FastQCFastQC Report
Sat 18 Jun 2016
SRR3550452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295002
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC18650.6321991037348899TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC17770.602368797499678TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT13340.4522003240655995TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC7300.24745594945119018TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC5640.19118514450749488TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.18949024074413054No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG4850.1644056650463387TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG3600.12203307096223077TruSeq Adapter, Index 16 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG3360.11389753289808205TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG3230.10949078311333484TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC3110.10542301408126047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT600.045.00000441
ACAACGG207.024753E-445.02
TCCGCGG207.024753E-445.02
ATCTATG207.024753E-445.026
CAACGAT207.024753E-445.014
GGGTCTA207.024753E-445.08
GAATCTT207.024753E-445.09
ACGGGTC253.883734E-545.05
TAGTTAG253.883734E-545.01
GTCACGG253.883734E-545.02
GAAACTC207.024753E-445.018
TTAACGG253.883734E-545.02
TACTACA207.024753E-445.039
TTTGACG207.024753E-445.01
ACCCCAT207.024753E-445.032
ATCATAA207.024753E-445.040
AACAGCG253.883734E-545.036
CGTAAGG551.8189894E-1244.9999962
ACGACCA650.044.99999628
TCAAGCG800.042.187517
Produced by FastQC (version 0.11.5)