FastQCFastQC Report
Sat 18 Jun 2016
SRR3550450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606206
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.46271399491262044No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC13400.22104697083169747TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC12940.21345879123598246TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT11030.1819513498711659TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG7320.12075103182746459No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA207.030645E-445.00000414
AATGCGA207.030645E-445.00000425
TCCGTTA207.030645E-445.00000433
CTATGCG253.8886166E-545.01
ACGTAAG302.1637989E-644.9999961
TTGCGAG850.042.352941
CGTAAGG850.042.352942
TGATTCG700.041.78571315
CGTTTTT15600.040.9615361
CACGACC4200.040.71428727
AACACGT4000.039.937541
ACGACCA4300.039.76744528
TTTAGCG403.4546247E-739.3750041
ATTCGTT403.4546247E-739.37500441
ACACGAC4350.039.31034526
ACATACG750.039.00000417
CGACCAA4450.038.93258329
CGAAAGG1100.038.8636362
AAACACG4300.038.72093240
CTAAGCG356.2434065E-638.5714261
Produced by FastQC (version 0.11.5)