##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481584
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.245066281271804	33.0	31.0	34.0	30.0	34.0
2	32.34916857702914	34.0	31.0	34.0	30.0	34.0
3	32.45347229143825	34.0	31.0	34.0	30.0	34.0
4	35.94841606033423	37.0	35.0	37.0	35.0	37.0
5	35.911818083657266	37.0	35.0	37.0	35.0	37.0
6	35.92749343832021	37.0	35.0	37.0	35.0	37.0
7	36.11380569121898	37.0	35.0	37.0	35.0	37.0
8	36.097380726934446	37.0	35.0	37.0	35.0	37.0
9	37.70581040898369	39.0	38.0	39.0	35.0	39.0
10	37.39404548323865	39.0	37.0	39.0	34.0	39.0
11	37.31933369879398	39.0	37.0	39.0	34.0	39.0
12	37.16351041562842	39.0	37.0	39.0	34.0	39.0
13	37.0594579554138	39.0	37.0	39.0	33.0	39.0
14	38.21978304927074	40.0	37.0	41.0	33.0	41.0
15	38.29659623243297	40.0	37.0	41.0	34.0	41.0
16	38.32387496262334	40.0	37.0	41.0	34.0	41.0
17	38.30623110402339	40.0	37.0	41.0	34.0	41.0
18	38.277868450779096	40.0	37.0	41.0	34.0	41.0
19	38.223576779959465	40.0	37.0	41.0	34.0	41.0
20	38.12796521479119	40.0	37.0	41.0	34.0	41.0
21	38.067259294328714	40.0	36.0	41.0	34.0	41.0
22	38.038537409880725	40.0	36.0	41.0	34.0	41.0
23	37.98334662281139	40.0	36.0	41.0	34.0	41.0
24	37.95666591913353	40.0	36.0	41.0	34.0	41.0
25	37.92881615668295	40.0	36.0	41.0	34.0	41.0
26	37.82210372437623	40.0	36.0	41.0	33.0	41.0
27	37.78016711518655	40.0	36.0	41.0	33.0	41.0
28	37.674328881358186	40.0	36.0	41.0	33.0	41.0
29	37.62492109372404	40.0	36.0	41.0	33.0	41.0
30	37.59112636632446	40.0	36.0	41.0	33.0	41.0
31	37.485933918070366	40.0	35.0	41.0	33.0	41.0
32	37.32939217249743	39.0	35.0	41.0	33.0	41.0
33	37.26365701518323	39.0	35.0	41.0	32.0	41.0
34	37.065083142297084	39.0	35.0	41.0	32.0	41.0
35	37.02414116748065	39.0	35.0	41.0	32.0	41.0
36	36.909988703943654	39.0	35.0	41.0	31.0	41.0
37	36.88489235522775	39.0	35.0	41.0	31.0	41.0
38	36.743812086780295	39.0	35.0	41.0	31.0	41.0
39	36.66207764377554	39.0	35.0	41.0	31.0	41.0
40	36.55173759925579	39.0	35.0	41.0	31.0	41.0
41	36.42587170670122	39.0	35.0	41.0	30.0	41.0
42	36.35178079005947	39.0	35.0	41.0	30.0	41.0
43	36.25610900694375	38.0	35.0	41.0	30.0	41.0
44	36.064815276255025	38.0	35.0	40.0	30.0	41.0
45	36.01403908767733	38.0	35.0	40.0	30.0	41.0
46	35.923722548921894	38.0	35.0	40.0	30.0	41.0
47	35.826526628791655	38.0	35.0	40.0	30.0	41.0
48	35.72670396026446	38.0	35.0	40.0	29.0	41.0
49	35.64210397355394	38.0	35.0	40.0	29.0	41.0
50	35.53513821057178	37.0	35.0	40.0	29.0	41.0
51	34.31977391275458	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	5.0
12	8.0
13	4.0
14	14.0
15	25.0
16	28.0
17	66.0
18	109.0
19	202.0
20	431.0
21	593.0
22	900.0
23	1431.0
24	2116.0
25	2837.0
26	3657.0
27	4361.0
28	5217.0
29	6181.0
30	7731.0
31	9689.0
32	12309.0
33	17006.0
34	31212.0
35	42032.0
36	37175.0
37	53187.0
38	91776.0
39	151197.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07870693378518	20.750897039768763	24.217581979467756	10.952814046978306
2	32.21930130569122	23.723379514269578	24.99127878002591	19.06604040001329
3	32.39517924183527	25.466169972424336	25.66405860659823	16.474592179142164
4	28.45256486926476	26.620070434233696	25.09780225256653	19.829562443935014
5	25.756046712515367	30.641383434665602	23.598583009402304	20.003986843416723
6	26.558398950131235	34.22393601116316	23.581140569454135	15.63652446925147
7	82.54447822186783	5.571198378683677	7.3393219043822056	4.5450014950662805
8	82.87567693278847	5.052701086414832	7.377736801887106	4.693885178909599
9	76.66305358982027	6.639132529319911	9.981643908435496	6.716169972424334
10	40.32712880826605	27.641076115485564	16.412713046945083	15.6190820293033
11	32.12274494169242	25.841597727499256	23.33300109638194	18.702656234426392
12	28.287484634041	24.018032160536894	26.828341473138646	20.866141732283463
13	25.249800657829162	26.01332270175089	28.363899132861558	20.37297750755839
14	21.27167846107844	27.473504103126352	28.838582677165352	22.416234758629855
15	21.97103724376225	25.02990132562544	32.21743247283963	20.781628957772682
16	26.05339878401276	24.71303033323366	28.890079404631386	20.343491478122196
17	24.02862221336257	24.555840725605503	27.63069371075451	23.78484335027742
18	26.642081132263527	23.264061928967738	27.836472972524007	22.257383966244724
19	25.06167148410246	27.560093358583345	25.383110734575897	21.995124422738296
20	27.654573241635934	26.600759161433935	25.244401807369016	20.500265789561116
21	26.057551745905176	28.140054486860027	25.324761619987374	20.47763214724742
22	25.2531230273431	24.21986610850859	25.679216917505563	24.847793946642746
23	23.50181899730888	28.351647895278916	25.89890860161467	22.247624505797532
24	24.253089803647963	24.40757998604605	28.4835044353633	22.85582577494269
25	23.50534901491744	26.330816638426523	26.309844180869796	23.853990165786236
26	22.39027874680222	26.59826738429848	26.83934682215356	24.17210704674574
27	23.494758962091762	25.694375228412902	26.556945413468885	24.253920396026444
28	20.513762915711485	27.89669091996412	27.82380643875212	23.765739725572278
29	22.734351639589356	25.35819296322137	27.261080102328982	24.646375294860295
30	24.12974683544304	23.569096979966112	28.49284859962125	23.8083075849696
31	24.277176982624006	25.706211169806302	26.17736469650154	23.83924715106814
32	24.319121897737467	24.890154157945446	27.448171035582575	23.34255290873451
33	23.730231901392074	24.313930695371937	26.73759925578923	25.21823814744676
34	21.634024386192234	25.060633243629354	30.300840559487025	23.004501810691387
35	21.88154091498056	25.240456493571216	29.81888933187149	23.05911325957673
36	23.177265025416126	27.304270906010164	27.041596066314494	22.476868002259213
37	23.060566796239076	25.9543506428785	28.567809561779463	22.41727299910296
38	23.400902023323034	26.256063324362938	27.916625137047742	22.426409515266286
39	25.675479251802386	23.10292700754178	27.196916841091067	24.02467689956477
40	26.077901259178045	22.57653908767733	28.045989899996677	23.299569753147946
41	22.622014020399348	25.03986843416725	26.83415561978803	25.50396192564537
42	25.745871955878936	24.128500946875313	26.688801953553277	23.43682514369248
43	24.4129788365062	22.98082992790458	28.30015116781288	24.306040067776337
44	23.274444333698792	24.491885112462207	27.69984052626333	24.533830027575668
45	23.36227947772351	23.05122263198113	27.69319578723546	25.893302103059902
46	23.508256088242135	24.236477956078275	27.24965945712482	25.005606498554766
47	21.97477490946543	24.446825475929433	29.705721120303004	23.872678494302136
48	23.434125718462408	23.574911126615504	28.775250008305925	24.215713146616167
49	24.73358749460115	22.786263663244625	28.574039004618097	23.90610983753613
50	21.902513372537292	23.557883982856573	29.085891557859068	25.453711086747067
51	22.107877338117547	22.378027509219574	27.33790989733878	28.1761852553241
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	452.0
2	668.0
3	1194.5
4	1721.0
5	1274.5
6	828.0
7	821.0
8	814.0
9	859.5
10	905.0
11	962.0
12	1019.0
13	1020.0
14	1021.0
15	1058.0
16	1095.0
17	1135.5
18	1176.0
19	1092.0
20	1008.0
21	1126.0
22	1244.0
23	1350.5
24	1457.0
25	1873.0
26	2469.0
27	2649.0
28	3182.0
29	3715.0
30	4691.5
31	5668.0
32	6415.5
33	7163.0
34	8263.5
35	9364.0
36	10463.0
37	11562.0
38	12853.5
39	14145.0
40	16337.5
41	18530.0
42	20642.5
43	22755.0
44	26397.5
45	30040.0
46	35388.0
47	40736.0
48	43875.5
49	47015.0
50	47652.0
51	48289.0
52	44385.0
53	40481.0
54	37318.5
55	34156.0
56	30818.0
57	27480.0
58	25124.0
59	22768.0
60	21557.5
61	20347.0
62	18555.5
63	16764.0
64	14921.0
65	13078.0
66	10956.0
67	8834.0
68	7372.5
69	5911.0
70	5175.0
71	4439.0
72	3951.5
73	3464.0
74	2976.5
75	2234.0
76	1979.0
77	1533.0
78	1087.0
79	904.0
80	721.0
81	499.5
82	278.0
83	191.0
84	104.0
85	69.0
86	34.0
87	32.0
88	30.0
89	21.5
90	13.0
91	11.0
92	9.0
93	6.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	481584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.877168466816194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16183518863667	24.05353980838249
2	9.260926633868259	6.089460902010235
3	3.3651702832867634	3.3191181096942746
4	1.7685980370955632	2.3258598246268503
5	1.255565643022689	2.0639721583401673
6	0.9588060222487544	1.8913696272282132
7	0.8726191586947672	2.008247296144549
8	0.7267968630592733	1.9116018326362612
9	0.6504153402611651	1.9245433243651113
>10	7.863265270903463	50.22481500934658
>50	0.0849410322165972	1.8850189225029714
>100	0.02852497350557369	1.6172191087477121
>500	0.001901664900371579	0.4689101797271073
>1k	6.338883001238597E-4	0.21632389624743883
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1038	0.21553872221668496	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.1777467689956477	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	728	0.151167812884149	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	666	0.13829363101764178	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15303664573573872	0.0
2	0.0	0.0	0.0	0.6779710289378384	0.0
3	0.0	0.0	0.0	0.9931808365726436	0.0
4	0.0	0.0	0.0	1.3470131898069704	0.0
5	0.0	0.0	0.0	2.3914831057510217	0.0
6	0.0	0.0	0.0	3.2422173494136017	0.0
7	0.0	0.0	0.0	4.058274361274461	0.0
8	0.0	0.0	0.0	5.551679457789295	0.0
9	0.0	0.0	0.0	6.248961759526894	0.0
10	0.0	0.0	0.0	7.455397189275391	0.0
11	0.0	0.0	0.0	8.806978637164026	0.0
12	0.0	0.0	0.0	9.894639356789263	0.0
13	0.0	0.0	0.0	10.390295358649789	0.0
14	0.0	0.0	0.0	10.626806538423203	0.0
15	0.0	0.0	0.0	10.96734941360178	0.0
16	0.0	0.0	0.0	11.56122296421808	0.0
17	0.0	0.0	0.0	12.290275424432705	0.0
18	0.0	0.0	0.0	13.071447556397223	0.0
19	2.0764809462108376E-4	0.0	0.0	13.546546396890262	0.0
20	2.0764809462108376E-4	0.0	0.0	14.01749227549088	0.0
21	2.0764809462108376E-4	0.0	0.0	14.557377321505697	0.0
22	2.0764809462108376E-4	0.0	0.0	15.127994285524435	0.0
23	2.0764809462108376E-4	0.0	0.0	15.708578358084987	0.0
24	2.0764809462108376E-4	0.0	0.0	16.187830160470448	0.0
25	2.0764809462108376E-4	0.0	0.0	16.610601681118975	0.0
26	2.0764809462108376E-4	0.0	0.0	17.046247383634007	0.0
27	2.0764809462108376E-4	0.0	0.0	17.42665869297983	0.0
28	2.0764809462108376E-4	0.0	0.0	17.836971327951094	0.0
29	2.0764809462108376E-4	0.0	0.0	18.29379713611748	0.0
30	2.0764809462108376E-4	0.0	0.0	18.821846240738896	0.0
31	2.0764809462108376E-4	0.0	0.0	19.29777567361042	0.0
32	2.0764809462108376E-4	0.0	0.0	19.76560683079172	0.0
33	2.0764809462108376E-4	0.0	0.0	20.198345459982058	0.0
34	2.0764809462108376E-4	0.0	0.0	20.612188112561878	0.0
35	2.0764809462108376E-4	0.0	0.0	21.075243363566894	0.0
36	4.152961892421675E-4	0.0	0.0	21.50715140037875	0.0
37	4.152961892421675E-4	0.0	0.0	21.932207050068108	0.0
38	4.152961892421675E-4	0.0	0.0	22.399207614870924	0.0
39	6.229442838632513E-4	0.0	0.0	22.877836472972525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCAG	25	3.8874176E-5	45.000004	1
TACGAAT	35	1.2099554E-7	45.000004	12
TATTACG	25	3.8874176E-5	45.000004	1
CGAATAT	35	1.2099554E-7	45.000004	14
GCGATTC	35	1.2099554E-7	45.000004	9
AGCTACG	35	1.2099554E-7	45.000004	9
CCGCTAT	20	7.0291996E-4	45.0	25
TCGATAG	20	7.0291996E-4	45.0	1
CCGTAGG	20	7.0291996E-4	45.0	2
ACGTACG	20	7.0291996E-4	45.0	1
GACCTAT	40	6.8012014E-9	45.0	33
TCGCGGT	20	7.0291996E-4	45.0	30
ACACAAC	300	0.0	40.5	11
CTCGTAG	40	3.452642E-7	39.375	1
GCGTTAG	40	3.452642E-7	39.375	1
GCTACGA	40	3.452642E-7	39.375	10
CTAGCGG	80	0.0	39.375	2
AACGAGC	300	0.0	39.0	15
GCGATTA	70	0.0	38.57143	9
ACAACGA	305	0.0	38.360657	13
>>END_MODULE