21.962029486445257 29 22.814870939785415 24.43148126561621 31.195675861964105 21.557971932634267 30 24.18580312298858 23.2037240701228 31.647500646188 20.96297216070062 31 24.060620036389256 23.89197763936121 30.45458692115493 21.592815403094605 32 24.446939096147705 24.001069377784308 30.637546816953947 20.91444470911404 33 23.150381884436243 25.549639908570732 28.948208665507753 22.351769541485268 34 21.549229389136944 26.817055308624454 31.097227219972527 20.536488082266064 35 21.75727658366741 27.41560277936861 29.845649761037148 20.981470875926835 36 22.377110247274608 29.09531146261486 27.051203430117937 21.4763748599926 37 22.074542220149915 28.533761422323135 28.27173852446138 21.119957833065566 38 21.618029405355 28.309496176087496 28.711653176964287 21.36082124159322 39 21.78097014358044 26.904987557713454 28.8009791648717 22.513063133834404 40 23.469041259737168 25.28508294013005 30.162535286932812 21.083340513199975 41 20.796737130722565 25.891359326139952 29.50038264465742 23.811520898480065 42 21.954934088824242 26.160224214564824 29.735037580266688 22.14980411634425 43 22.52079204910015 24.743805464469794 30.351957062708113 22.38344542372194 44 23.256052627577784 25.172696909954333 28.996736117094336 22.574514345373547 45 22.566151912463067 24.3323991059799 28.96911474778395 24.13233423377308 46 21.91476907014814 24.93310053671615 28.98127828656284 24.17085210657287 47 21.773114524785743 25.493890355834193 30.532509591457142 22.200485527922922 48 21.348404295756445 25.380744104484798 30.43646831651555 22.834383283243202 49 22.633938300449543 24.735443031559313 29.81600113526362 22.81461753272752 50 20.146494620168163 24.809057781877343 31.421968364662895 23.622479233291603 51 20.95575005955066 23.575092113465544 30.373243255571154 25.095914571412642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 317.0 1 727.5 2 1138.0 3 12453.5 4 23769.0 5 15480.0 6 7191.0 7 6980.5 8 6770.0 9 6675.0 10 6580.0 11 6601.0 12 6622.0 13 6449.0 14 6276.0 15 5918.0 16 5560.0 17 5279.5 18 4999.0 19 4690.5 20 4382.0 21 4309.0 22 4236.0 23 4300.0 24 4364.0 25 4899.5 26 5921.0 27 6407.0 28 7358.5 29 8310.0 30 9309.0 31 10308.0 32 11663.0 33 13018.0 34 15092.5 35 17167.0 36 18567.5 37 19968.0 38 21505.5 39 23043.0 40 26107.5 41 29172.0 42 31580.0 43 33988.0 44 37567.0 45 41146.0 46 49599.0 47 58052.0 48 63095.0 49 68138.0 50 70340.0 51 72542.0 52 65997.5 53 59453.0 54 54693.5 55 49934.0 56 45793.5 57 41653.0 58 38239.5 59 34826.0 60 32087.0 61 29348.0 62 26718.5 63 24089.0 64 21604.5 65 19120.0 66 16317.0 67 13514.0 68 11607.5 69 9701.0 70 8129.5 71 6558.0 72 5631.0 73 4704.0 74 3842.5 75 2709.5 76 2438.0 77 1733.0 78 1028.0 79 784.5 80 541.0 81 386.0 82 231.0 83 174.5 84 118.0 85 90.0 86 62.0 87 42.5 88 23.0 89 16.0 90 9.0 91 8.0 92 7.0 93 6.0 94 5.0 95 4.0 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 789244.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.334053648506476 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37840281036155 28.46935979135974 2 8.274836775525953 6.509657473223444 3 3.926494482448236 4.633348338695507 4 2.5928599269946693 4.079507658858836 5 2.0515722549099236 4.034832656920717 6 1.7062560966951017 4.026838130729783 7 1.4644541890290879 4.032204374593514 8 1.2390231395812146 3.8988642115222745 9 1.092240470911108 3.8666020751898746 >10 5.196373471084001 29.75608429383596 >50 0.04907473357622509 1.3192129059469007 >100 0.024860192772489756 1.9764042996506452 >500 0.001937157878369354 0.5702021014853302 >1k 0.0012914385855795692 0.9168951386913328 >5k 0.0 0.0 >10k+ 3.228596463948923E-4 1.9099865492960728 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15040 1.9056210753581908 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT 2319 0.29382548362737 No Hit CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG 1978 0.2506195802565493 No Hit CGTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC 1512 0.1915757357673926 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT 1411 0.17877867934377709 No Hit CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC 961 0.12176209131776738 No Hit CGTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT 927 0.11745417133357998 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC 815 0.1032633760915509 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2670352894668822E-4 0.0 0.0 0.06411198564702424 0.0 2 1.2670352894668822E-4 0.0 0.0 0.3356376481797771 0.0 3 1.2670352894668822E-4 0.0 0.0 0.7879692465194541 0.0 4 1.2670352894668822E-4 0.0 0.0 1.1483140828438354 0.0 5 1.2670352894668822E-4 0.0 0.0 2.1549736203252734 0.0 6 1.2670352894668822E-4 0.0 0.0 3.5534004693098713 0.0 7 1.2670352894668822E-4 0.0 0.0 4.627339580662 0.0 8 1.2670352894668822E-4 0.0 0.0 6.053007688370137 0.0 9 1.2670352894668822E-4 0.0 0.0 6.980350816730947 0.0 10 1.2670352894668822E-4 0.0 0.0 8.165282219440376 0.0 11 1.2670352894668822E-4 0.0 0.0 9.331081389278854 0.0 12 1.2670352894668822E-4 0.0 0.0 10.117150082864107 0.0 13 1.2670352894668822E-4 0.0 0.0 10.554910775374916 0.0 14 1.2670352894668822E-4 0.0 0.0 10.847089113125978 0.0 15 2.5340705789337643E-4 0.0 0.0 11.128370947387626 0.0 16 2.5340705789337643E-4 0.0 0.0 11.534582461190709 0.0 17 2.5340705789337643E-4 0.0 0.0 11.96955567606469 0.0 18 2.5340705789337643E-4 0.0 0.0 12.445834241375291 0.0 19 2.5340705789337643E-4 0.0 0.0 12.766647576668305 0.0 20 2.5340705789337643E-4 0.0 0.0 13.080365514340306 0.0 21 2.5340705789337643E-4 0.0 0.0 13.471499308198732 0.0 22 2.5340705789337643E-4 0.0 0.0 13.868208057330813 0.0 23 2.5340705789337643E-4 0.0 0.0 14.258328222957665 0.0 24 2.5340705789337643E-4 0.0 0.0 14.604608967568964 0.0 25 2.5340705789337643E-4 0.0 0.0 14.922888232283045 0.0 26 2.5340705789337643E-4 0.0 0.0 15.230651104094552 0.0 27 2.5340705789337643E-4 0.0 0.0 15.568316008737476 0.0 28 2.5340705789337643E-4 0.0 0.0 15.875572066433195 0.0 29 2.5340705789337643E-4 0.0 0.0 16.215264227539265 0.0 30 2.5340705789337643E-4 0.0 0.0 16.652518105934288 0.0 31 3.801105868400647E-4 0.0 0.0 17.002599956413984 0.0 32 3.801105868400647E-4 0.0 0.0 17.369913486830434 0.0 33 3.801105868400647E-4 0.0 0.0 17.699596069149717 0.0 34 3.801105868400647E-4 0.0 0.0 18.05195858315046 0.0 35 3.801105868400647E-4 0.0 0.0 18.42446695825372 0.0 36 3.801105868400647E-4 0.0 0.0 18.79102026749649 0.0 37 3.801105868400647E-4 0.0 0.0 19.172904703741807 0.0 38 7.602211736801294E-4 0.0 0.0 19.60280977745792 0.0 39 8.869247026268176E-4 0.0 0.0 20.19692262468894 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGACAG 20 7.0319424E-4 45.0 1 CGTTTTT 7965 0.0 43.13559 1 TACGCGG 70 0.0 41.785717 2 CGAATAT 55 6.002665E-11 40.909092 14 TATAGCG 40 3.456389E-7 39.375 1 ACACGAC 505 0.0 39.207924 26 AACACGT 450 0.0 39.0 41 CGTGCGG 110 0.0 38.863636 2 GACACGA 510 0.0 38.82353 25 CACGACC 505 0.0 38.76238 27 GCGTAAG 35 6.2458003E-6 38.57143 1 GCTACGA 65 9.094947E-12 38.076927 10 GCGAGAC 525 0.0 37.714287 21 GTTTTTT 9375 0.0 37.656 2 TTCGTAG 30 1.13959795E-4 37.499996 1 ATAGTCG 30 1.13959795E-4 37.499996 1 TGGTCAA 525 0.0 37.285717 14 TCAAGCG 535 0.0 37.009346 17 AGACACG 530 0.0 36.933964 24 CGAGACA 540 0.0 36.666664 22 >>END_MODULE