##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313407
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.041734868717036	33.0	31.0	34.0	30.0	34.0
2	32.14235802008251	33.0	31.0	34.0	30.0	34.0
3	32.25662796300019	34.0	31.0	34.0	30.0	34.0
4	35.78397100256216	37.0	35.0	37.0	35.0	37.0
5	35.759025803507896	37.0	35.0	37.0	35.0	37.0
6	35.77774267964659	37.0	35.0	37.0	35.0	37.0
7	35.987667792997605	37.0	35.0	37.0	35.0	37.0
8	35.9630257141672	37.0	35.0	37.0	35.0	37.0
9	37.52901179616282	39.0	37.0	39.0	35.0	39.0
10	37.26938134757679	39.0	37.0	39.0	34.0	39.0
11	37.21350193199258	39.0	37.0	39.0	34.0	39.0
12	37.022935033359175	39.0	37.0	39.0	33.0	39.0
13	36.89434186217921	39.0	35.0	39.0	33.0	39.0
14	37.965240087171	40.0	37.0	41.0	33.0	41.0
15	38.04875130421465	40.0	37.0	41.0	33.0	41.0
16	38.08698593203087	40.0	37.0	41.0	33.0	41.0
17	38.075543941264876	40.0	37.0	41.0	33.0	41.0
18	38.04099461722297	40.0	36.0	41.0	33.0	41.0
19	38.005449782551125	40.0	36.0	41.0	34.0	41.0
20	37.91272690144125	40.0	36.0	41.0	33.0	41.0
21	37.84357720153028	40.0	36.0	41.0	33.0	41.0
22	37.81729827349102	40.0	36.0	41.0	33.0	41.0
23	37.79114378428051	40.0	36.0	41.0	33.0	41.0
24	37.764389436100664	40.0	36.0	41.0	33.0	41.0
25	37.724690258992304	39.0	35.0	41.0	33.0	41.0
26	37.60891747791211	39.0	35.0	41.0	33.0	41.0
27	37.5473234484233	39.0	35.0	41.0	33.0	41.0
28	37.460714023617854	39.0	35.0	41.0	33.0	41.0
29	37.407894526925055	39.0	35.0	41.0	33.0	41.0
30	37.36715835957716	39.0	35.0	41.0	33.0	41.0
31	37.248025730120894	39.0	35.0	41.0	32.0	41.0
32	37.124308008436316	39.0	35.0	41.0	32.0	41.0
33	37.02238941695622	39.0	35.0	41.0	32.0	41.0
34	36.7940154495592	39.0	35.0	41.0	31.0	41.0
35	36.79461849926773	39.0	35.0	41.0	31.0	41.0
36	36.63548357247924	39.0	35.0	41.0	31.0	41.0
37	36.62948817352516	39.0	35.0	41.0	31.0	41.0
38	36.533111896032956	39.0	35.0	41.0	31.0	41.0
39	36.44185356421522	39.0	35.0	41.0	30.0	41.0
40	36.34566873107493	39.0	35.0	41.0	30.0	41.0
41	36.18073942190187	38.0	35.0	40.0	30.0	41.0
42	36.1076108702103	38.0	35.0	40.0	30.0	41.0
43	36.0259662355978	38.0	35.0	40.0	30.0	41.0
44	35.85124454782439	38.0	35.0	40.0	29.0	41.0
45	35.77873499953734	38.0	35.0	40.0	29.0	41.0
46	35.67837348878615	38.0	35.0	40.0	28.0	41.0
47	35.55923128711228	38.0	34.0	40.0	28.0	41.0
48	35.45390179542895	38.0	34.0	40.0	28.0	41.0
49	35.35568765215837	37.0	34.0	40.0	28.0	41.0
50	35.25509321744568	37.0	34.0	40.0	27.0	41.0
51	33.89110007115348	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	2.0
13	4.0
14	7.0
15	9.0
16	17.0
17	41.0
18	89.0
19	167.0
20	300.0
21	447.0
22	670.0
23	1029.0
24	1509.0
25	1983.0
26	2725.0
27	3177.0
28	3858.0
29	4635.0
30	5629.0
31	6976.0
32	9074.0
33	12195.0
34	21774.0
35	28600.0
36	24384.0
37	34806.0
38	59672.0
39	89579.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56345901655037	20.229924666647523	24.178464424853306	11.028151891948808
2	32.486511150038126	24.246746243702276	24.54603758052628	18.720705025733313
3	32.59467720886898	24.58496459874859	26.35008152338652	16.470276668995908
4	28.66847262505305	26.07344443487223	24.972320337452576	20.28576260262215
5	24.867025943900423	30.24374056737724	24.95860015889881	19.930633329823518
6	26.646182121011975	33.903199354194385	23.91714288449205	15.533475640301589
7	78.95420332028321	6.113137230502191	9.444907101628235	5.487752347586365
8	78.06430615780758	5.460311990478834	9.777382126117159	6.697999725596429
9	71.13465876639641	7.465372502847735	13.258478591735349	8.141490139020508
10	39.60345493240419	25.159297654487617	19.24813421525365	15.989113197854547
11	31.788377413395363	23.691557623154555	24.8210792994413	19.69898566400878
12	28.16274046208285	22.43663989636479	28.76291850533013	20.637701136222226
13	25.009013838235905	24.990507550884313	30.514953399254008	19.485525211625777
14	20.51358138139863	27.32006623974576	29.84872705459674	22.31762532425887
15	19.800451170522678	24.44616744361166	35.77297252454476	19.980408861320903
16	22.652014792266925	24.290459370722413	31.77114742172319	21.28637841528747
17	22.27295497547917	23.02788386985613	29.247591789589894	25.451569365074807
18	24.28503511408488	23.572223977128782	29.966146257103382	22.176594651682954
19	24.01509857788754	26.590982332877054	26.509937557233886	22.883981532001517
20	27.03736674675422	26.03930352544774	26.682875621795297	20.240454106002737
21	24.481903722635423	27.96076667081463	27.42153174625966	20.135797860290296
22	22.697642362806192	25.179718385358335	26.535782544742144	25.586856707093332
23	21.42198483122585	27.483751160631382	27.573730006030495	23.52053400211227
24	23.864814761635827	23.031393682974535	29.180267192500487	23.92352436288915
25	21.283187676088918	25.53261414071798	27.32453327462373	25.859664908569368
26	22.09076376724196	25.689279435366792	27.983420919124335	24.236535878266917
27	22.264339979643083	25.029434569106623	27.465244873279794	25.2409805779705
28	19.20123034903496	26.4027287201626	29.576237927040555	24.81980300376188
29	23.38046055129592	24.091995392572596	28.275054481871813	24.25248957425967
30	24.00425006461247	23.42449275223591	28.003841649995053	24.567415533156566
31	24.675262518067562	24.224730143232282	27.06097821682349	24.039029121876666
32	26.11939107933135	23.91586658881263	27.070231360499285	22.894510971356734
33	23.4685249531759	22.43791619204421	27.250827199137223	26.842731655642666
34	21.748078377317672	22.30613866314409	32.747194542559676	23.19858841697856
35	23.003315178027293	22.038116570465878	29.62601345853794	25.332554792968885
36	22.643399796430838	24.80576375128826	28.93394212637242	23.616894325908483
37	21.845395922873454	23.87949216194916	31.795397039632174	22.479714875545216
38	22.078000810447758	25.0820817658827	28.645499302823485	24.194418120846056
39	23.295267814694633	21.344449868701084	30.742453104110627	24.617829212493657
40	25.13472896265878	20.16802432619565	30.18981707492175	24.507429636223822
41	22.026948983270955	23.129030302450168	28.270268373073993	26.573752341204887
42	24.07859428793869	21.630978248730884	29.718544895295896	24.571882568034535
43	23.332599463317667	21.18906086973169	30.216300210269715	25.26203945668093
44	21.41049817011107	22.122352085307604	29.085821312223402	27.381328432357925
45	22.89163930607804	20.431260310076034	29.343952113386106	27.333148270459816
46	24.05466374394956	22.489925240980575	28.431719776520627	25.023691238549233
47	20.955817834317678	22.044817122782835	32.08352078926125	24.915844253638237
48	21.91463496348199	20.7251273902625	31.400064452931808	25.960173193323698
49	23.336747424275785	19.391079331348692	32.679869945470266	24.592303298905257
50	20.317989068527506	20.396162178891984	32.13074372939979	27.15510502318072
51	20.802662352787273	19.922656481827143	29.284604364293077	29.990076801092506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	321.0
2	476.0
3	850.0
4	1224.0
5	887.0
6	550.0
7	550.5
8	551.0
9	562.5
10	574.0
11	608.5
12	643.0
13	652.0
14	661.0
15	703.5
16	746.0
17	754.0
18	762.0
19	743.5
20	725.0
21	818.5
22	912.0
23	965.5
24	1019.0
25	1274.5
26	1647.5
27	1765.0
28	2041.5
29	2318.0
30	2775.0
31	3232.0
32	3746.5
33	4261.0
34	4865.5
35	5470.0
36	5774.5
37	6079.0
38	7125.5
39	8172.0
40	9379.5
41	10587.0
42	12760.0
43	14933.0
44	17481.0
45	20029.0
46	24133.5
47	28238.0
48	32118.5
49	35999.0
50	36560.5
51	37122.0
52	32508.0
53	27894.0
54	24621.0
55	21348.0
56	18933.0
57	16518.0
58	15043.5
59	13569.0
60	13277.5
61	12986.0
62	11544.0
63	10102.0
64	8745.5
65	7389.0
66	6048.0
67	4707.0
68	3983.0
69	3259.0
70	2828.5
71	2398.0
72	1933.5
73	1469.0
74	1359.0
75	1076.5
76	904.0
77	688.5
78	473.0
79	332.5
80	192.0
81	160.5
82	129.0
83	85.5
84	42.0
85	26.5
86	11.0
87	10.5
88	10.0
89	10.0
90	10.0
91	5.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	313407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.88739458455886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.89862334197709	28.348375305705957
2	10.467059009926782	8.140733077177696
3	3.7717109995910008	4.400160417000484
4	1.9880561853701213	3.092413013470432
5	1.3230577035559734	2.5725133488160723
6	0.9968764100715786	2.3259555786295194
7	0.8775960649641241	2.388919712488123
8	0.7773222256912509	2.4182428887842553
9	0.7773165421938478	2.7205033584057356
>10	6.005702129827961	38.069708146281286
>50	0.08381195392032445	2.2402403112520277
>100	0.02875900379618977	1.8216531185919
>500	0.002465057468244837	0.6186143549542042
>1k	0.0016433716454965584	0.8419673684423182
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1596	0.5092419760886004	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1039	0.3315178027293583	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	834	0.2661076491590807	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	567	0.18091491255779224	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	535	0.17070454712243185	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	387	0.12348160698388995	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	314	0.10018921083447402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.190739198550128E-4	0.0	0.0	0.30248207602255217	0.0
2	3.190739198550128E-4	0.0	0.0	1.545594067777682	0.0
3	3.190739198550128E-4	0.0	0.0	2.12152249311598	0.0
4	3.190739198550128E-4	0.0	0.0	2.817741786239618	0.0
5	3.190739198550128E-4	0.0	0.0	4.86045302115141	0.0
6	3.190739198550128E-4	0.0	0.0	6.026987272141337	0.0
7	3.190739198550128E-4	0.0	0.0	7.302325729801823	0.0
8	3.190739198550128E-4	0.0	0.0	9.446183397307655	0.0
9	3.190739198550128E-4	0.0	0.0	10.276094662850543	0.0
10	3.190739198550128E-4	0.0	0.0	12.060356022679775	0.0
11	3.190739198550128E-4	0.0	0.0	14.276324396072837	0.0
12	3.190739198550128E-4	0.0	0.0	15.958482101548466	0.0
13	3.190739198550128E-4	0.0	0.0	16.621517707007182	0.0
14	3.190739198550128E-4	0.0	0.0	16.896559425922202	0.0
15	3.190739198550128E-4	0.0	0.0	17.453343416069202	0.0
16	3.190739198550128E-4	0.0	0.0	18.542661778454214	0.0
17	3.190739198550128E-4	0.0	0.0	19.79821765308369	0.0
18	3.190739198550128E-4	0.0	0.0	21.111206833287067	0.0
19	3.190739198550128E-4	0.0	0.0	21.89804311964953	0.0
20	3.190739198550128E-4	0.0	0.0	22.615640365403454	0.0
21	3.190739198550128E-4	0.0	0.0	23.539997511223426	0.0
22	6.381478397100256E-4	0.0	0.0	24.43850966953514	0.0
23	6.381478397100256E-4	0.0	0.0	25.345317749763087	0.0
24	6.381478397100256E-4	0.0	0.0	26.042175190726436	0.0
25	6.381478397100256E-4	0.0	0.0	26.6573497082069	0.0
26	6.381478397100256E-4	0.0	0.0	27.224663137709115	0.0
27	6.381478397100256E-4	0.0	0.0	27.78910490193263	0.0
28	6.381478397100256E-4	0.0	0.0	28.411299045649905	0.0
29	6.381478397100256E-4	0.0	0.0	29.02519726745095	0.0
30	6.381478397100256E-4	0.0	0.0	29.716630451776762	0.0
31	6.381478397100256E-4	0.0	0.0	30.353821069727225	0.0
32	6.381478397100256E-4	0.0	0.0	30.937088195222188	0.0
33	6.381478397100256E-4	0.0	0.0	31.551943638782795	0.0
34	6.381478397100256E-4	0.0	0.0	32.1569077908279	0.0
35	6.381478397100256E-4	0.0	0.0	32.75963842543402	0.0
36	6.381478397100256E-4	0.0	0.0	33.3122744546229	0.0
37	6.381478397100256E-4	0.0	0.0	33.89266991483917	0.0
38	6.381478397100256E-4	0.0	0.0	34.51358776287702	0.0
39	6.381478397100256E-4	0.0	0.0	35.13961079363256	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.0254266E-4	45.0	5
AGCCGAT	20	7.0254266E-4	45.0	43
TGAACGG	55	1.8189894E-12	45.0	2
TAGCCGT	25	3.8842918E-5	45.0	44
GTATCGG	20	7.0254266E-4	45.0	2
GCTACGA	20	7.0254266E-4	45.0	10
GACTGGC	20	7.0254266E-4	45.0	9
CACCGGT	20	7.0254266E-4	45.0	39
CGGTAAT	20	7.0254266E-4	45.0	42
AGGGCGT	25	3.8842918E-5	45.0	6
TCGAACG	20	7.0254266E-4	45.0	1
ACGTTGG	30	2.160432E-6	44.999996	2
CAATTGG	30	2.160432E-6	44.999996	2
TGGTCAA	145	0.0	41.89655	14
GCGCAAG	70	0.0	41.785713	1
GGGAACA	400	0.0	41.625	7
ACGGGAG	195	0.0	41.53846	5
TACGGGA	60	3.6379788E-12	41.249996	4
TAAGGGA	215	0.0	40.813953	4
GTCAAGC	150	0.0	40.5	16
>>END_MODULE