##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704558
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.134468702363755	33.0	31.0	34.0	30.0	34.0
2	32.25210841406953	34.0	31.0	34.0	30.0	34.0
3	32.34027432801842	34.0	31.0	34.0	30.0	34.0
4	35.86053241890661	37.0	35.0	37.0	35.0	37.0
5	35.84663008581266	37.0	35.0	37.0	35.0	37.0
6	35.870588368878074	37.0	35.0	37.0	35.0	37.0
7	36.10204127978108	37.0	35.0	37.0	35.0	37.0
8	36.12239304642059	37.0	35.0	37.0	35.0	37.0
9	37.715207264696446	39.0	38.0	39.0	35.0	39.0
10	37.39583966117765	39.0	37.0	39.0	34.0	39.0
11	37.33116932885582	39.0	37.0	39.0	34.0	39.0
12	37.369316649587404	39.0	37.0	39.0	34.0	39.0
13	37.34921894294011	39.0	37.0	39.0	34.0	39.0
14	38.53924730114483	40.0	38.0	41.0	34.0	41.0
15	38.61473860207393	40.0	38.0	41.0	34.0	41.0
16	38.616234575435946	40.0	38.0	41.0	34.0	41.0
17	38.63390380919669	40.0	38.0	41.0	35.0	41.0
18	38.57397971494185	40.0	38.0	41.0	35.0	41.0
19	38.53286315675928	40.0	38.0	41.0	34.0	41.0
20	38.40256160599979	40.0	37.0	41.0	34.0	41.0
21	38.37267904132804	40.0	37.0	41.0	34.0	41.0
22	38.35279423411557	40.0	37.0	41.0	34.0	41.0
23	38.31975791914931	40.0	37.0	41.0	34.0	41.0
24	38.31980617635454	40.0	37.0	41.0	34.0	41.0
25	38.288622086471236	40.0	37.0	41.0	34.0	41.0
26	38.166854964389024	40.0	37.0	41.0	34.0	41.0
27	38.093672912662974	40.0	37.0	41.0	34.0	41.0
28	38.03312857138802	40.0	37.0	41.0	34.0	41.0
29	37.98134291286168	40.0	37.0	41.0	34.0	41.0
30	37.93170753862705	40.0	37.0	41.0	33.0	41.0
31	37.828588987705764	40.0	37.0	41.0	33.0	41.0
32	37.65528884775987	40.0	37.0	41.0	33.0	41.0
33	37.514539612068845	40.0	36.0	41.0	33.0	41.0
34	37.36115550458585	40.0	36.0	41.0	32.0	41.0
35	37.315516962407635	40.0	36.0	41.0	32.0	41.0
36	37.18771910900167	40.0	36.0	41.0	31.0	41.0
37	37.15283624627071	40.0	36.0	41.0	31.0	41.0
38	37.12149744946477	40.0	36.0	41.0	31.0	41.0
39	37.06797169289115	40.0	36.0	41.0	31.0	41.0
40	37.024057636135	40.0	36.0	41.0	31.0	41.0
41	36.92415244734997	39.0	36.0	41.0	31.0	41.0
42	36.85561869995089	39.0	35.0	41.0	31.0	41.0
43	36.76750672052549	39.0	35.0	41.0	31.0	41.0
44	36.59685930753749	39.0	35.0	41.0	30.0	41.0
45	36.57555375142997	39.0	35.0	41.0	30.0	41.0
46	36.49985806704345	39.0	35.0	41.0	30.0	41.0
47	36.41146222170496	39.0	35.0	41.0	30.0	41.0
48	36.29815146517391	39.0	35.0	41.0	29.0	41.0
49	36.22299086803358	39.0	35.0	41.0	29.0	41.0
50	36.14739879470533	39.0	35.0	40.0	29.0	41.0
51	34.878712611310924	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	4.0
12	2.0
13	5.0
14	8.0
15	5.0
16	12.0
17	54.0
18	80.0
19	172.0
20	362.0
21	636.0
22	990.0
23	1593.0
24	2573.0
25	3957.0
26	5434.0
27	6915.0
28	7567.0
29	8683.0
30	10653.0
31	13276.0
32	16733.0
33	21979.0
34	34543.0
35	47603.0
36	50175.0
37	73949.0
38	139610.0
39	256806.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.69702707229214	22.2694228154389	26.57353972277655	12.46001038949242
2	31.75537003341102	24.276638686949834	26.667925138881397	17.300066140757753
3	30.325963228009616	24.251090754771077	29.141816571524277	16.28112944569503
4	27.213657356810938	26.353827506039245	28.702676003962768	17.72983913318705
5	24.34618583565867	30.540140059441523	26.028233303716657	19.085440801183154
6	25.9557055629203	34.208255388484694	24.59513624144499	15.240902807150015
7	81.57454744676804	4.839629952395686	9.007917020316283	4.577905580519985
8	82.7108626968965	3.7945775933280155	8.517538655440717	4.977021054334775
9	75.5795832280664	7.602780750484701	11.205181120645852	5.612454900803057
10	32.432816035017694	39.41549169834137	16.602607592277714	11.549084674363218
11	21.425063656931012	23.661927052137653	36.200710232514574	18.712299058416768
12	21.980731181818953	22.03196897913302	36.31695332392791	19.67034651512012
13	21.092940538607184	23.47258848810176	37.76112115681037	17.673349816480687
14	18.31687384147224	27.777556993178703	33.47715872930262	20.42841043604643
15	17.55029394315301	27.541664419394856	36.108311877801405	18.79972975965073
16	20.91637594066067	26.60263597886902	33.35935437536725	19.121633705103058
17	20.315999534459902	26.606893967565483	31.13838747129406	21.938719026680555
18	20.007011488053504	25.810224282457938	33.52499013565952	20.65777409382904
19	20.057823486497917	27.747467206390393	31.608185557470076	20.586523749641618
20	21.818785678396953	27.17462579376006	31.417711529781794	19.588876998061195
21	21.247789394201753	28.199381740041275	31.696467856443327	18.85636100931364
22	19.775518835922664	25.338155268977147	31.234050284007846	23.652275611092342
23	18.141870506047763	27.713829095688357	31.585476284422292	22.558824113841585
24	19.983450617266428	26.020143125193385	32.138447083135816	21.857959174404378
25	19.314520593052666	27.745764010911806	30.38429767315111	22.555417722884417
26	19.939025601866703	28.027358996704315	30.766381192180063	21.26723420924892
27	20.8192937983814	26.41812313535578	31.76573113923907	20.99685192702375
28	18.923211431848053	26.91446268440639	32.156046769747846	22.006279113997714
29	21.19697739575734	24.92300137107236	31.082323953457347	22.797697279712956
30	22.645119351423162	24.937194666727226	30.902494897510213	21.515191084339403
31	22.493676886785757	27.165400151584397	28.5478271483682	21.793095813261647
32	22.109464373408578	27.997127276959453	29.104488203951984	20.788920145679988
33	21.844617476488807	26.514353679895763	29.266575640330533	22.374453203284897
34	21.450895455022863	26.74840112524448	29.460314126019433	22.340389293713223
35	20.506473562148184	26.252913173933162	30.431845213594904	22.80876805032375
36	22.682731584908552	25.72336131305017	30.364427059234302	21.22948004280698
37	21.66209169436725	27.82893672344931	30.870843848199865	19.638127733983577
38	20.575452979030825	27.751157463260657	29.579537809520296	22.093851748188225
39	22.142108953414766	26.4528967097102	28.633838520036676	22.77115581683836
40	21.613550623227614	25.198777105646375	30.998725442050194	22.188946829075817
41	20.246878184620712	26.287119016461386	30.345266110100233	23.12073668881767
42	23.186025848830045	24.839970591491404	30.945500583344455	21.0285029763341
43	23.429157003397876	24.324753959219823	30.87453410507013	21.371554932312172
44	21.65357571697433	25.812779075675813	29.220589362408774	23.313055844941083
45	21.468920940504542	24.6730574345902	29.34676776078052	24.51125386412474
46	22.911527510864968	24.9137757288967	29.664413717536387	22.51028304270195
47	20.388101476386613	24.71790824885957	31.944850530403457	22.949139744350358
48	20.734701756278405	23.824298354429303	31.513658208408675	23.927341680883618
49	22.04190428609142	23.104698264727674	31.87218085665055	22.98121659253035
50	20.608949156776305	23.454137203750435	31.26414007079616	24.6727735686771
51	19.79141532705611	22.51113464044124	30.057568007176126	27.639882025326518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	397.0
2	614.0
3	3470.0
4	6326.0
5	4116.5
6	1907.0
7	1860.0
8	1813.0
9	1845.0
10	1877.0
11	1902.5
12	1928.0
13	1959.0
14	1990.0
15	1941.0
16	1892.0
17	1916.5
18	1941.0
19	2029.5
20	2118.0
21	2515.0
22	2912.0
23	3405.0
24	3898.0
25	4727.5
26	6835.5
27	8114.0
28	9333.5
29	10553.0
30	12252.5
31	13952.0
32	15911.0
33	17870.0
34	20517.0
35	23164.0
36	25217.0
37	27270.0
38	29508.5
39	31747.0
40	34700.0
41	37653.0
42	41813.0
43	45973.0
44	50554.5
45	55136.0
46	63901.0
47	72666.0
48	77509.5
49	82353.0
50	78032.0
51	73711.0
52	61483.0
53	49255.0
54	41243.0
55	33231.0
56	28551.0
57	23871.0
58	21524.0
59	19177.0
60	17184.5
61	15192.0
62	13167.0
63	11142.0
64	9194.5
65	7247.0
66	5591.0
67	3935.0
68	3208.0
69	2481.0
70	1986.0
71	1491.0
72	1234.0
73	977.0
74	798.0
75	507.0
76	395.0
77	301.0
78	207.0
79	155.5
80	104.0
81	92.0
82	80.0
83	51.0
84	22.0
85	17.5
86	13.0
87	7.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	704558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.747314175049894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21004791472161	26.535253173176816
2	10.64205392596373	7.821337981704247
3	3.839028462196222	4.232219550818497
4	2.05516597206516	3.02087318629401
5	1.3010545488248018	2.3905130132271393
6	1.0492778681999428	2.313488608780194
7	0.8955142424901409	2.303542025191191
8	0.7765291362683274	2.282828810922589
9	0.6648951039073995	2.198979834906389
>10	6.4711698977032235	41.19546491124847
>50	0.061434867928982236	1.5537200559243813
>100	0.029162120457308927	1.8650641601133444
>500	0.0031106261821129497	0.7881478516191834
>1k	0.0015553130910564749	1.4985668360736035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4199	0.595976484547759	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	2323	0.3297102580625016	RNA PCR Primer, Index 41 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	2219	0.3149492305814426	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1747	0.24795687509048225	RNA PCR Primer, Index 41 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	955	0.13554597350395567	Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTG	854	0.12121074489254255	RNA PCR Primer, Index 41 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2798917903139273	0.0
2	0.0	0.0	0.0	1.2356683197125007	0.0
3	0.0	0.0	0.0	1.7437883041566486	0.0
4	0.0	0.0	0.0	2.3636095254045797	0.0
5	0.0	0.0	0.0	4.0391280774613305	0.0
6	0.0	0.0	0.0	5.217029683858533	0.0
7	0.0	0.0	0.0	6.362712509119192	0.0
8	0.0	0.0	0.0	8.306058550183236	0.0
9	0.0	0.0	0.0	9.107270089900334	0.0
10	0.0	0.0	0.0	10.538805889649964	0.0
11	0.0	0.0	0.0	12.39188257034907	0.0
12	0.0	0.0	0.0	13.902191160983199	0.0
13	0.0	0.0	0.0	14.517470527621573	0.0
14	0.0	0.0	0.0	14.789414072368777	0.0
15	0.0	0.0	0.0	15.1848392893133	0.0
16	0.0	0.0	0.0	16.011740694165702	0.0
17	0.0	0.0	0.0	17.207525853088036	0.0
18	0.0	0.0	0.0	18.403169079053818	0.0
19	0.0	0.0	0.0	19.161942664762872	0.0
20	0.0	0.0	0.0	19.871039715679903	0.0
21	0.0	0.0	0.0	20.718947198101503	0.0
22	0.0	0.0	0.0	21.65258218627849	0.0
23	0.0	0.0	0.0	22.58124952097627	0.0
24	0.0	0.0	0.0	23.275017812586047	0.0
25	0.0	0.0	0.0	23.897393827051854	0.0
26	0.0	0.0	0.0	24.492092914990675	0.0
27	0.0	0.0	0.0	25.06763105379543	0.0
28	0.0	0.0	0.0	25.675246040780177	0.0
29	0.0	0.0	0.0	26.309828289509166	0.0
30	0.0	0.0	0.0	27.071156668436096	0.0
31	0.0	0.0	0.0	27.734409374387916	0.0
32	0.0	0.0	0.0	28.332372920327355	0.0
33	0.0	0.0	0.0	28.926078477570336	0.0
34	0.0	0.0	0.0	29.51510024724721	0.0
35	1.4193295654864468E-4	0.0	0.0	30.132366675277265	0.0
36	1.4193295654864468E-4	0.0	0.0	30.730472154173256	0.0
37	1.4193295654864468E-4	0.0	0.0	31.337803275244905	0.0
38	1.4193295654864468E-4	0.0	0.0	31.91362527996276	0.0
39	1.4193295654864468E-4	0.0	0.0	32.479511977722204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.8892627E-5	45.000004	1
TCGCACG	25	3.8892627E-5	45.000004	1
TAGCGCG	25	3.8892627E-5	45.000004	1
GCGATAC	25	3.8892627E-5	45.000004	9
CGACCCG	20	7.0314255E-4	45.0	32
TACGGGT	45	3.8380676E-10	45.0	4
CTCGCGG	35	1.2110286E-7	45.0	2
CGGTCTA	20	7.0314255E-4	45.0	31
GTACGAG	35	1.2110286E-7	45.0	1
CGGGTTG	30	2.164301E-6	44.999996	6
TCTACGG	30	2.164301E-6	44.999996	2
TAGGACG	30	2.164301E-6	44.999996	1
TCGCGCG	30	2.164301E-6	44.999996	1
CGTTTTT	2180	0.0	42.110092	1
CTACGAA	115	0.0	41.08696	11
CGACTGG	55	6.002665E-11	40.909092	2
CGAATAT	105	0.0	40.714283	14
GCTACGA	105	0.0	40.714283	10
CTGCGCG	45	1.9264917E-8	40.0	1
CGTAAGG	45	1.9264917E-8	40.0	2
>>END_MODULE