##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718022
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34247000788277	33.0	31.0	34.0	30.0	34.0
2	32.456330028884906	34.0	31.0	34.0	31.0	34.0
3	32.5458063959043	34.0	31.0	34.0	31.0	34.0
4	36.03748770929025	37.0	35.0	37.0	35.0	37.0
5	35.99551267231366	37.0	35.0	37.0	35.0	37.0
6	36.01408452665796	37.0	35.0	37.0	35.0	37.0
7	36.148889588341305	37.0	35.0	37.0	35.0	37.0
8	36.13122856959814	37.0	36.0	37.0	35.0	37.0
9	37.671852673037876	39.0	38.0	39.0	35.0	39.0
10	37.508739286539964	39.0	37.0	39.0	35.0	39.0
11	37.442193971772454	39.0	37.0	39.0	35.0	39.0
12	37.316558545559886	39.0	37.0	39.0	35.0	39.0
13	37.2487667508795	39.0	37.0	39.0	34.0	39.0
14	38.465172376333875	40.0	38.0	41.0	34.0	41.0
15	38.5262693900744	40.0	38.0	41.0	34.0	41.0
16	38.543508416176664	40.0	38.0	41.0	34.0	41.0
17	38.54515739072062	40.0	38.0	41.0	34.0	41.0
18	38.484769268908195	40.0	38.0	41.0	34.0	41.0
19	38.4352624292849	40.0	37.0	41.0	34.0	41.0
20	38.33092161521513	40.0	37.0	41.0	34.0	41.0
21	38.266200757079865	40.0	37.0	41.0	34.0	41.0
22	38.24047174042021	40.0	37.0	41.0	34.0	41.0
23	38.20597697563584	40.0	37.0	41.0	34.0	41.0
24	38.18233703145586	40.0	37.0	41.0	34.0	41.0
25	38.123660556361784	40.0	37.0	41.0	34.0	41.0
26	38.00354167421054	40.0	36.0	41.0	34.0	41.0
27	37.9290035681358	40.0	36.0	41.0	34.0	41.0
28	37.83018347627232	40.0	36.0	41.0	33.0	41.0
29	37.743945171596415	40.0	36.0	41.0	33.0	41.0
30	37.71610897716226	40.0	36.0	41.0	33.0	41.0
31	37.6023534097841	40.0	36.0	41.0	33.0	41.0
32	37.39245037060146	40.0	35.0	41.0	33.0	41.0
33	37.28105406241034	40.0	35.0	41.0	32.0	41.0
34	37.0735047115548	39.0	35.0	41.0	31.0	41.0
35	37.030049218547624	39.0	35.0	41.0	31.0	41.0
36	36.93231962251853	39.0	35.0	41.0	31.0	41.0
37	36.864064053747654	39.0	35.0	41.0	31.0	41.0
38	36.75439610485473	39.0	35.0	41.0	31.0	41.0
39	36.670719003038904	39.0	35.0	41.0	31.0	41.0
40	36.62130826074967	39.0	35.0	41.0	31.0	41.0
41	36.5447381835097	39.0	35.0	41.0	31.0	41.0
42	36.494014110988246	39.0	35.0	41.0	31.0	41.0
43	36.41445944553231	39.0	35.0	41.0	30.0	41.0
44	36.19581851252469	38.0	35.0	41.0	30.0	41.0
45	36.12006874441173	38.0	35.0	41.0	30.0	41.0
46	36.02567609349017	38.0	35.0	40.0	30.0	41.0
47	35.94658102397977	38.0	35.0	40.0	30.0	41.0
48	35.81682176869232	38.0	35.0	40.0	29.0	41.0
49	35.716824832665296	38.0	35.0	40.0	29.0	41.0
50	35.64292737548432	38.0	35.0	40.0	29.0	41.0
51	34.35187361947127	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	2.0
13	14.0
14	11.0
15	27.0
16	41.0
17	58.0
18	162.0
19	313.0
20	515.0
21	802.0
22	1280.0
23	1886.0
24	2779.0
25	3997.0
26	5357.0
27	6612.0
28	7589.0
29	8771.0
30	11007.0
31	13300.0
32	17264.0
33	24061.0
34	44241.0
35	58376.0
36	54519.0
37	80937.0
38	138429.0
39	235458.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87587566954773	21.56549520766773	24.333098428738953	12.225530694045586
2	33.01068769480601	25.030291550955262	24.749241666689876	17.20977908754885
3	32.429507730960886	24.459835492505803	26.530941948853936	16.579714827679375
4	29.560654130374836	25.88708981061862	25.545456824442702	19.006799234563843
5	25.68347487959979	30.333193133358034	24.33532677271727	19.64800521432491
6	27.159752765235606	34.74684619691319	23.316695031628555	14.77670600622265
7	79.90270493104669	6.754249869781148	8.430521627471025	4.912523571701145
8	80.6691159880895	5.700382439535279	8.767001568197074	4.863500004178145
9	73.46125884722196	8.48455896894524	11.019718058778142	7.034464125054664
10	41.84077925188922	24.95898454364908	17.75934442120158	15.440891783260122
11	31.49332471706995	24.51596190645969	23.5879959109888	20.402717465481558
12	28.214177281476054	23.27686338301612	27.91711117486651	20.59184816064132
13	24.834893638356483	23.949823264468222	30.01774318892736	21.197539908247936
14	21.63610585748069	26.242371403661725	29.508566589881646	22.61295614897594
15	21.408118414199006	24.919431438033932	32.21057293509112	21.461877212675933
16	23.881580230132222	24.249396258053373	30.177209054875753	21.691814456938648
17	23.877262813674232	24.849099331218262	28.099417566592667	23.17422028851484
18	25.372899437621687	24.100375754503347	28.925993911050078	21.60073089682489
19	25.177919339518844	25.747957583472374	26.98663829242001	22.087484784588774
20	26.985384848932203	25.064413068123258	27.00599703073165	20.944205052212887
21	25.34727348187103	26.241814317667146	27.11031138321667	21.300600817245154
22	24.233240764210567	24.26555175189618	26.558656977084265	24.942550506808985
23	23.806652163861276	25.66926918673801	27.325068034127085	23.199010615273625
24	24.282403603232215	23.801777661408703	28.67043071103671	23.245388024322374
25	23.588692268482024	26.01229488790037	26.43902275974831	23.959990083869297
26	23.21906571107849	26.28345649576197	26.89861870527644	23.5988590878831
27	23.758603496828787	26.158251418480216	27.062262716184183	23.020882368506815
28	22.376751687274208	26.774527799983844	27.66711883479893	23.181601677943014
29	23.319480461601454	25.928314174217505	27.199584413848044	23.552620950332997
30	24.981407254930907	25.39573996339945	26.818955408051565	22.803897373618078
31	25.980958800705274	26.46854831746103	24.77152510647306	22.77896777536064
32	25.291007796418498	26.50085930514664	25.947255098033207	22.26087780040166
33	26.20797134349644	25.715368052789465	25.058145850684244	23.01851475302985
34	23.67949728559849	26.761575550609866	27.186353621476776	22.372573542314857
35	24.018205570302857	25.837369885602392	27.091927545395546	23.052496998699205
36	25.03558386790377	27.97560520429736	25.096584784310227	21.89222614348864
37	24.869850784516352	27.721991805265016	26.806978059168102	20.601179351050526
38	23.893418307517038	26.521332215447437	26.883438111924146	22.701811365111375
39	24.63838155376855	24.927509184955333	27.146939787360274	23.287169473915842
40	23.944530947519713	25.36412533320706	28.285484288782236	22.405859430490988
41	21.84863416441279	26.131511290740395	26.77578124347165	25.24407330137517
42	23.00793011913284	25.644896674475152	27.4336998030701	23.913473403321902
43	24.27975744475796	23.960547169863876	27.756252593931663	24.003442791446503
44	23.8399380520374	24.52111495190955	26.643891134254936	24.995055861798107
45	23.80623434936534	24.58295149730788	27.129252307032374	24.481561846294404
46	23.62225669965544	25.791271019550933	26.5618602215531	24.024612059240525
47	23.080212026929537	25.184743642952444	28.31988434894753	23.415159981170493
48	22.40711287397879	24.32794538328909	28.92404411006905	24.340897632663065
49	23.452902557303258	24.166808259356955	28.427541217400027	23.952747965939764
50	21.920916072209486	24.298698368573664	28.785608240416032	24.994777318800818
51	22.381626189726777	23.847458712964226	27.232173944530945	26.53874115277805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	792.5
2	1289.0
3	2982.5
4	4676.0
5	3163.5
6	1651.0
7	1613.5
8	1576.0
9	1581.5
10	1587.0
11	1647.0
12	1707.0
13	1744.5
14	1782.0
15	1726.0
16	1670.0
17	1691.0
18	1712.0
19	1716.5
20	1721.0
21	1905.0
22	2089.0
23	2417.5
24	2746.0
25	3226.5
26	4164.0
27	4621.0
28	6271.0
29	7921.0
30	8995.5
31	10070.0
32	11447.5
33	12825.0
34	15182.5
35	17540.0
36	18182.5
37	18825.0
38	20600.5
39	22376.0
40	25366.0
41	28356.0
42	31464.5
43	34573.0
44	38887.5
45	43202.0
46	48979.5
47	54757.0
48	58925.0
49	63093.0
50	63858.5
51	64624.0
52	58402.0
53	52180.0
54	47599.5
55	43019.0
56	40972.5
57	38926.0
58	37361.0
59	35796.0
60	35247.0
61	34698.0
62	31754.5
63	28811.0
64	25987.0
65	23163.0
66	19874.5
67	16586.0
68	14093.5
69	11601.0
70	10143.5
71	8686.0
72	7067.0
73	5448.0
74	4336.5
75	2795.5
76	2366.0
77	1807.5
78	1249.0
79	912.5
80	576.0
81	452.5
82	329.0
83	270.5
84	212.0
85	142.5
86	73.0
87	59.0
88	45.0
89	30.5
90	16.0
91	11.0
92	6.0
93	7.5
94	9.0
95	6.0
96	3.0
97	4.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	718022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.61196737241404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28375702841961	22.85031768737905
2	9.138264455118833	5.7775703559141505
3	3.1858889660457357	3.021366541403151
4	1.838087677409441	2.3242227074361423
5	1.2736644924914733	2.0131520190021375
6	0.985310761325144	1.8688566983219332
7	0.9021286631396733	1.9962643305423595
8	0.8078822969759296	2.0430999050203202
9	0.7524938848160285	2.140903092127085
>10	8.711656072026143	50.66088863254298
>50	0.07969167181766137	1.7302777649991743
>100	0.03718944682582005	2.1827559662278815
>500	0.002213657549155956	0.4894266231020071
>1k	0.0017709260393247643	0.9008976759815898
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2622	0.36516986944689717	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1426	0.19860115706761072	Illumina PCR Primer Index 5 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1275	0.17757116077223262	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1114	0.15514844949040557	RNA PCR Primer, Index 33 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	988	0.13760024066114965	Illumina PCR Primer Index 5 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15403427750124649	0.0
2	0.0	0.0	0.0	0.6565258446120036	0.0
3	0.0	0.0	0.0	0.9648729426117862	0.0
4	0.0	0.0	0.0	1.2686240811562877	0.0
5	0.0	0.0	0.0	2.223330204366997	0.0
6	0.0	0.0	0.0	2.990576890401687	0.0
7	0.0	0.0	0.0	3.6991902755068784	0.0
8	0.0	0.0	0.0	4.822554183576548	0.0
9	0.0	0.0	0.0	5.341897602023336	0.0
10	0.0	0.0	0.0	6.275573728938667	0.0
11	0.0	0.0	0.0	7.486678681154617	0.0
12	0.0	0.0	0.0	8.431357256462894	0.0
13	0.0	0.0	0.0	8.844854335939567	0.0
14	0.0	0.0	0.0	9.029110528646754	0.0
15	0.0	0.0	0.0	9.353891663486634	0.0
16	0.0	0.0	0.0	9.967104072020078	0.0
17	0.0	0.0	0.0	10.666107723718772	0.0
18	0.0	0.0	0.0	11.410096069479765	0.0
19	0.0	0.0	0.0	11.885986780349349	0.0
20	0.0	0.0	0.0	12.347950341354442	0.0
21	1.3927149864488832E-4	0.0	0.0	12.915063883836428	0.0
22	1.3927149864488832E-4	0.0	0.0	13.501118350134119	0.0
23	1.3927149864488832E-4	0.0	0.0	14.096364735342371	0.0
24	1.3927149864488832E-4	0.0	0.0	14.592310542016818	0.0
25	1.3927149864488832E-4	0.0	0.0	15.025584174301066	0.0
26	1.3927149864488832E-4	0.0	0.0	15.417494171487782	0.0
27	2.7854299728977664E-4	0.0	0.0	15.82973780747665	0.0
28	2.7854299728977664E-4	0.0	0.0	16.255769321831366	0.0
29	2.7854299728977664E-4	0.0	0.0	16.689182225614257	0.0
30	2.7854299728977664E-4	0.0	0.0	17.191395249727723	0.0
31	2.7854299728977664E-4	0.0	0.0	17.634696429914403	0.0
32	2.7854299728977664E-4	0.0	0.0	18.106548267323284	0.0
33	2.7854299728977664E-4	0.0	0.0	18.5543061354666	0.0
34	2.7854299728977664E-4	0.0	0.0	18.98702268175627	0.0
35	2.7854299728977664E-4	0.0	0.0	19.44341538281557	0.0
36	2.7854299728977664E-4	0.0	0.0	19.87334649913234	0.0
37	2.7854299728977664E-4	0.0	0.0	20.313165891852897	0.0
38	2.7854299728977664E-4	0.0	0.0	20.77053349340271	0.0
39	2.7854299728977664E-4	0.0	0.0	21.1999075237249	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.031516E-4	45.0	1
CGACGGT	80	0.0	45.0	28
GCAAACG	20	7.031516E-4	45.0	1
TCGATTG	20	7.031516E-4	45.0	1
TATTGCG	25	3.8893377E-5	45.0	1
CGGTCTA	80	0.0	45.0	31
TACGAAT	170	0.0	41.02941	12
TAGCACG	45	1.9264917E-8	40.000004	1
CGAATAT	175	0.0	39.857143	14
CGATTGA	40	3.455807E-7	39.375	10
ATGTACG	40	3.455807E-7	39.375	1
TATACGG	40	3.455807E-7	39.375	2
GCCGATA	35	6.2450126E-6	38.57143	9
ACGTATG	35	6.2450126E-6	38.57143	1
CCTATGC	160	0.0	37.96875	35
GCTACGA	185	0.0	37.7027	10
CAGGTCG	30	1.1394954E-4	37.499996	1
CGGGAAC	120	0.0	37.499996	6
AATAGCG	30	1.1394954E-4	37.499996	1
GACCTAT	165	0.0	36.81818	33
>>END_MODULE