##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559200
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.294443848354796	33.0	31.0	34.0	30.0	34.0
2	32.38746065808298	34.0	31.0	34.0	30.0	34.0
3	32.4876895565093	34.0	31.0	34.0	30.0	34.0
4	35.98801144492132	37.0	35.0	37.0	35.0	37.0
5	35.955779685264666	37.0	35.0	37.0	35.0	37.0
6	35.97437947067239	37.0	35.0	37.0	35.0	37.0
7	36.16379291845494	37.0	35.0	37.0	35.0	37.0
8	36.16367668097282	37.0	36.0	37.0	35.0	37.0
9	37.75903612303291	39.0	38.0	39.0	35.0	39.0
10	37.46161659513591	39.0	37.0	39.0	35.0	39.0
11	37.39454756795422	39.0	37.0	39.0	35.0	39.0
12	37.3350929899857	39.0	37.0	39.0	34.0	39.0
13	37.30246244635193	39.0	37.0	39.0	34.0	39.0
14	38.52812231759657	40.0	38.0	41.0	34.0	41.0
15	38.59358190271817	40.0	38.0	41.0	34.0	41.0
16	38.62442417739628	40.0	38.0	41.0	35.0	41.0
17	38.628147353361946	40.0	38.0	41.0	35.0	41.0
18	38.576169527896994	40.0	38.0	41.0	35.0	41.0
19	38.544273962804006	40.0	38.0	41.0	34.0	41.0
20	38.4463751788269	40.0	38.0	41.0	34.0	41.0
21	38.38819027181688	40.0	37.0	41.0	34.0	41.0
22	38.37439735336194	40.0	37.0	41.0	34.0	41.0
23	38.34453147353362	40.0	37.0	41.0	34.0	41.0
24	38.338830472103005	40.0	37.0	41.0	34.0	41.0
25	38.301199928469245	40.0	37.0	41.0	34.0	41.0
26	38.18017882689556	40.0	37.0	41.0	34.0	41.0
27	38.12717989985694	40.0	37.0	41.0	34.0	41.0
28	38.058102646638055	40.0	37.0	41.0	34.0	41.0
29	38.01010729613734	40.0	37.0	41.0	34.0	41.0
30	37.983370886981405	40.0	37.0	41.0	34.0	41.0
31	37.88536838340487	40.0	37.0	41.0	33.0	41.0
32	37.7539234620887	40.0	36.0	41.0	33.0	41.0
33	37.656444921316165	40.0	36.0	41.0	33.0	41.0
34	37.52704577968527	40.0	36.0	41.0	33.0	41.0
35	37.48660586552217	40.0	36.0	41.0	33.0	41.0
36	37.35611409155937	40.0	36.0	41.0	32.0	41.0
37	37.328283261802575	40.0	36.0	41.0	32.0	41.0
38	37.23075286123033	40.0	35.0	41.0	32.0	41.0
39	37.14567954220315	40.0	35.0	41.0	32.0	41.0
40	37.055608011444924	39.0	35.0	41.0	31.0	41.0
41	36.938068669527894	39.0	35.0	41.0	31.0	41.0
42	36.90045600858369	39.0	35.0	41.0	31.0	41.0
43	36.837135193133044	39.0	35.0	41.0	31.0	41.0
44	36.61575643776824	39.0	35.0	41.0	31.0	41.0
45	36.53476573676681	39.0	35.0	41.0	30.0	41.0
46	36.429318669527895	39.0	35.0	41.0	30.0	41.0
47	36.33753040057225	39.0	35.0	41.0	30.0	41.0
48	36.2283226037196	39.0	35.0	41.0	30.0	41.0
49	36.148172389127325	39.0	35.0	40.0	30.0	41.0
50	36.07031652360515	38.0	35.0	40.0	29.0	41.0
51	34.847839771101576	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	4.0
11	3.0
12	3.0
13	7.0
14	10.0
15	15.0
16	19.0
17	45.0
18	79.0
19	177.0
20	322.0
21	545.0
22	816.0
23	1306.0
24	1907.0
25	2828.0
26	3679.0
27	4783.0
28	5541.0
29	6387.0
30	7998.0
31	10029.0
32	12833.0
33	17272.0
34	30102.0
35	40379.0
36	39147.0
37	58522.0
38	107614.0
39	206680.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47782546494993	21.73730329041488	23.784334763948497	12.000536480686694
2	32.151108726752504	24.39717453505007	25.25447067238913	18.197246065808297
3	31.358905579399142	25.583869814020026	26.258583690987123	16.798640915593705
4	28.319384835479255	26.82868383404864	26.433297567954224	18.41863376251788
5	25.62356938483548	30.878397711015737	24.25214592274678	19.245886981402002
6	27.44438483547926	34.79524320457797	23.02521459227468	14.735157367668098
7	83.49087982832619	5.47925608011445	6.744635193133047	4.285228898426324
8	84.72567954220315	4.492131616595136	6.715307582260372	4.066881258941344
9	78.26251788268955	7.353898426323319	9.04595851216023	5.337625178826896
10	37.353004291845494	33.27038626609442	15.723533619456367	13.65307582260372
11	27.716022889842634	24.694742489270386	28.413805436337626	19.175429184549355
12	25.986945636623748	23.412374821173103	30.530042918454935	20.07063662374821
13	23.808834048640914	24.44098712446352	31.650035765379116	20.10014306151645
14	20.895922746781114	27.780758226037193	30.20976394849785	21.113555078683834
15	19.739985693848354	27.051502145922747	33.550786838340485	19.65772532188841
16	23.249284692417742	25.22353361945637	31.76859799713877	19.758583690987123
17	22.87750357653791	25.60783261802575	28.40504291845494	23.109620886981403
18	23.466201716738198	25.586909871244636	29.85371959942775	21.093168812589415
19	23.854434907010013	26.540593705293276	28.172925608011447	21.432045779685264
20	26.255364806866954	26.158440629470675	27.674892703862664	19.911301859799714
21	24.43258226037196	26.539878397711014	28.537017167381972	20.49052217453505
22	23.194742489270386	24.806330472103006	28.05937052932761	23.939556509299
23	21.4129113018598	27.063662374821174	28.945100143061513	22.57832618025751
24	22.53934191702432	25.10211015736767	29.552038626609445	22.80650929899857
25	22.06741773962804	27.11695278969957	27.648605150214596	23.167024320457795
26	20.852288984263232	28.891273247496425	28.102110157367665	22.154327610872677
27	22.412017167381972	27.40915593705293	28.425965665236053	21.75286123032904
28	20.335121602288982	28.605507868383405	29.236409155937054	21.82296137339056
29	22.085836909871244	27.12947067238913	28.275393419170243	22.509298998569385
30	22.715844062947067	27.092095851216026	27.605507868383405	22.586552217453505
31	22.762339055793994	28.30937052932761	26.218168812589415	22.710121602288986
32	22.33386981402003	28.628755364806867	27.019134477825464	22.01824034334764
33	21.478719599427755	28.242846924177396	26.75804721030043	23.52038626609442
34	21.437052932761087	27.98730329041488	27.739628040057223	22.83601573676681
35	21.038447782546495	27.390379113018597	27.5568669527897	24.014306151645208
36	21.7310443490701	29.233190271816884	25.66255364806867	23.37321173104435
37	22.199391988555078	28.68365522174535	27.107653791130186	22.009298998569385
38	20.742310443490698	27.864449213161656	26.646101573676678	24.747138769670958
39	22.384120171673818	25.43687410586552	27.220314735336192	24.958690987124463
40	23.290951359084406	24.684370529327612	28.39234620886981	23.632331902718168
41	19.76824034334764	27.649499284692418	27.10067954220315	25.481580829756794
42	21.330650929899857	27.623569384835477	27.092095851216026	23.953683834048643
43	21.71065808297568	26.940092989985693	27.383583690987123	23.965665236051503
44	21.570457796852647	27.01716738197425	27.666666666666668	23.74570815450644
45	21.489985693848354	25.464949928469245	27.428826895565095	25.61623748211731
46	21.926323319027183	26.211909871244636	27.298998569384835	24.562768240343345
47	21.180615164520745	26.545243204577968	28.80901287553648	23.465128755364805
48	20.760729613733904	25.38608726752504	28.414163090128756	25.4390200286123
49	21.406115879828327	25.578862660944207	28.742846924177396	24.272174535050073
50	19.69921316165951	25.519670958512158	28.662553648068666	26.118562231759658
51	20.092989985693848	25.164878397711014	27.437410586552218	27.304721030042916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	362.0
2	597.0
3	1584.0
4	2571.0
5	1811.0
6	1051.0
7	1008.5
8	966.0
9	947.5
10	929.0
11	980.0
12	1031.0
13	1016.0
14	1001.0
15	1059.0
16	1117.0
17	1160.0
18	1203.0
19	1202.0
20	1201.0
21	1406.0
22	1611.0
23	2119.5
24	2628.0
25	2960.0
26	4269.5
27	5247.0
28	6319.0
29	7391.0
30	8585.5
31	9780.0
32	10633.0
33	11486.0
34	13734.0
35	15982.0
36	17815.5
37	19649.0
38	21295.5
39	22942.0
40	25853.0
41	28764.0
42	31157.0
43	33550.0
44	36708.0
45	39866.0
46	43188.0
47	46510.0
48	50156.0
49	53802.0
50	52749.0
51	51696.0
52	45722.5
53	39749.0
54	36002.0
55	32255.0
56	28862.5
57	25470.0
58	24334.5
59	23199.0
60	22373.5
61	21548.0
62	19298.5
63	17049.0
64	14893.0
65	12737.0
66	10455.5
67	8174.0
68	6558.0
69	4942.0
70	4074.5
71	3207.0
72	2498.0
73	1789.0
74	1505.0
75	1107.5
76	994.0
77	696.5
78	399.0
79	309.0
80	219.0
81	185.0
82	151.0
83	102.0
84	53.0
85	40.5
86	28.0
87	21.5
88	15.0
89	10.0
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.55891329880147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37296619812919	24.287580978201074
2	9.082721624971233	6.096125350591178
3	3.2165525437784668	3.2383202381310277
4	1.766049784375737	2.370668463809296
5	1.275650128796384	2.140471603594139
6	1.0638690507728896	2.142137354169938
7	0.9355857412556016	2.1978068542074154
8	0.8823764648108088	2.3689276223591103
9	0.7818855434441827	2.3615306245826644
>10	8.541636594218419	49.56677866468286
>50	0.05398237662353241	1.2311636684599165
>100	0.024586032917644964	1.3050621870211803
>500	0.001603436929411628	0.44213555401418486
>1k	5.344789764705427E-4	0.2512908361760134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1401	0.2505364806866953	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	907	0.16219599427753933	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	868	0.15522174535050073	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT	690	0.12339055793991417	TruSeq Adapter, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.57653791130186E-4	0.0	0.0	0.1453862660944206	0.0
2	5.36480686695279E-4	0.0	0.0	0.6135550786838341	0.0
3	5.36480686695279E-4	0.0	0.0	0.920958512160229	0.0
4	5.36480686695279E-4	0.0	0.0	1.253934191702432	0.0
5	5.36480686695279E-4	0.0	0.0	2.234442060085837	0.0
6	5.36480686695279E-4	0.0	0.0	2.9427753934191703	0.0
7	5.36480686695279E-4	0.0	0.0	3.6278612303290414	0.0
8	5.36480686695279E-4	0.0	0.0	4.756437768240343	0.0
9	5.36480686695279E-4	0.0	0.0	5.245171673819742	0.0
10	5.36480686695279E-4	0.0	0.0	6.195636623748212	0.0
11	5.36480686695279E-4	0.0	0.0	7.426323319027182	0.0
12	5.36480686695279E-4	0.0	0.0	8.374821173104435	0.0
13	5.36480686695279E-4	0.0	0.0	8.792739628040056	0.0
14	5.36480686695279E-4	0.0	0.0	8.966022889842632	0.0
15	5.36480686695279E-4	0.0	0.0	9.257153075822604	0.0
16	5.36480686695279E-4	0.0	0.0	9.860515021459227	0.0
17	5.36480686695279E-4	0.0	0.0	10.619814020028612	0.0
18	5.36480686695279E-4	0.0	0.0	11.415057224606581	0.0
19	5.36480686695279E-4	0.0	0.0	11.920600858369099	0.0
20	5.36480686695279E-4	0.0	0.0	12.39467095851216	0.0
21	5.36480686695279E-4	0.0	0.0	12.978719599427754	0.0
22	5.36480686695279E-4	0.0	0.0	13.632868383404864	0.0
23	5.36480686695279E-4	0.0	0.0	14.273426323319027	0.0
24	5.36480686695279E-4	0.0	0.0	14.794170243204578	0.0
25	5.36480686695279E-4	0.0	0.0	15.24821173104435	0.0
26	7.15307582260372E-4	0.0	0.0	15.673640915593705	0.0
27	7.15307582260372E-4	0.0	0.0	16.103183118741057	0.0
28	7.15307582260372E-4	0.0	0.0	16.56777539341917	0.0
29	7.15307582260372E-4	0.0	0.0	17.04774678111588	0.0
30	7.15307582260372E-4	0.0	0.0	17.60675965665236	0.0
31	7.15307582260372E-4	0.0	0.0	18.10997854077253	0.0
32	7.15307582260372E-4	0.0	0.0	18.581008583690988	0.0
33	7.15307582260372E-4	0.0	0.0	19.079935622317596	0.0
34	7.15307582260372E-4	0.0	0.0	19.521638054363375	0.0
35	7.15307582260372E-4	0.0	0.0	19.99338340486409	0.0
36	7.15307582260372E-4	0.0	0.0	20.475679542203146	0.0
37	7.15307582260372E-4	0.0	0.0	20.948676680972817	0.0
38	7.15307582260372E-4	0.0	0.0	21.410228898426322	0.0
39	7.15307582260372E-4	0.0	0.0	21.87893419170243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGG	25	3.8882266E-5	45.000004	2
CGGACAT	20	7.030177E-4	45.0	36
GATCGCG	20	7.030177E-4	45.0	1
GTTAGCG	35	1.2104283E-7	45.0	1
CCATCGC	20	7.030177E-4	45.0	24
GCTACGA	55	1.8189894E-12	45.0	10
TGTAACG	40	6.8048394E-9	45.0	1
CGTAAGG	75	0.0	42.0	2
TTAACGG	70	0.0	41.785713	2
CTACGAA	60	3.6379788E-12	41.250004	11
CGAATAT	60	3.6379788E-12	41.250004	14
CGTTCAT	45	1.9252184E-8	40.0	17
CGTTAGG	80	0.0	39.375	2
GCGTACG	40	3.4539698E-7	39.375	1
CGTTTTT	845	0.0	38.609467	1
TCCGGCA	35	6.242537E-6	38.571426	24
GCGTTAG	70	0.0	38.571426	1
TACGAAT	65	9.094947E-12	38.07692	12
ACGGGCT	125	0.0	37.8	5
TAAGGCG	30	1.1391724E-4	37.500004	1
>>END_MODULE