FastQCFastQC Report
Sat 18 Jun 2016
SRR3550436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559200
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.2505364806866953No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC9070.16219599427753933TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC8680.15522174535050073TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT6900.12339055793991417TruSeq Adapter, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG253.8882266E-545.0000042
CGGACAT207.030177E-445.036
GATCGCG207.030177E-445.01
GTTAGCG351.2104283E-745.01
CCATCGC207.030177E-445.024
GCTACGA551.8189894E-1245.010
TGTAACG406.8048394E-945.01
CGTAAGG750.042.02
TTAACGG700.041.7857132
CTACGAA603.6379788E-1241.25000411
CGAATAT603.6379788E-1241.25000414
CGTTCAT451.9252184E-840.017
CGTTAGG800.039.3752
GCGTACG403.4539698E-739.3751
CGTTTTT8450.038.6094671
TCCGGCA356.242537E-638.57142624
GCGTTAG700.038.5714261
TACGAAT659.094947E-1238.0769212
ACGGGCT1250.037.85
TAAGGCG301.1391724E-437.5000041
Produced by FastQC (version 0.11.5)