FastQCFastQC Report
Sat 18 Jun 2016
SRR3550430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730776
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93771.2831565349710443No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT23650.3236285811247222No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC17970.24590298531971494No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT16920.23153469736280338No Hit
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG15810.21634536437978258No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC13980.19130349108345102TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT12170.16653529946248918TruSeq Adapter, Index 16 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT12140.16612477694943456No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC11370.15558803244769942TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC8190.11207264606391014No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC7350.10057801569838089No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG253.8894068E-545.01
CGTTTTT67150.043.994791
TACGAAT603.6379788E-1241.25000412
GCTACGA556.002665E-1140.90909210
TAGCCGT501.0804797E-940.544
TGTCGTG501.0804797E-940.51
TACGGGT1200.039.3750044
GTTTTTT75450.039.2743532
CGTTTCT3500.039.2142871
TAGCACG750.039.0000041
TCGTTAG356.2451636E-638.571431
CATACGA700.038.5714318
GCTAGCG356.2451636E-638.571431
GTATACG356.2451636E-638.571431
CGTTAGG1000.038.252
ACACGAC1650.038.18181626
CCGGGTC301.1395148E-437.5000045
CGCGCAA301.1395148E-437.50000418
TTCGTGA301.1395148E-437.50000411
GCGCAAT301.1395148E-437.50000419
Produced by FastQC (version 0.11.5)