SRR3550455_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718022
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2622	0.36516986944689717	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1426	0.19860115706761072	Illumina PCR Primer Index 5 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1275	0.17757116077223262	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1114	0.15514844949040557	RNA PCR Primer, Index 33 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	988	0.13760024066114965	Illumina PCR Primer Index 5 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.031516E-4	45.0	1
CGACGGT	80	0.0	45.0	28
GCAAACG	20	7.031516E-4	45.0	1
TCGATTG	20	7.031516E-4	45.0	1
TATTGCG	25	3.8893377E-5	45.0	1
CGGTCTA	80	0.0	45.0	31
TACGAAT	170	0.0	41.02941	12
TAGCACG	45	1.9264917E-8	40.000004	1
CGAATAT	175	0.0	39.857143	14
CGATTGA	40	3.455807E-7	39.375	10
ATGTACG	40	3.455807E-7	39.375	1
TATACGG	40	3.455807E-7	39.375	2
GCCGATA	35	6.2450126E-6	38.57143	9
ACGTATG	35	6.2450126E-6	38.57143	1
CCTATGC	160	0.0	37.96875	35
GCTACGA	185	0.0	37.7027	10
CAGGTCG	30	1.1394954E-4	37.499996	1
CGGGAAC	120	0.0	37.499996	6
AATAGCG	30	1.1394954E-4	37.499996	1
GACCTAT	165	0.0	36.81818	33