##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	765709
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13218337514643	33.0	31.0	34.0	30.0	34.0
2	32.25535288210012	34.0	31.0	34.0	30.0	34.0
3	32.33806576649876	34.0	31.0	34.0	30.0	34.0
4	35.85940089511812	37.0	35.0	37.0	35.0	37.0
5	35.8420640217106	37.0	35.0	37.0	35.0	37.0
6	35.86437145181786	37.0	35.0	37.0	35.0	37.0
7	36.08050577961079	37.0	35.0	37.0	35.0	37.0
8	36.08572055441427	37.0	35.0	37.0	35.0	37.0
9	37.69721395464857	39.0	38.0	39.0	35.0	39.0
10	37.256146917432076	39.0	37.0	39.0	34.0	39.0
11	37.262248452088194	39.0	37.0	39.0	34.0	39.0
12	37.20511969952032	39.0	37.0	39.0	34.0	39.0
13	37.167046488940315	39.0	37.0	39.0	34.0	39.0
14	38.34204769697104	40.0	38.0	41.0	34.0	41.0
15	38.40773191904496	40.0	38.0	41.0	34.0	41.0
16	38.423936508516945	40.0	38.0	41.0	34.0	41.0
17	38.45321003148716	40.0	38.0	41.0	34.0	41.0
18	38.38995884859653	40.0	38.0	41.0	34.0	41.0
19	38.303701536745685	40.0	37.0	41.0	34.0	41.0
20	38.18970130950531	40.0	37.0	41.0	34.0	41.0
21	38.15312997496438	40.0	37.0	41.0	34.0	41.0
22	38.13379234147698	40.0	37.0	41.0	34.0	41.0
23	38.11108789370375	40.0	37.0	41.0	34.0	41.0
24	38.09829190985087	40.0	37.0	41.0	34.0	41.0
25	38.046829800877354	40.0	37.0	41.0	34.0	41.0
26	37.93341073436515	40.0	36.0	41.0	34.0	41.0
27	37.855463367937425	40.0	36.0	41.0	33.0	41.0
28	37.78419869689399	40.0	36.0	41.0	33.0	41.0
29	37.72540220893316	40.0	36.0	41.0	33.0	41.0
30	37.66587567861942	40.0	36.0	41.0	33.0	41.0
31	37.56465445750278	40.0	36.0	41.0	33.0	41.0
32	37.37840223897068	40.0	36.0	41.0	32.0	41.0
33	37.23468967976085	40.0	36.0	41.0	32.0	41.0
34	37.06386629907706	40.0	35.0	41.0	31.0	41.0
35	36.992541552992066	40.0	35.0	41.0	31.0	41.0
36	36.83642088574119	39.0	35.0	41.0	31.0	41.0
37	36.794607350834326	39.0	35.0	41.0	31.0	41.0
38	36.739975630428795	39.0	35.0	41.0	31.0	41.0
39	36.66767009399132	39.0	35.0	41.0	30.0	41.0
40	36.623040868005994	39.0	35.0	41.0	30.0	41.0
41	36.46679090881784	39.0	35.0	41.0	30.0	41.0
42	36.388238874037	39.0	35.0	41.0	30.0	41.0
43	36.32516399833357	39.0	35.0	41.0	30.0	41.0
44	36.141924673733755	39.0	35.0	41.0	29.0	41.0
45	36.088332512743094	39.0	35.0	41.0	29.0	41.0
46	35.964700689165205	39.0	35.0	41.0	29.0	41.0
47	35.85348480950335	38.0	35.0	40.0	28.0	41.0
48	35.7228725272917	38.0	35.0	40.0	28.0	41.0
49	35.64820055660832	38.0	35.0	40.0	28.0	41.0
50	35.55275176339837	38.0	35.0	40.0	27.0	41.0
51	34.21747165045729	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	4.0
12	2.0
13	9.0
14	15.0
15	16.0
16	38.0
17	67.0
18	136.0
19	327.0
20	469.0
21	896.0
22	1410.0
23	2303.0
24	3578.0
25	5372.0
26	7288.0
27	8492.0
28	9295.0
29	10514.0
30	12538.0
31	15490.0
32	19382.0
33	25981.0
34	43572.0
35	57861.0
36	56665.0
37	82039.0
38	145838.0
39	255954.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11419481813587	21.92072967667874	25.578385522437376	12.386689982748015
2	33.02716828455719	24.32151117461072	25.074930554557934	17.57638998627416
3	31.18299510649607	23.38825846372447	28.241277038666123	17.187469391113332
4	27.602914423103293	25.685998205584625	27.728419020802942	18.982668350509137
5	24.234141168511798	30.453605743173974	26.542720537436548	18.76953255087768
6	25.40495148940394	34.537663786111956	25.424671774786507	14.632712949697602
7	80.46947339002153	5.476101234280908	9.278459571455997	4.775965804241559
8	81.90069595629672	4.369675686194102	8.89228153254043	4.837346824968755
9	75.80033668142858	6.31793540365857	11.452131292697358	6.429596622215489
10	38.559165427074774	22.121850467997632	22.123156447162042	17.19582765776555
11	29.678898902846907	25.572378018281093	23.904642625331554	20.844080453540446
12	24.429907445256617	23.140514216236195	30.708532876066496	21.721045462440692
13	23.573968700903347	24.479404055587697	32.5977623352997	19.348864908209254
14	21.575559383525594	27.115000607280308	31.07590481501458	20.23353519417951
15	21.15829904049711	25.72387160135247	33.54877636282191	19.569052995328512
16	22.764653412719454	25.16138637524177	31.167453954439612	20.906506257599165
17	22.73788083984908	25.552918928731412	29.15389527875472	22.555304952664784
18	23.487382282303066	25.027001119224145	30.620118086636044	20.86549851183674
19	23.243817168140897	27.6847993167117	28.036630103603326	21.03475341154407
20	24.10106189165858	27.079086180259083	27.910994907987234	20.908857020095102
21	23.0541889934688	27.009216294963228	29.722126813188822	20.214467898379148
22	22.29084417187208	25.48291844551912	27.81957636647865	24.406661016130148
23	21.333692042277157	27.207072138371103	28.454151642464698	23.005084176887042
24	22.19276513662501	25.52196722253493	29.285146184777766	23.00012145606229
25	21.22555696746414	27.005559553302888	28.278366846935327	23.49051663229765
26	21.222161421636677	27.171810700932074	29.402814907490963	22.203212969940278
27	22.163772399175144	26.248091637946008	29.52753591769197	22.060600045186877
28	20.707736228776206	26.05441492786424	30.237988583130143	22.999860260229408
29	22.376777600890154	24.515057286776045	29.119417428814344	23.988747683519456
30	22.85894510838974	24.712521336434598	29.851418750465253	22.577114804710405
31	22.703925381574464	25.38601479152002	28.51200651944799	23.398053307457534
32	22.35914688217064	25.256853452160023	28.707903394109252	23.676096271560084
33	21.059828211500715	24.58087863666223	30.58315887628329	23.77613427555377
34	21.661754008376548	24.30401105380765	30.753327961405702	23.280906976410098
35	21.79679225397638	23.730555602715913	29.54803979057318	24.924612352734524
36	22.166906749169723	24.762801534264323	30.98082953184565	22.089462184720304
37	21.811288622701312	25.22080842722235	30.69834623858411	22.269556711492225
38	20.086481940267127	25.603982714059782	29.17035061622627	25.13918472944683
39	21.942278332891476	23.69111503195078	30.554296736749865	23.81230989840788
40	23.102249026719026	23.14168959748416	30.238772170628792	23.51728920516802
41	20.824229570241435	24.05548321882073	29.48796474901039	25.632322461927444
42	21.569290683536437	23.679491817387547	30.88000794035332	23.8712095587227
43	21.60886185221801	22.955979360305285	31.567867166247233	23.867291621229477
44	21.3060052839917	24.023356131376282	29.65839502996569	25.01224355466633
45	22.41308382166071	23.069599547608817	29.13260781837487	25.384708812355605
46	23.28417192432112	23.585853111299464	29.000312129020294	24.129662835359124
47	21.344531669341748	23.544975963453478	31.225700625172227	23.884791742032547
48	20.73476999747946	22.73135094402704	31.449153660202505	25.084725398290995
49	21.691922127074385	22.18806361163314	31.78779405753361	24.33222020375887
50	20.61997442892796	21.543301698164708	31.768726761733245	26.06799711117409
51	20.84525583478841	21.8835092704931	29.19033209744172	28.080902797276774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	459.5
2	762.0
3	2753.5
4	4745.0
5	3384.5
6	2024.0
7	1913.5
8	1803.0
9	1878.5
10	1954.0
11	2032.5
12	2111.0
13	2079.0
14	2047.0
15	2019.0
16	1991.0
17	1972.0
18	1953.0
19	1992.5
20	2032.0
21	2174.0
22	2316.0
23	2523.0
24	2730.0
25	3528.0
26	4925.5
27	5525.0
28	6319.5
29	7114.0
30	8679.0
31	10244.0
32	11600.5
33	12957.0
34	14891.0
35	16825.0
36	19204.5
37	21584.0
38	24305.5
39	27027.0
40	30639.5
41	34252.0
42	38300.0
43	42348.0
44	48566.0
45	54784.0
46	63840.5
47	72897.0
48	80270.5
49	87644.0
50	85694.0
51	83744.0
52	73168.5
53	62593.0
54	54557.0
55	46521.0
56	41125.5
57	35730.0
58	32017.5
59	28305.0
60	26008.5
61	23712.0
62	21625.0
63	19538.0
64	16913.0
65	14288.0
66	12262.0
67	10236.0
68	8232.0
69	6228.0
70	5042.0
71	3856.0
72	3237.0
73	2618.0
74	2234.5
75	1436.0
76	1021.0
77	821.5
78	622.0
79	470.0
80	318.0
81	267.0
82	216.0
83	154.5
84	93.0
85	60.5
86	28.0
87	21.0
88	14.0
89	10.0
90	6.0
91	6.5
92	7.0
93	5.0
94	3.0
95	6.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	765709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.42127928338869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98289179148067	26.581302848468535
2	10.436619862753096	7.602300935917844
3	3.821867627220463	4.175919247054155
4	2.015910763722594	2.9368819574371994
5	1.2824026940944315	2.335337333769168
6	0.9939378704344572	2.172029326165798
7	0.8143117391121828	2.0760792691762733
8	0.6931340456801043	2.0195862926832135
9	0.5809741744122993	1.904384039643587
>10	6.265164424931142	42.6168241588019
>50	0.07494978268165875	1.8272377318086044
>100	0.034231868860085696	2.223841209409008
>500	0.001801677308425519	0.39397883214197393
>1k	0.001801677308425519	1.1342968175226786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2596	0.3390321910804235	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1875	0.24487109332657708	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1691	0.2208410767014623	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	1365	0.1782661559417481	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1116	0.14574727474797866	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	794	0.10369474565402784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.835874986450466E-4	0.0	0.0	0.20503872881212054	0.0
2	7.835874986450466E-4	0.0	0.0	0.9310325463067562	0.0
3	7.835874986450466E-4	0.0	0.0	1.4406256162589182	0.0
4	7.835874986450466E-4	0.0	0.0	1.9836517528199356	0.0
5	7.835874986450466E-4	0.0	0.0	3.46097538359873	0.0
6	7.835874986450466E-4	0.0	0.0	4.578501754583008	0.0
7	7.835874986450466E-4	0.0	0.0	5.646792711069088	0.0
8	7.835874986450466E-4	0.0	0.0	7.352793293535795	0.0
9	7.835874986450466E-4	0.0	0.0	8.065988515219228	0.0
10	7.835874986450466E-4	0.0	0.0	9.467565354462335	0.0
11	7.835874986450466E-4	0.0	0.0	11.241738049311161	0.0
12	7.835874986450466E-4	0.0	0.0	12.537008184571423	0.0
13	7.835874986450466E-4	0.0	0.0	13.109549450248071	0.0
14	7.835874986450466E-4	0.0	0.0	13.340968958181241	0.0
15	7.835874986450466E-4	0.0	0.0	13.728322378344776	0.0
16	7.835874986450466E-4	0.0	0.0	14.586742483110424	0.0
17	7.835874986450466E-4	0.0	0.0	15.6201637959068	0.0
18	7.835874986450466E-4	0.0	0.0	16.727895323158016	0.0
19	7.835874986450466E-4	0.0	0.0	17.351108580413708	0.0
20	7.835874986450466E-4	0.0	0.0	17.96753074601448	0.0
21	7.835874986450466E-4	0.0	0.0	18.756864552982922	0.0
22	7.835874986450466E-4	0.0	0.0	19.587859095295993	0.0
23	7.835874986450466E-4	0.0	0.0	20.482977214581517	0.0
24	9.141854150858877E-4	0.0	0.0	21.199176188343092	0.0
25	9.141854150858877E-4	0.0	0.0	21.851643378881533	0.0
26	9.141854150858877E-4	0.0	0.0	22.45056542367923	0.0
27	9.141854150858877E-4	0.0	0.0	23.034991099751995	0.0
28	0.001044783331526729	0.0	0.0	23.623334713317984	0.0
29	0.001044783331526729	0.0	0.0	24.266137658039803	0.0
30	0.00117538124796757	0.0	0.0	24.987038156793247	0.0
31	0.00117538124796757	0.0	0.0	25.62879631818354	0.0
32	0.00117538124796757	0.0	0.0	26.276823179562992	0.0
33	0.00117538124796757	0.0	0.0	26.887237841007483	0.0
34	0.00117538124796757	0.0	0.0	27.497391306619093	0.0
35	0.00117538124796757	0.0	0.0	28.08508193060288	0.0
36	0.00117538124796757	0.0	0.0	28.654488846284945	0.0
37	0.00117538124796757	0.0	0.0	29.261377363985535	0.0
38	0.00117538124796757	0.0	0.0	29.86121359419832	0.0
39	0.00117538124796757	0.0	0.0	30.489520170195206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8102963E-9	45.0	1
TATTGCG	35	1.2112105E-7	45.0	1
TCGTGCG	20	7.03181E-4	45.0	1
TCTACGG	20	7.03181E-4	45.0	2
TATAGCG	25	3.889581E-5	45.0	1
TATCGTG	20	7.03181E-4	45.0	1
TAGTACG	25	3.889581E-5	45.0	1
CCCGACG	30	2.1645483E-6	44.999996	10
TGCGACG	50	1.0804797E-9	40.5	1
CGTTTTT	1885	0.0	40.225468	1
CGAACGG	40	3.4562072E-7	39.375	2
CGCGCAA	70	0.0	38.571426	18
CGTCATA	35	6.2455583E-6	38.571426	38
ACGCGCA	70	0.0	38.571426	17
CATTCCG	30	1.139566E-4	37.499996	11
TAGCACG	30	1.139566E-4	37.499996	1
GTCGTAT	30	1.139566E-4	37.499996	34
TTTGCGC	30	1.139566E-4	37.499996	13
CTACGGG	170	0.0	37.058823	3
ATAACGG	55	2.746674E-9	36.81818	2
>>END_MODULE