SRR3550454_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704558
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

$Per Sequence quality graph$

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4199	0.595976484547759	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	2323	0.3297102580625016	RNA PCR Primer, Index 41 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	2219	0.3149492305814426	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1747	0.24795687509048225	RNA PCR Primer, Index 41 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	955	0.13554597350395567	Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTG	854	0.12121074489254255	RNA PCR Primer, Index 41 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.8892627E-5	45.000004	1
TCGCACG	25	3.8892627E-5	45.000004	1
TAGCGCG	25	3.8892627E-5	45.000004	1
GCGATAC	25	3.8892627E-5	45.000004	9
CGACCCG	20	7.0314255E-4	45.0	32
TACGGGT	45	3.8380676E-10	45.0	4
CTCGCGG	35	1.2110286E-7	45.0	2
CGGTCTA	20	7.0314255E-4	45.0	31
GTACGAG	35	1.2110286E-7	45.0	1
CGGGTTG	30	2.164301E-6	44.999996	6
TCTACGG	30	2.164301E-6	44.999996	2
TAGGACG	30	2.164301E-6	44.999996	1
TCGCGCG	30	2.164301E-6	44.999996	1
CGTTTTT	2180	0.0	42.110092	1
CTACGAA	115	0.0	41.08696	11
CGACTGG	55	6.002665E-11	40.909092	2
CGAATAT	105	0.0	40.714283	14
GCTACGA	105	0.0	40.714283	10
CTGCGCG	45	1.9264917E-8	40.0	1
CGTAAGG	45	1.9264917E-8	40.0	2