FastQCFastQC Report
Sat 18 Jun 2016
SRR3550428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535849
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.5214155480368536No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG7060.1317535350443875No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC6250.11663733626450735TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG253.888009E-545.01
TGCCCGA207.0299115E-445.020
AATTGCG253.888009E-545.01
TACGAAT453.8380676E-1045.012
TTACGCG207.0299115E-445.01
GCTACGA453.8380676E-1045.010
GTACGAG406.8030204E-945.01
CGTTTTT17150.040.8017461
CCTCGAT501.0786607E-940.515
GCTAGCG451.9250365E-840.01
ATTGCGG1200.039.3750042
AACACGT2400.038.43750441
TCAAGCG2700.038.33333617
AACGGGT601.546141E-1037.5000044
AATACGG301.1391084E-437.5000042
AGTACGG900.037.52
CACGACC2750.036.81818427
CTACGAA552.743036E-936.8181811
GTACGGG2150.036.6279073
TGCGGGA4250.036.529414