FastQCFastQC Report
Sat 18 Jun 2016
SRR3550442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777244
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.3852072193545399No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC14600.1878431998188471TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC13980.17986629681284128TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT11200.14409889301171833TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG8310.10691623222565887No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.031876E-445.01
TGCGTTG302.164592E-644.9999961
CGTTTTT17900.041.3547481
GTTACGG1000.040.52
TTACGGG2000.040.53
TATAGCG451.9268555E-840.0000041
TATGCGG850.039.7058832
TCGTTAG403.4562981E-739.3751
CGTTCGG1150.039.13043245
TGTTGCG750.039.0000041
CAATACG356.2456784E-638.571431
TAGTACG356.2456784E-638.571431
TGCGTAG356.2456784E-638.571431
CACGAGG659.094947E-1238.076922
CGAATAT950.037.89473714
TAGGGCG2450.037.653065
TTAGGCG301.1395821E-437.4999961
CGTTGAT301.1395821E-437.49999625
GCGCATA301.1395821E-437.49999636
TAGTTCG301.1395821E-437.4999961
Produced by FastQC (version 0.11.5)