##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295002
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.992711913817534	33.0	31.0	34.0	30.0	34.0
2	32.10891451583379	33.0	31.0	34.0	30.0	34.0
3	32.21621209347733	34.0	31.0	34.0	30.0	34.0
4	35.75798130182168	37.0	35.0	37.0	33.0	37.0
5	35.74559833492654	37.0	35.0	37.0	35.0	37.0
6	35.75745588165504	37.0	35.0	37.0	35.0	37.0
7	35.979498444078345	37.0	35.0	37.0	35.0	37.0
8	35.96584429936068	37.0	35.0	37.0	35.0	37.0
9	37.57548762381272	39.0	37.0	39.0	35.0	39.0
10	37.304875899146445	39.0	37.0	39.0	34.0	39.0
11	37.253686415685316	39.0	37.0	39.0	34.0	39.0
12	37.06760632131308	39.0	37.0	39.0	34.0	39.0
13	36.90492606829784	39.0	37.0	39.0	33.0	39.0
14	37.98206452837608	40.0	37.0	41.0	33.0	41.0
15	38.08513840584131	40.0	37.0	41.0	33.0	41.0
16	38.15140914298886	40.0	37.0	41.0	34.0	41.0
17	38.189805492844116	40.0	37.0	41.0	34.0	41.0
18	38.14798204757934	40.0	37.0	41.0	34.0	41.0
19	38.08798584416377	40.0	37.0	41.0	34.0	41.0
20	37.951369821221554	40.0	36.0	41.0	34.0	41.0
21	37.866000908468415	40.0	36.0	41.0	33.0	41.0
22	37.78809296208161	39.0	36.0	41.0	33.0	41.0
23	37.65067355475556	39.0	35.0	41.0	33.0	41.0
24	37.58813160588742	39.0	35.0	41.0	33.0	41.0
25	37.43085809587732	39.0	35.0	41.0	33.0	41.0
26	37.14986000094915	39.0	35.0	41.0	32.0	41.0
27	37.018542247171204	39.0	35.0	41.0	31.0	41.0
28	36.797404763357534	39.0	35.0	41.0	31.0	41.0
29	36.74821187652965	39.0	35.0	41.0	31.0	41.0
30	36.69195124100854	39.0	35.0	41.0	31.0	41.0
31	36.43049199666443	38.0	35.0	40.0	30.0	41.0
32	36.27724218818855	38.0	35.0	40.0	30.0	41.0
33	36.14387360085694	38.0	35.0	40.0	30.0	41.0
34	36.06773174419156	38.0	35.0	40.0	30.0	41.0
35	36.14671086975682	38.0	35.0	40.0	30.0	41.0
36	36.041613277198124	38.0	35.0	40.0	30.0	41.0
37	36.00264066006332	38.0	35.0	40.0	30.0	41.0
38	35.95722740862774	38.0	35.0	40.0	30.0	41.0
39	35.86857038257368	38.0	35.0	40.0	29.0	41.0
40	35.793872583914684	38.0	35.0	40.0	29.0	41.0
41	35.64727357780625	38.0	35.0	40.0	28.0	41.0
42	35.58802652185409	38.0	35.0	40.0	28.0	41.0
43	35.5008745703419	38.0	35.0	40.0	28.0	41.0
44	35.31407583677399	38.0	34.0	40.0	27.0	41.0
45	35.307957234188244	38.0	34.0	40.0	27.0	41.0
46	35.185869926305585	38.0	34.0	40.0	27.0	41.0
47	35.124243225469655	37.0	34.0	40.0	26.0	41.0
48	35.07476559480953	37.0	34.0	40.0	26.0	41.0
49	35.03118283943838	37.0	34.0	40.0	26.0	41.0
50	34.906437922454764	37.0	34.0	40.0	26.0	41.0
51	33.36565514810069	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	1.0
14	2.0
15	9.0
16	17.0
17	34.0
18	66.0
19	95.0
20	250.0
21	417.0
22	699.0
23	1005.0
24	1562.0
25	2219.0
26	2824.0
27	3371.0
28	4062.0
29	4875.0
30	5906.0
31	7561.0
32	9762.0
33	12940.0
34	20556.0
35	29222.0
36	25466.0
37	38043.0
38	61120.0
39	62868.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89530918434451	21.236466193449534	25.01135585521454	11.85686876699141
2	31.616056840292607	24.064921593751905	25.410675181863173	18.90834638409231
3	30.472674761527042	24.191361414498886	27.724896780360815	17.611067043613264
4	28.610314506342334	26.705581657073513	25.30220134100786	19.381902495576302
5	24.51610497555949	29.878780482844185	25.966942596999342	19.638171944596987
6	25.465251083043505	33.297062392797336	25.881180466573106	15.35650605758605
7	77.1967647676965	6.519616816157178	10.248066114806	6.03555230134033
8	75.53203029132007	5.570470708673161	11.038230249286446	7.859268750720334
9	69.61478227266255	7.203340994298343	14.414139565155489	8.767737167883608
10	38.62041613277198	23.815092779032007	21.357482322153746	16.207008766042264
11	28.26353719635799	23.90526165924299	25.955417251408463	21.875783892990555
12	25.194405461657887	20.862231442498693	32.66452430830977	21.278838787533644
13	23.08865702605406	24.596443413942957	33.971634090616334	18.34326546938665
14	18.895804096243417	28.952346085789248	30.766910054847084	21.38493976312025
15	17.217171409007396	23.673737805167423	39.10651453210487	20.002576253720314
16	19.146988833974007	24.98288147199002	32.81672666625989	23.05340302777608
17	19.07275204913865	24.594748510179592	28.091334974000176	28.241164466681585
18	21.900190507183	23.99305767418526	31.97063070758842	22.136121111043312
19	22.314763967701914	26.337787540423456	28.10963993464451	23.23780855723012
20	23.110351794225124	26.078806245381386	28.978108622992387	21.832733337401102
21	21.10087389238039	26.859817899539664	30.502505067762254	21.53680314031769
22	19.144954949457972	25.282201476600157	27.347950183388587	28.224893390553284
23	18.92563440247863	26.62693812245341	27.86828563874143	26.57914183632653
24	22.259510105016236	22.743913600585756	30.142168527670997	24.854407766727004
25	19.86495006813513	25.679486918732753	28.413705669792066	26.041857343340048
26	19.047667473440857	26.760157558253844	30.420132744862748	23.77204222344255
27	20.89816340228202	26.180500471183247	28.905905722673065	24.015430403861668
28	17.700218981566227	24.522545609860273	32.25367963607027	25.523555772503236
29	19.28224215429048	21.99544409868408	31.51809140277015	27.204222344255292
30	20.910705690130914	23.747296628497434	29.47335950264744	25.86863817872421
31	19.969695120711044	25.593385807553847	28.08489433969939	26.352024732035716
32	19.604951830835045	24.475766265991417	28.37980759452478	27.539474308648753
33	18.966651073552043	22.85679419122582	28.633365197524085	29.54318953769805
34	17.295136982122155	21.44968508688077	32.48893227842523	28.766245652571847
35	16.87920759859255	20.513081267245646	32.762150765079554	29.845560369082243
36	19.881899105768774	20.849689154649802	31.992325475759486	27.27608626382194
37	17.6093721398499	22.579168954786745	31.8984278072691	27.913031098094248
38	17.554796238669567	23.666619209361293	30.395048169164955	28.383536382804188
39	19.936475006949106	20.66155483691636	30.637758388078723	28.76421176805581
40	20.65918197164765	19.720883248249162	33.514349055260645	26.105585724842545
41	17.231747581372332	20.810028406587076	30.235388234656035	31.72283577738456
42	20.23782889607528	19.8341028196419	31.27300831858767	28.655059965695145
43	21.295787825167288	19.396139687188562	31.440803791160736	27.867268696483414
44	19.38529230310303	20.81782496389855	31.14487359407733	28.65200913892109
45	19.95105117931404	19.564952102019646	30.26284567562254	30.221151043043776
46	22.325611351787444	20.964264649053227	30.717079884204175	25.993044114955154
47	17.58462654490478	20.917824285937044	34.254683019098174	27.242866150060003
48	18.27953708788415	18.805974196785105	33.1051992867845	29.809289428546247
49	19.7866455142677	17.089375665249726	34.98077979132345	28.14319902915912
50	19.024616782259105	18.164961593480722	33.66553447095274	29.144887153307437
51	18.573772381204197	18.80834706205382	28.786245516979548	33.83163503976244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	184.0
1	334.5
2	485.0
3	817.5
4	1150.0
5	825.5
6	501.0
7	521.0
8	541.0
9	585.5
10	630.0
11	690.5
12	751.0
13	820.0
14	889.0
15	887.0
16	885.0
17	921.5
18	958.0
19	949.5
20	941.0
21	907.0
22	873.0
23	895.5
24	918.0
25	1065.0
26	1321.5
27	1431.0
28	1660.0
29	1889.0
30	2258.5
31	2628.0
32	2851.5
33	3075.0
34	3582.0
35	4089.0
36	4921.5
37	5754.0
38	6773.0
39	7792.0
40	9374.0
41	10956.0
42	13503.5
43	16051.0
44	19015.0
45	21979.0
46	27212.5
47	32446.0
48	36691.0
49	40936.0
50	40486.5
51	40037.0
52	32473.0
53	24909.0
54	20553.5
55	16198.0
56	14196.5
57	12195.0
58	11358.0
59	10521.0
60	9754.5
61	8988.0
62	8034.5
63	7081.0
64	6090.0
65	5099.0
66	4398.0
67	3697.0
68	3103.0
69	2509.0
70	2069.5
71	1630.0
72	1240.5
73	851.0
74	763.0
75	524.5
76	374.0
77	264.0
78	154.0
79	111.0
80	68.0
81	44.0
82	20.0
83	24.0
84	28.0
85	18.0
86	8.0
87	7.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	4.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	295002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.58063291720048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.19448264374637	24.619834868427397
2	13.043990479668507	9.28226874065086
3	5.031084309645261	5.37027491990925
4	2.4331962525670408	3.462986507123829
5	1.40935365958606	2.5072847606122366
6	0.9777004236356489	2.0872319925822858
7	0.7185662148922587	1.7896928503148561
8	0.5650849840805047	1.6084865108472413
9	0.5082773490839034	1.6276346800093922
>10	5.994682530437412	40.751502167873596
>50	0.07574328711236981	1.9003254412407269
>100	0.04185813235157279	2.5708825245372773
>500	0.0029898665965409138	0.6570821128231138
>1k	0.0029898665965409138	1.76451192304793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1865	0.6321991037348899	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1777	0.602368797499678	TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	1334	0.4522003240655995	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	730	0.24745594945119018	TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	564	0.19118514450749488	TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.18949024074413054	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	485	0.1644056650463387	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	360	0.12203307096223077	TruSeq Adapter, Index 16 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	336	0.11389753289808205	TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	323	0.10949078311333484	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC	311	0.10542301408126047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3898075267286323E-4	0.0	0.0	0.5172846285787893	0.0
2	6.779615053457265E-4	0.0	0.0	2.412526016772768	0.0
3	6.779615053457265E-4	0.0	0.0	3.405061660598911	0.0
4	6.779615053457265E-4	0.0	0.0	4.377597440017356	0.0
5	6.779615053457265E-4	0.0	0.0	7.167070053762347	0.0
6	6.779615053457265E-4	0.0	0.0	8.684686883478756	0.0
7	6.779615053457265E-4	0.0	0.0	10.352472186629242	0.0
8	6.779615053457265E-4	0.0	0.0	13.111436532633677	0.0
9	6.779615053457265E-4	0.0	0.0	14.135158405705724	0.0
10	6.779615053457265E-4	0.0	0.0	16.285652300662367	0.0
11	6.779615053457265E-4	0.0	0.0	19.10936197042732	0.0
12	6.779615053457265E-4	0.0	0.0	21.300194574952034	0.0
13	6.779615053457265E-4	0.0	0.0	22.192730896739683	0.0
14	6.779615053457265E-4	0.0	0.0	22.498491535650604	0.0
15	6.779615053457265E-4	0.0	0.0	23.14052108121301	0.0
16	6.779615053457265E-4	0.0	0.0	24.480173015776163	0.0
17	6.779615053457265E-4	0.0	0.0	26.132026223551026	0.0
18	6.779615053457265E-4	0.0	0.0	28.065572436797037	0.0
19	6.779615053457265E-4	0.0	0.0	29.012006698259672	0.0
20	6.779615053457265E-4	0.0	0.0	29.895729520477826	0.0
21	6.779615053457265E-4	0.0	0.0	31.03131504193192	0.0
22	6.779615053457265E-4	0.0	0.0	32.072324933390284	0.0
23	6.779615053457265E-4	0.0	0.0	33.11197890183795	0.0
24	6.779615053457265E-4	0.0	0.0	33.94010888061776	0.0
25	6.779615053457265E-4	0.0	0.0	34.69196819004617	0.0
26	6.779615053457265E-4	0.0	0.0	35.365183964854474	0.0
27	6.779615053457265E-4	0.0	0.0	36.016027009986374	0.0
28	6.779615053457265E-4	0.0	0.0	36.664836170602236	0.0
29	6.779615053457265E-4	0.0	0.0	37.33771296465787	0.0
30	6.779615053457265E-4	0.0	0.0	38.15330065558877	0.0
31	6.779615053457265E-4	0.0	0.0	38.83465196846123	0.0
32	6.779615053457265E-4	0.0	0.0	39.425495420370034	0.0
33	6.779615053457265E-4	0.0	0.0	40.04616917851404	0.0
34	6.779615053457265E-4	0.0	0.0	40.63328384214344	0.0
35	6.779615053457265E-4	0.0	0.0	41.313957193510554	0.0
36	6.779615053457265E-4	0.0	0.0	41.90852943369875	0.0
37	6.779615053457265E-4	0.0	0.0	42.54479630646572	0.0
38	6.779615053457265E-4	0.0	0.0	43.0946230873011	0.0
39	6.779615053457265E-4	0.0	0.0	43.71190703791839	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACACGT	60	0.0	45.000004	41
ACAACGG	20	7.024753E-4	45.0	2
TCCGCGG	20	7.024753E-4	45.0	2
ATCTATG	20	7.024753E-4	45.0	26
CAACGAT	20	7.024753E-4	45.0	14
GGGTCTA	20	7.024753E-4	45.0	8
GAATCTT	20	7.024753E-4	45.0	9
ACGGGTC	25	3.883734E-5	45.0	5
TAGTTAG	25	3.883734E-5	45.0	1
GTCACGG	25	3.883734E-5	45.0	2
GAAACTC	20	7.024753E-4	45.0	18
TTAACGG	25	3.883734E-5	45.0	2
TACTACA	20	7.024753E-4	45.0	39
TTTGACG	20	7.024753E-4	45.0	1
ACCCCAT	20	7.024753E-4	45.0	32
ATCATAA	20	7.024753E-4	45.0	40
AACAGCG	25	3.883734E-5	45.0	36
CGTAAGG	55	1.8189894E-12	44.999996	2
ACGACCA	65	0.0	44.999996	28
TCAAGCG	80	0.0	42.1875	17
>>END_MODULE