##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614680
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37295503351337	33.0	31.0	34.0	31.0	34.0
2	32.49158911954188	34.0	31.0	34.0	31.0	34.0
3	32.557431509077894	34.0	31.0	34.0	31.0	34.0
4	36.05259321923602	37.0	35.0	37.0	35.0	37.0
5	36.02418006117004	37.0	35.0	37.0	35.0	37.0
6	36.04139877659921	37.0	35.0	37.0	35.0	37.0
7	36.19963070215397	37.0	36.0	37.0	35.0	37.0
8	36.199266284896204	37.0	37.0	37.0	35.0	37.0
9	37.80630734691221	39.0	38.0	39.0	35.0	39.0
10	37.53140495867768	39.0	37.0	39.0	35.0	39.0
11	37.49045356933689	39.0	37.0	39.0	35.0	39.0
12	37.399137762738334	39.0	37.0	39.0	35.0	39.0
13	37.359400013014906	39.0	37.0	39.0	35.0	39.0
14	38.61392269148175	40.0	38.0	41.0	35.0	41.0
15	38.683523133988416	40.0	38.0	41.0	35.0	41.0
16	38.685003579098066	40.0	38.0	41.0	35.0	41.0
17	38.69132882150062	40.0	38.0	41.0	35.0	41.0
18	38.606987375544996	40.0	38.0	41.0	35.0	41.0
19	38.523988091364615	40.0	37.0	41.0	35.0	41.0
20	38.386449860089805	40.0	37.0	41.0	34.0	41.0
21	38.35766252358951	40.0	37.0	41.0	34.0	41.0
22	38.33195158456433	40.0	37.0	41.0	34.0	41.0
23	38.299965835882084	40.0	37.0	41.0	34.0	41.0
24	38.28833702088892	40.0	37.0	41.0	34.0	41.0
25	38.23119672024468	40.0	37.0	41.0	34.0	41.0
26	38.11752781935316	40.0	37.0	41.0	34.0	41.0
27	38.07222131840958	40.0	36.0	41.0	34.0	41.0
28	37.977404503156116	40.0	36.0	41.0	34.0	41.0
29	37.91964436780113	40.0	36.0	41.0	34.0	41.0
30	37.87593219236025	40.0	36.0	41.0	33.0	41.0
31	37.75120713216633	40.0	36.0	41.0	33.0	41.0
32	37.55633988416737	40.0	36.0	41.0	33.0	41.0
33	37.38585117459491	40.0	36.0	41.0	33.0	41.0
34	37.13124877985293	40.0	35.0	41.0	32.0	41.0
35	37.07030162035531	40.0	35.0	41.0	32.0	41.0
36	36.918266415045224	40.0	35.0	41.0	31.0	41.0
37	36.863870631873496	39.0	35.0	41.0	31.0	41.0
38	36.7468731697794	39.0	35.0	41.0	31.0	41.0
39	36.682856120257696	39.0	35.0	41.0	31.0	41.0
40	36.58462940066376	39.0	35.0	41.0	30.0	41.0
41	36.496085768204594	39.0	35.0	41.0	30.0	41.0
42	36.42444198607406	39.0	35.0	41.0	30.0	41.0
43	36.3386851695191	39.0	35.0	41.0	30.0	41.0
44	36.17419470293486	39.0	35.0	41.0	30.0	41.0
45	36.13374113359797	39.0	35.0	41.0	30.0	41.0
46	36.065800091104315	39.0	35.0	41.0	30.0	41.0
47	35.988154812260035	39.0	35.0	41.0	29.0	41.0
48	35.85816197045617	38.0	35.0	40.0	29.0	41.0
49	35.76416997462094	38.0	35.0	40.0	29.0	41.0
50	35.68095757141928	38.0	35.0	40.0	28.0	41.0
51	34.45114693824429	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	2.0
11	4.0
12	1.0
13	7.0
14	11.0
15	17.0
16	39.0
17	68.0
18	114.0
19	230.0
20	442.0
21	618.0
22	1017.0
23	1587.0
24	2557.0
25	3872.0
26	5710.0
27	6983.0
28	7549.0
29	7979.0
30	8988.0
31	10792.0
32	13896.0
33	18779.0
34	33676.0
35	45027.0
36	42544.0
37	63732.0
38	117084.0
39	221193.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36230233617492	20.731762868484417	24.677230428841025	14.228704366499642
2	33.525411596277735	23.884297520661157	25.422821630767228	17.167469252293877
3	31.241947029348605	23.561039890674824	28.915207913060453	16.28180516691612
4	28.150907789418884	25.463655885989457	28.065660180907138	18.319776143684518
5	25.206123511420575	29.859276371445304	26.76384460206937	18.170755515064748
6	26.539662914036573	34.05739571809722	25.143814667794622	14.259126700071581
7	80.37482917941043	5.643261534456953	9.517635192295177	4.464274093837444
8	82.35553458710223	4.526745623739181	8.961736187935186	4.155983601223401
9	75.86256263421618	6.8743085833279105	11.51623609032342	5.746892692132492
10	37.8156113750244	28.25665386867964	18.865425912670005	15.062308843625951
11	28.913092991475235	24.72473482137047	27.737359276371443	18.624812910782847
12	26.239669421487605	22.943808160343593	30.818311967202444	19.998210450966354
13	23.540541419925816	23.487505694019653	32.53497754929394	20.43697533676059
14	20.76543892757207	26.408212403201663	31.40756165809852	21.41878701112774
15	20.707197240840763	25.80204333962387	33.662556126765146	19.828203292770223
16	23.724539597839524	24.648923016854297	31.47767944296219	20.148857942343984
17	23.523296674692524	24.555866467104835	29.5700201730982	22.350816685104444
18	23.42047894839591	25.322281512331617	30.018708921715366	21.238530617557103
19	24.1914492093447	26.005238498080303	28.745526127415893	21.057786165159108
20	25.127546040216046	25.912181948330836	28.508492223596015	20.451779787857095
21	24.67234984056745	26.002147458840376	28.937007874015748	20.38849482657643
22	23.17189431899525	24.679182664150453	28.855339363571293	23.293583653283008
23	22.486008980282424	26.417160148369884	29.061950933819226	22.03487993752847
24	23.163760005205962	25.2241816880328	30.00959849027136	21.60245981648988
25	22.60574607926075	26.468731697793974	28.4892952430533	22.436226979891977
26	22.162263291468733	27.022841153120325	28.741133597969675	22.07376195744127
27	22.902648532569792	26.01223400793909	28.91341836402681	22.171699095464305
28	21.82387583783432	26.385110952040087	30.147231079586128	21.643782130539467
29	22.9563350035791	24.766057135420056	29.845610724279297	22.431997136721545
30	23.97149736448233	24.77777054727663	29.591982820329278	21.65874926791176
31	24.123120973514673	25.972050497820003	27.963655885989457	21.941172642675863
32	24.55098587883126	25.917225222880198	28.63766512656992	20.89412377171862
33	24.633955879482006	25.76869265308779	27.97309168998503	21.62425977744517
34	22.801620355306827	26.401379579618663	29.73823778226069	21.058762282813824
35	24.65412897767944	24.817628684844145	28.562992125984255	21.965250211492158
36	24.380002602980415	27.064163467169912	28.202316652567188	20.35351727728249
37	25.1578056875122	27.024630702153967	27.86620680679378	19.951356803540055
38	24.3858593089087	26.106429361619053	28.167827162100608	21.33988416737164
39	24.92516431313854	25.211817531073077	27.892887356022644	21.970130799765734
40	24.44410099564001	25.202219040801715	29.376586191188913	20.97709377236936
41	23.193856966226328	26.109032342031625	28.146189887421098	22.55092080432095
42	24.314602720114532	25.613489945988157	27.853354591006703	22.21855274289061
43	24.329895230038396	25.105258020433396	28.574054792737684	21.990791956790527
44	23.60496518513698	25.297065139584824	28.345317888982883	22.75265178629531
45	23.130897377497234	24.432550270059217	28.553556321988676	23.88299603045487
46	23.51955489034945	25.610236220472444	28.39509988937333	22.475108999804778
47	23.138543632459164	25.175701177848637	29.733031821435546	21.952723368256656
48	23.383061104965186	24.09481356152795	29.578967918266414	22.94315741524045
49	23.27357324136136	24.327129563350034	29.70700201730982	22.692295177978785
50	22.174302075876877	24.343398190928614	29.790297390512137	23.692002342682372
51	22.592568490922105	24.089607600702806	28.509631027526517	24.808192880848573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	283.0
1	545.0
2	807.0
3	3821.0
4	6835.0
5	4577.0
6	2319.0
7	2275.0
8	2231.0
9	2237.0
10	2243.0
11	2222.5
12	2202.0
13	2143.5
14	2085.0
15	2015.5
16	1946.0
17	1884.0
18	1822.0
19	1912.5
20	2003.0
21	2009.5
22	2016.0
23	2147.5
24	2279.0
25	2885.5
26	4347.0
27	5202.0
28	6006.5
29	6811.0
30	7980.5
31	9150.0
32	10625.0
33	12100.0
34	14175.0
35	16250.0
36	17433.5
37	18617.0
38	19922.0
39	21227.0
40	23770.0
41	26313.0
42	29328.5
43	32344.0
44	36058.5
45	39773.0
46	44223.0
47	48673.0
48	52998.5
49	57324.0
50	57261.5
51	57199.0
52	51154.5
53	45110.0
54	40243.5
55	35377.0
56	33017.5
57	30658.0
58	29860.0
59	29062.0
60	27192.0
61	25322.0
62	23248.0
63	21174.0
64	18193.0
65	15212.0
66	12766.0
67	10320.0
68	8628.0
69	6936.0
70	5671.5
71	4407.0
72	3874.5
73	3342.0
74	2649.0
75	1576.0
76	1196.0
77	849.0
78	502.0
79	416.5
80	331.0
81	217.5
82	104.0
83	80.5
84	57.0
85	43.5
86	30.0
87	23.0
88	16.0
89	13.0
90	10.0
91	9.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	614680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.9463181900857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8101901085425	24.37691562715128
2	8.293572378835648	5.630724938089217
3	3.320050161975559	3.381104376164036
4	2.198063973700297	2.984647162135778
5	1.607743730938125	2.728849012927056
6	1.4530226975975828	2.9594862498038528
7	1.2125008749005868	2.8811958353592826
8	1.071336071057302	2.9094332125301947
9	1.0134991707831875	3.096410880311467
>10	7.932930605049251	44.64506445868613
>50	0.06255099149575247	1.4144949962368254
>100	0.02068994334005581	1.3679440638685287
>500	0.0028869688381473226	0.765231992743171
>1k	9.623229460491075E-4	0.8584971939931925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3911	0.636266024598165	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1345	0.21881304093186701	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	985	0.1602459816489881	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	882	0.1434892952430533	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	833	0.13551766772955035	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	775	0.1260818637339754	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	636	0.10346847139975272	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2537255157154944E-4	0.0	0.0	0.15227435413548512	0.0
2	3.2537255157154944E-4	0.0	0.0	0.6507451031430989	0.0
3	3.2537255157154944E-4	0.0	0.0	1.0387518708921715	0.0
4	3.2537255157154944E-4	0.0	0.0	1.3982885403787337	0.0
5	3.2537255157154944E-4	0.0	0.0	2.328854037873365	0.0
6	3.2537255157154944E-4	0.0	0.0	3.175798789614108	0.0
7	3.2537255157154944E-4	0.0	0.0	3.879904991214941	0.0
8	3.2537255157154944E-4	0.0	0.0	5.008134313789289	0.0
9	3.2537255157154944E-4	0.0	0.0	5.531821435543698	0.0
10	3.2537255157154944E-4	0.0	0.0	6.416834775818312	0.0
11	3.2537255157154944E-4	0.0	0.0	7.416379254246112	0.0
12	4.880588273573241E-4	0.0	0.0	8.166037613066962	0.0
13	4.880588273573241E-4	0.0	0.0	8.532081733584954	0.0
14	4.880588273573241E-4	0.0	0.0	8.713802303637666	0.0
15	4.880588273573241E-4	0.0	0.0	8.97426303117069	0.0
16	4.880588273573241E-4	0.0	0.0	9.45288605453244	0.0
17	4.880588273573241E-4	0.0	0.0	10.01041192165029	0.0
18	4.880588273573241E-4	0.0	0.0	10.613652632263943	0.0
19	4.880588273573241E-4	0.0	0.0	10.988319125398581	0.0
20	4.880588273573241E-4	0.0	0.0	11.382507971627513	0.0
21	4.880588273573241E-4	0.0	0.0	11.875284700982625	0.0
22	4.880588273573241E-4	0.0	0.0	12.382703195158456	0.0
23	4.880588273573241E-4	0.0	0.0	12.907041062016008	0.0
24	4.880588273573241E-4	0.0	0.0	13.317010476996161	0.0
25	4.880588273573241E-4	0.0	0.0	13.699648597644302	0.0
26	4.880588273573241E-4	0.0	0.0	14.060324071061364	0.0
27	4.880588273573241E-4	0.0	0.0	14.43092340730136	0.0
28	4.880588273573241E-4	0.0	0.0	14.804939155332857	0.0
29	4.880588273573241E-4	0.0	0.0	15.198802629010217	0.0
30	4.880588273573241E-4	0.0	0.0	15.674659985683608	0.0
31	4.880588273573241E-4	0.0	0.0	16.079260753562828	0.0
32	4.880588273573241E-4	0.0	0.0	16.47231079586126	0.0
33	4.880588273573241E-4	0.0	0.0	16.853159367475758	0.0
34	4.880588273573241E-4	0.0	0.0	17.240190017570118	0.0
35	4.880588273573241E-4	0.0	0.0	17.641699746209408	0.0
36	4.880588273573241E-4	0.0	0.0	18.073469122144857	0.0
37	6.507451031430989E-4	0.0	0.0	18.480835556712435	0.0
38	6.507451031430989E-4	0.0	0.0	18.890154226589445	0.0
39	6.507451031430989E-4	0.0	0.0	19.294429621917097	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGCG	35	1.2107012E-7	45.000004	1
TCGTTAG	25	3.88868E-5	45.0	1
TAGCGCG	20	7.030722E-4	45.0	1
TAACGCC	20	7.030722E-4	45.0	12
TCGATCA	40	6.8066583E-9	45.0	17
CGGTCTA	20	7.030722E-4	45.0	31
TGTACGC	20	7.030722E-4	45.0	35
TACACCG	20	7.030722E-4	45.0	1
AATCGTT	20	7.030722E-4	45.0	22
CGTTTTT	2315	0.0	41.889847	1
TACGGTT	50	1.0804797E-9	40.5	33
CGAATAT	50	1.0804797E-9	40.5	14
CTCGATC	45	1.925764E-8	40.0	16
GCGTAAG	80	0.0	39.375	1
CACGACC	150	0.0	39.0	27
CGTAAGG	105	0.0	38.57143	2
TACGCGG	30	1.1393039E-4	37.500004	2
AACACGT	150	0.0	37.5	41
CGGGTAC	55	2.744855E-9	36.81818	6
TACGAAT	55	2.744855E-9	36.81818	12
>>END_MODULE