FastQCFastQC Report
Sat 18 Jun 2016
SRR3550443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765709
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.3390321910804235No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC18750.24487109332657708No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC16910.2208410767014623No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT13650.1782661559417481No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG11160.14574727474797866No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC7940.10369474565402784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG406.8102963E-945.01
TATTGCG351.2112105E-745.01
TCGTGCG207.03181E-445.01
TCTACGG207.03181E-445.02
TATAGCG253.889581E-545.01
TATCGTG207.03181E-445.01
TAGTACG253.889581E-545.01
CCCGACG302.1645483E-644.99999610
TGCGACG501.0804797E-940.51
CGTTTTT18850.040.2254681
CGAACGG403.4562072E-739.3752
CGCGCAA700.038.57142618
CGTCATA356.2455583E-638.57142638
ACGCGCA700.038.57142617
CATTCCG301.139566E-437.49999611
TAGCACG301.139566E-437.4999961
GTCGTAT301.139566E-437.49999634
TTTGCGC301.139566E-437.49999613
CTACGGG1700.037.0588233
ATAACGG552.746674E-936.818182