SRR3550438_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614680
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3911	0.636266024598165	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1345	0.21881304093186701	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	985	0.1602459816489881	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	882	0.1434892952430533	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	833	0.13551766772955035	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	775	0.1260818637339754	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	636	0.10346847139975272	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGCG	35	1.2107012E-7	45.000004	1
TCGTTAG	25	3.88868E-5	45.0	1
TAGCGCG	20	7.030722E-4	45.0	1
TAACGCC	20	7.030722E-4	45.0	12
TCGATCA	40	6.8066583E-9	45.0	17
CGGTCTA	20	7.030722E-4	45.0	31
TGTACGC	20	7.030722E-4	45.0	35
TACACCG	20	7.030722E-4	45.0	1
AATCGTT	20	7.030722E-4	45.0	22
CGTTTTT	2315	0.0	41.889847	1
TACGGTT	50	1.0804797E-9	40.5	33
CGAATAT	50	1.0804797E-9	40.5	14
CTCGATC	45	1.925764E-8	40.0	16
GCGTAAG	80	0.0	39.375	1
CACGACC	150	0.0	39.0	27
CGTAAGG	105	0.0	38.57143	2
TACGCGG	30	1.1393039E-4	37.500004	2
AACACGT	150	0.0	37.5	41
CGGGTAC	55	2.744855E-9	36.81818	6
TACGAAT	55	2.744855E-9	36.81818	12