FastQCFastQC Report
Sat 18 Jun 2016
SRR3550433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614636
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC12460.20272161083958634No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.1503328799484573No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT8600.13992021293904033No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC8130.13227341060399977No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6540.10640444100247952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG1150.045.0000042
GATAGCG302.1638461E-645.0000041
ATCTCGC207.0307223E-445.012
ACGTTAG253.888681E-545.01
ACGATAG253.888681E-545.01
AATTGCG207.0307223E-445.01
GCGATAT253.888681E-545.09
TATAGCG207.0307223E-445.01
AATAGCG207.0307223E-445.01
ACACGTG2600.041.53846442
TACGAAT1150.041.0869612
CACGTGA2600.040.6730843
AACACGT2700.040.00000441
GGTTACG451.925764E-840.01
GTATGCG403.4547156E-739.3751
TAGGGCG1950.039.230775
AACGAGC3150.038.5714315
TCACGAC356.2435447E-638.57142625
ATTAGCG356.2435447E-638.5714261
CGATTCG356.2435447E-638.57142610