##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	606206
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.176982741840234	33.0	31.0	34.0	30.0	34.0
2	32.29186448171084	34.0	31.0	34.0	30.0	34.0
3	32.377153970762414	34.0	31.0	34.0	30.0	34.0
4	35.90130087791939	37.0	35.0	37.0	35.0	37.0
5	35.8754416815406	37.0	35.0	37.0	35.0	37.0
6	35.89604028993445	37.0	35.0	37.0	35.0	37.0
7	36.08328851908427	37.0	35.0	37.0	35.0	37.0
8	36.07829351738518	37.0	35.0	37.0	35.0	37.0
9	37.68526375522512	39.0	38.0	39.0	35.0	39.0
10	37.38315853026859	39.0	37.0	39.0	34.0	39.0
11	37.333256351801204	39.0	37.0	39.0	34.0	39.0
12	37.21116254210615	39.0	37.0	39.0	34.0	39.0
13	37.138325915612846	39.0	37.0	39.0	33.0	39.0
14	38.296153452786676	40.0	37.0	41.0	34.0	41.0
15	38.3609268136574	40.0	37.0	41.0	34.0	41.0
16	38.38296717617443	40.0	37.0	41.0	34.0	41.0
17	38.35968631125393	40.0	37.0	41.0	34.0	41.0
18	38.30962906998611	40.0	37.0	41.0	34.0	41.0
19	38.24334632121754	40.0	37.0	41.0	34.0	41.0
20	38.12921020247243	40.0	36.0	41.0	34.0	41.0
21	38.06972712246332	40.0	36.0	41.0	34.0	41.0
22	38.050631303550276	40.0	36.0	41.0	34.0	41.0
23	38.01448682461078	40.0	36.0	41.0	34.0	41.0
24	37.98575896642396	40.0	36.0	41.0	34.0	41.0
25	37.93701316054278	40.0	36.0	41.0	34.0	41.0
26	37.80754561980581	40.0	36.0	41.0	33.0	41.0
27	37.73654335324956	40.0	36.0	41.0	33.0	41.0
28	37.64560396960769	40.0	36.0	41.0	33.0	41.0
29	37.574101872960675	40.0	35.0	41.0	33.0	41.0
30	37.49188064783259	40.0	35.0	41.0	33.0	41.0
31	37.36182089916629	39.0	35.0	41.0	33.0	41.0
32	37.154782367709984	39.0	35.0	41.0	32.0	41.0
33	36.986385816042734	39.0	35.0	41.0	31.0	41.0
34	36.77294022164083	39.0	35.0	41.0	31.0	41.0
35	36.68991728884241	39.0	35.0	41.0	31.0	41.0
36	36.51389791588998	39.0	35.0	41.0	30.0	41.0
37	36.484851684080986	39.0	35.0	41.0	30.0	41.0
38	36.3487098445083	39.0	35.0	41.0	30.0	41.0
39	36.27687122859226	39.0	35.0	41.0	30.0	41.0
40	36.18954447827966	39.0	35.0	41.0	30.0	41.0
41	36.033356647740206	39.0	35.0	41.0	30.0	41.0
42	35.94985038089362	38.0	35.0	40.0	29.0	41.0
43	35.8552406277734	38.0	35.0	40.0	29.0	41.0
44	35.65686251868177	38.0	35.0	40.0	28.0	41.0
45	35.556596272554216	38.0	35.0	40.0	28.0	41.0
46	35.408054357759575	38.0	34.0	40.0	27.0	41.0
47	35.27203458890212	38.0	34.0	40.0	27.0	41.0
48	35.134609357215204	37.0	34.0	40.0	26.0	41.0
49	35.044009792050886	37.0	34.0	40.0	26.0	41.0
50	34.93886401652244	37.0	34.0	40.0	26.0	41.0
51	33.577234471450296	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	6.0
11	3.0
12	8.0
13	4.0
14	10.0
15	20.0
16	35.0
17	74.0
18	141.0
19	254.0
20	525.0
21	810.0
22	1262.0
23	2033.0
24	3180.0
25	4426.0
26	6023.0
27	7263.0
28	7851.0
29	8883.0
30	10708.0
31	13071.0
32	16131.0
33	21998.0
34	38383.0
35	50382.0
36	47043.0
37	68924.0
38	116702.0
39	179938.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4891868440761	21.043011781473624	24.654655348181972	11.813146026268297
2	33.70735360586995	23.496468197279473	24.91265345443628	17.883524742414295
3	31.39295882917688	24.249512541941186	27.150341633042235	17.2071869958397
4	27.88392064743668	25.89086878057954	26.974823739784824	19.250386832198956
5	24.977482901851847	29.80323520387459	25.669491888895852	19.54979000537771
6	26.15892947281947	34.12420860235629	24.55237988406581	15.164482040758424
7	80.564692530262	5.620201713608905	8.953392081239711	4.861713674889394
8	80.79101823472548	4.774614569964665	9.225081902851507	5.209285292458339
9	74.44977449909767	6.582910759708746	11.955506873900951	7.011807867292637
10	39.985087577490155	25.33198285731253	18.466000006598417	16.216929558598892
11	30.828959132704064	24.501242152007734	24.76220954592993	19.90758916935827
12	27.666832726828833	22.366489279221916	28.361481080688744	21.605196913260507
13	24.789592976644904	23.303959380144704	31.057099401853495	20.8493482413569
14	21.486260446118976	25.846824346839192	30.403031312788066	22.26388389425377
15	21.11691405231885	23.846184300386337	33.91751318858606	21.11938845870876
16	24.430474129256393	23.1789193772414	31.279960937371126	21.110645556131082
17	22.8194706090009	24.287453439919762	29.18908753789966	23.703988413179676
18	24.558978301105565	23.653510522825574	29.395453030817908	22.392058145250953
19	24.62611719448504	26.942986377568023	26.58436241145749	21.846534016489443
20	26.442166524250833	25.93870730411774	26.44018699913891	21.17893917249252
21	24.523016928238917	27.517873462156427	27.288908390877033	20.670201218727627
22	23.2721220179279	24.829513399735404	26.792872389913658	25.10549219242304
23	22.98146174732682	26.513429428280155	26.917252551112984	23.58785627328004
24	24.16472288298037	24.00207190295048	28.63053153548464	23.202673678584507
25	23.081757686331052	25.714361124766167	26.62197338858408	24.581907800318703
26	22.409543950406295	25.911653794254757	27.74865309812176	23.930149157217183
27	22.78779820721009	25.25593610092939	27.79533689867801	24.160928793182514
28	21.753661296654933	26.511284942742236	27.571815521456404	24.163238239146427
29	24.085871799355335	24.6165494897774	26.86776442331485	24.429814287552414
30	25.931449045374016	23.87505237493525	27.762674734331235	22.430823845359498
31	26.63863439160945	25.003546649158864	25.8227401246441	22.535078834587583
32	26.698185105393218	24.393687954259775	25.902580970825102	23.00554596952191
33	24.67181123248533	25.894497909951404	25.484901172208787	23.948789685354484
34	24.78365440130913	25.362500536121384	27.303094987512495	22.550750075056992
35	23.431968670715893	25.122813037152387	27.572145442308386	23.873072849823327
36	23.80082018323804	29.152136402477048	25.839731048521458	21.207312365763453
37	25.222614094878637	26.872053394390687	26.669976872548272	21.235355638182398
38	23.160608769956088	27.586661959795844	26.62032378432414	22.632405485923925
39	24.63156088854284	26.89135376423196	24.94647034176501	23.53061500546019
40	24.917767227642088	24.23400626189777	28.08451252544514	22.763713985014995
41	21.471249047353542	26.366944569997656	27.049385852334023	25.11242053031478
42	23.419431678340366	25.65200608374051	27.27158754614768	23.656974691771442
43	23.37406756119207	24.450599301227633	27.698670089045642	24.476663048534654
44	23.97287390754958	24.82291498269565	26.04081780780791	25.16339330194686
45	24.1586193472186	23.144442648208692	26.907519885979355	25.78941811859335
46	24.46132172891723	24.34288014305368	27.184653401648944	24.01114472638014
47	22.78829308848807	24.889394034371154	29.18743793363972	23.134874943501053
48	22.967440111117345	23.16885679125578	29.624583062523303	24.23912003510358
49	23.930149157217183	23.228572465465536	28.42466092384437	24.41661745347291
50	21.975862990468585	23.20366344114047	28.548546203765717	26.271927364625224
51	21.645447257202996	22.535738676291558	27.536513990293727	28.28230007621172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	469.0
2	677.0
3	2701.0
4	4725.0
5	3187.5
6	1650.0
7	1643.5
8	1637.0
9	1644.0
10	1651.0
11	1737.0
12	1823.0
13	1846.5
14	1870.0
15	1752.0
16	1634.0
17	1576.5
18	1519.0
19	1508.5
20	1498.0
21	1627.0
22	1756.0
23	1777.0
24	1798.0
25	2181.5
26	3015.5
27	3466.0
28	4103.5
29	4741.0
30	5634.5
31	6528.0
32	7653.0
33	8778.0
34	10166.5
35	11555.0
36	13162.5
37	14770.0
38	16175.0
39	17580.0
40	19514.0
41	21448.0
42	24138.5
43	26829.0
44	30897.0
45	34965.0
46	41259.0
47	47553.0
48	54233.5
49	60914.0
50	61874.5
51	62835.0
52	56528.0
53	50221.0
54	45231.5
55	40242.0
56	37296.0
57	34350.0
58	32504.5
59	30659.0
60	28397.5
61	26136.0
62	24543.5
63	22951.0
64	20118.0
65	17285.0
66	14770.0
67	12255.0
68	10362.5
69	8470.0
70	7263.0
71	6056.0
72	4863.5
73	3671.0
74	3377.5
75	2455.5
76	1827.0
77	1331.0
78	835.0
79	663.0
80	491.0
81	399.0
82	307.0
83	236.0
84	165.0
85	124.5
86	84.0
87	65.0
88	46.0
89	36.5
90	27.0
91	21.0
92	15.0
93	9.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	606206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.31954914154173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56730557940658	27.082509193436156
2	9.278591258448857	6.739885023510256
3	3.369377586493404	3.6712282448716937
4	1.7964594402654126	2.609863876860248
5	1.2007994279235699	2.1806246915802645
6	0.945153238297414	2.0596523690778135
7	0.8016782464448601	2.038161472722137
8	0.6901572591132291	2.005296039020374
9	0.6544032412172465	2.1390867609996573
>10	6.588518094162031	44.49086998134754
>50	0.07474274165059354	1.868454926397565
>100	0.030535144453436136	1.9103329469175836
>500	4.5574842467815133E-4	0.12116486588195749
>1k	0.0018229936987126053	1.0828696073767292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.46271399491262044	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1340	0.22104697083169747	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1294	0.21345879123598246	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	1103	0.1819513498711659	TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	732	0.12075103182746459	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2992085198760816E-4	0.0	0.0	0.20735525547421174	0.0
2	3.2992085198760816E-4	0.0	0.0	0.8437725789583079	0.0
3	3.2992085198760816E-4	0.0	0.0	1.2518846728669792	0.0
4	3.2992085198760816E-4	0.0	0.0	1.6852357119527026	0.0
5	3.2992085198760816E-4	0.0	0.0	2.8724558978301107	0.0
6	3.2992085198760816E-4	0.0	0.0	3.925398296948562	0.0
7	3.2992085198760816E-4	0.0	0.0	4.88150892600865	0.0
8	3.2992085198760816E-4	0.0	0.0	6.655988228424001	0.0
9	3.2992085198760816E-4	0.0	0.0	7.514277324869764	0.0
10	3.2992085198760816E-4	0.0	0.0	8.966094034041234	0.0
11	3.2992085198760816E-4	0.0	0.0	10.615863254405268	0.0
12	3.2992085198760816E-4	0.0	0.0	11.922844709554179	0.0
13	3.2992085198760816E-4	0.0	0.0	12.518846728669793	0.0
14	3.2992085198760816E-4	0.0	0.0	12.810991643104819	0.0
15	3.2992085198760816E-4	0.0	0.0	13.192710068854481	0.0
16	3.2992085198760816E-4	0.0	0.0	13.911442644909487	0.0
17	3.2992085198760816E-4	0.0	0.0	14.785732902676648	0.0
18	3.2992085198760816E-4	0.0	0.0	15.672230231967351	0.0
19	3.2992085198760816E-4	0.0	0.0	16.22418781734262	0.0
20	3.2992085198760816E-4	0.0	0.0	16.779114690385775	0.0
21	3.2992085198760816E-4	0.0	0.0	17.4627106957041	0.0
22	3.2992085198760816E-4	0.0	0.0	18.20717709821414	0.0
23	3.2992085198760816E-4	0.0	0.0	18.926074634695137	0.0
24	3.2992085198760816E-4	0.0	0.0	19.497992431615657	0.0
25	3.2992085198760816E-4	0.0	0.0	20.018112654774118	0.0
26	3.2992085198760816E-4	0.0	0.0	20.510189605513638	0.0
27	4.948812779814123E-4	0.0	0.0	20.997647664325328	0.0
28	4.948812779814123E-4	0.0	0.0	21.53459385093516	0.0
29	6.598417039752163E-4	0.0	0.0	22.066921145617165	0.0
30	6.598417039752163E-4	0.0	0.0	22.71966955127465	0.0
31	9.897625559628245E-4	0.0	0.0	23.258925183848394	0.0
32	9.897625559628245E-4	0.0	0.0	23.787623349158537	0.0
33	9.897625559628245E-4	0.0	0.0	24.313517187226783	0.0
34	9.897625559628245E-4	0.0	0.0	24.795861472832666	0.0
35	9.897625559628245E-4	0.0	0.0	25.318786023233027	0.0
36	0.0011547229819566286	0.0	0.0	25.833462552333696	0.0
37	0.0011547229819566286	0.0	0.0	26.346489477174426	0.0
38	0.0011547229819566286	0.0	0.0	26.900261627235626	0.0
39	0.0011547229819566286	0.0	0.0	27.45254913346288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTA	20	7.030645E-4	45.000004	14
AATGCGA	20	7.030645E-4	45.000004	25
TCCGTTA	20	7.030645E-4	45.000004	33
CTATGCG	25	3.8886166E-5	45.0	1
ACGTAAG	30	2.1637989E-6	44.999996	1
TTGCGAG	85	0.0	42.35294	1
CGTAAGG	85	0.0	42.35294	2
TGATTCG	70	0.0	41.785713	15
CGTTTTT	1560	0.0	40.961536	1
CACGACC	420	0.0	40.714287	27
AACACGT	400	0.0	39.9375	41
ACGACCA	430	0.0	39.767445	28
TTTAGCG	40	3.4546247E-7	39.375004	1
ATTCGTT	40	3.4546247E-7	39.375004	41
ACACGAC	435	0.0	39.310345	26
ACATACG	75	0.0	39.000004	17
CGACCAA	445	0.0	38.932583	29
CGAAAGG	110	0.0	38.863636	2
AAACACG	430	0.0	38.720932	40
CTAAGCG	35	6.2434065E-6	38.571426	1
>>END_MODULE