##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535849
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.422732896767556	33.0	31.0	34.0	31.0	34.0
2	32.5431623461087	34.0	31.0	34.0	31.0	34.0
3	32.60885436009025	34.0	31.0	34.0	31.0	34.0
4	36.089937650345526	37.0	35.0	37.0	35.0	37.0
5	36.04847074455677	37.0	35.0	37.0	35.0	37.0
6	36.06474771810716	37.0	35.0	37.0	35.0	37.0
7	36.20558403580113	37.0	36.0	37.0	35.0	37.0
8	36.20726921203548	37.0	37.0	37.0	35.0	37.0
9	37.81300888869812	39.0	38.0	39.0	35.0	39.0
10	37.570405095465325	39.0	37.0	39.0	35.0	39.0
11	37.49353642537356	39.0	37.0	39.0	35.0	39.0
12	37.40143958465911	39.0	37.0	39.0	35.0	39.0
13	37.36358003840634	39.0	37.0	39.0	35.0	39.0
14	38.63177499631426	40.0	38.0	41.0	35.0	41.0
15	38.69348267888901	40.0	38.0	41.0	35.0	41.0
16	38.70298722214654	40.0	38.0	41.0	35.0	41.0
17	38.69299560137277	40.0	38.0	41.0	35.0	41.0
18	38.61421594516366	40.0	38.0	41.0	35.0	41.0
19	38.529298365770956	40.0	37.0	41.0	34.0	41.0
20	38.422327931936046	40.0	37.0	41.0	34.0	41.0
21	38.396012682677394	40.0	37.0	41.0	34.0	41.0
22	38.36921595449464	40.0	37.0	41.0	34.0	41.0
23	38.331470246282066	40.0	37.0	41.0	34.0	41.0
24	38.323796442654555	40.0	37.0	41.0	34.0	41.0
25	38.29104094623672	40.0	37.0	41.0	34.0	41.0
26	38.17832449066808	40.0	37.0	41.0	34.0	41.0
27	38.11761522369175	40.0	37.0	41.0	34.0	41.0
28	38.02998792570295	40.0	36.0	41.0	34.0	41.0
29	37.97539978613378	40.0	36.0	41.0	34.0	41.0
30	37.943120170047905	40.0	36.0	41.0	34.0	41.0
31	37.830739630007706	40.0	36.0	41.0	33.0	41.0
32	37.646473166880966	40.0	36.0	41.0	33.0	41.0
33	37.47794994485387	40.0	36.0	41.0	33.0	41.0
34	37.26745221135059	40.0	36.0	41.0	32.0	41.0
35	37.23555329953028	40.0	36.0	41.0	32.0	41.0
36	37.097370714510994	40.0	35.0	41.0	32.0	41.0
37	37.076663388379934	40.0	35.0	41.0	32.0	41.0
38	36.98077630078623	40.0	35.0	41.0	31.0	41.0
39	36.90796474379909	40.0	35.0	41.0	31.0	41.0
40	36.816560262312706	39.0	35.0	41.0	31.0	41.0
41	36.709832434137226	39.0	35.0	41.0	31.0	41.0
42	36.64704049088456	39.0	35.0	41.0	31.0	41.0
43	36.593549675374966	39.0	35.0	41.0	31.0	41.0
44	36.4351244473723	39.0	35.0	41.0	30.0	41.0
45	36.368101834658646	39.0	35.0	41.0	30.0	41.0
46	36.282404184761006	39.0	35.0	41.0	30.0	41.0
47	36.19932294359045	39.0	35.0	41.0	30.0	41.0
48	36.08759557263333	39.0	35.0	41.0	30.0	41.0
49	35.99959690136587	38.0	35.0	40.0	30.0	41.0
50	35.92321904118511	38.0	35.0	40.0	29.0	41.0
51	34.73856067660852	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	7.0
13	3.0
14	12.0
15	11.0
16	38.0
17	51.0
18	94.0
19	216.0
20	346.0
21	546.0
22	792.0
23	1301.0
24	1969.0
25	3181.0
26	4683.0
27	5737.0
28	6004.0
29	6639.0
30	7541.0
31	9046.0
32	11556.0
33	15838.0
34	29051.0
35	39731.0
36	35547.0
37	53012.0
38	99221.0
39	203516.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.058581801962866	21.140470543007453	24.574646962110595	13.226300692919088
2	33.46222536572803	23.753893354284507	25.36572803159099	17.41815324839647
3	31.65145404768881	23.81025251516752	28.241164955052632	16.297128482091036
4	28.22212974177427	25.636886510938716	27.704259968759853	18.43672377852716
5	25.701270320556723	30.274573620553554	25.084492086390007	18.939663972499716
6	26.718161273045204	34.240616293022846	24.209058895323125	14.832163538608825
7	81.74504384630745	5.523011146796952	8.551849494913679	4.1800955119819205
8	83.7409419444657	4.367088489481179	8.052828315439612	3.8391412506135127
9	76.99202573859426	7.061690886798333	10.23982502533363	5.7064583492737695
10	38.81130691668735	31.012094825221286	16.74762852967907	13.428969728412296
11	29.13563335939789	24.859242062595992	28.02487267868373	17.980251899322383
12	27.881548719881906	22.52556223861573	30.10437641947638	19.48851262202598
13	24.42572441116807	23.000136232408757	31.511489244171397	21.06265011225177
14	21.047160673995847	26.20607671190951	31.404929373760144	21.3418332403345
15	21.277822670192535	25.579594251365588	33.648658484013225	19.493924594428655
16	24.65937232317313	24.675608240381152	31.37096458143992	19.294054855005793
17	23.647146864135234	24.90552375762575	29.479573536574673	21.96775584166435
18	24.692030777327194	25.458478041388528	28.877911501187835	20.971579680096443
19	24.103432123601984	27.486288114748746	27.729453633392993	20.680826128256278
20	27.163435967968585	25.36218225656855	27.69511560159672	19.779266173866144
21	25.587059040886516	26.74914014955706	27.683731797577305	19.980069011979122
22	24.24843566004602	24.297143411670078	28.695024157925086	22.759396770358816
23	23.65461165365616	26.539006324542925	28.544795268816404	21.261586752984517
24	23.672340528768366	24.608051895216747	29.878006677254227	21.841600898760657
25	23.31309753307368	26.10884782839942	27.66152404875254	22.916530589774357
26	22.749319304505562	26.773587335238098	28.790200224316926	21.686893135939417
27	22.923995379295288	26.119485153466744	28.622988939048128	22.333530528189844
28	21.61056566308792	27.72814729522683	29.07871433930081	21.58257270238444
29	23.585562350587573	25.76322807358043	28.595369217820693	22.055840358011306
30	24.571661046302225	24.533963859221533	29.16511927800556	21.729255816470687
31	24.15307297391616	26.306664750704023	27.639129680189754	21.901132595190063
32	25.54413650114118	26.08104148743396	28.00341140881106	20.371410602613796
33	24.795418112192056	26.967485243044216	27.11192892027418	21.12516772448955
34	23.388305287497037	26.636795067267084	29.393728457083988	20.581171188151888
35	23.941259571259813	26.820802128957972	27.93641492286073	21.301523376921484
36	22.965611580874462	29.56392565816116	26.676731691204054	20.793731069760323
37	24.994914612138867	27.961795207231887	26.580062666908027	20.463227513721215
38	22.952361579474815	28.194510020546833	26.639407743599406	22.213720656378943
39	24.352196234386927	26.08272106507617	26.811657761794834	22.75342493874207
40	24.138703254088373	25.65088299129046	28.93856291604538	21.271850838575794
41	21.5372241060448	27.1386155428115	28.255721294618446	23.068439056525254
42	22.45893899214144	26.894330305739118	27.613002916866503	23.033727785252935
43	22.94564326890598	26.36936898267982	27.76360504545124	22.921382702962962
44	23.40939331789366	26.21522107907265	27.555337417817334	22.820048185216358
45	23.220907382490218	24.959270242176434	27.96907337701479	23.850748998318554
46	23.03111510892061	26.840210581712387	27.064714126554307	23.063960182812696
47	22.728044654370912	26.27904502947659	28.90627770136736	22.086632614785135
48	22.7842171955159	24.68773852335266	28.987270667669435	23.540773613462
49	22.720953104326032	25.252076611134854	28.70790091984869	23.319069364690424
50	21.11061138492374	25.68428792439661	29.32038690004087	23.88471379063878
51	21.194776886772207	25.024960389960604	28.623362178524175	25.156900544743017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	376.0
2	601.0
3	2947.5
4	5294.0
5	3539.5
6	1785.0
7	1785.0
8	1785.0
9	1724.0
10	1663.0
11	1617.5
12	1572.0
13	1589.0
14	1606.0
15	1506.0
16	1406.0
17	1396.5
18	1387.0
19	1363.0
20	1339.0
21	1507.0
22	1675.0
23	1901.5
24	2128.0
25	2647.0
26	3774.0
27	4382.0
28	5417.0
29	6452.0
30	7772.5
31	9093.0
32	10554.0
33	12015.0
34	13556.0
35	15097.0
36	16233.5
37	17370.0
38	18662.5
39	19955.0
40	23033.0
41	26111.0
42	27132.5
43	28154.0
44	30315.5
45	32477.0
46	36508.0
47	40539.0
48	42744.5
49	44950.0
50	44706.5
51	44463.0
52	41001.5
53	37540.0
54	34539.0
55	31538.0
56	30187.0
57	28836.0
58	27850.0
59	26864.0
60	26343.0
61	25822.0
62	23723.5
63	21625.0
64	17844.5
65	14064.0
66	11212.0
67	8360.0
68	6990.5
69	5621.0
70	4371.0
71	3121.0
72	2781.5
73	2442.0
74	2000.5
75	1254.5
76	950.0
77	717.5
78	485.0
79	332.0
80	179.0
81	148.0
82	117.0
83	92.0
84	67.0
85	50.5
86	34.0
87	18.5
88	3.0
89	4.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	535849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.61458156985333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.90944300695395	23.16362092890413
2	7.885413723652908	5.301297656515432
3	3.776553476025503	3.808417946183172
4	2.6078949719637485	3.506531930427432
5	2.21044838549039	3.715164878000866
6	1.8914302202289301	3.814778125294261
7	1.6884229528846124	3.97289417519067
8	1.4900287360373066	4.00693539911612
9	1.319203386269313	3.991002285147891
>10	8.161488460305371	41.77691091173153
>50	0.03959589059202871	0.8928943152074776
>100	0.017846035196404878	1.1789654311020847
>500	0.0016730657996629572	0.34680967523276457
>1k	5.576885998876524E-4	0.523776341946132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2794	0.5214155480368536	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	706	0.1317535350443875	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	625	0.11663733626450735	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09293662953555946	0.0
2	0.0	0.0	0.0	0.3689472220718897	0.0
3	0.0	0.0	0.0	0.6475704909405449	0.0
4	0.0	0.0	0.0	0.892788826703045	0.0
5	0.0	0.0	0.0	1.5672325599189323	0.0
6	0.0	0.0	0.0	2.2980354540178296	0.0
7	0.0	0.0	0.0	2.9097749552579177	0.0
8	0.0	0.0	0.0	3.901472243113265	0.0
9	0.0	0.0	0.0	4.421954692460003	0.0
10	0.0	0.0	0.0	5.174778715645639	0.0
11	0.0	0.0	0.0	5.888599213584424	0.0
12	0.0	0.0	0.0	6.504630968799046	0.0
13	0.0	0.0	0.0	6.784000716619794	0.0
14	0.0	0.0	0.0	6.938148620226967	0.0
15	0.0	0.0	0.0	7.140631035982151	0.0
16	0.0	0.0	0.0	7.478786001280211	0.0
17	0.0	0.0	0.0	7.882631114362441	0.0
18	0.0	0.0	0.0	8.301032567010482	0.0
19	0.0	0.0	0.0	8.589546681994367	0.0
20	0.0	0.0	0.0	8.862944598198373	0.0
21	0.0	0.0	0.0	9.21304322672992	0.0
22	0.0	0.0	0.0	9.603451718674478	0.0
23	0.0	0.0	0.0	9.975571476292762	0.0
24	0.0	0.0	0.0	10.291145453290012	0.0
25	0.0	0.0	0.0	10.566036327398203	0.0
26	0.0	0.0	0.0	10.83943424360221	0.0
27	0.0	0.0	0.0	11.108353286093656	0.0
28	0.0	0.0	0.0	11.401532894528122	0.0
29	0.0	0.0	0.0	11.692286446368287	0.0
30	0.0	0.0	0.0	12.051716061800992	0.0
31	3.732394760464235E-4	0.0	0.0	12.36411750325185	0.0
32	3.732394760464235E-4	0.0	0.0	12.679878099987123	0.0
33	3.732394760464235E-4	0.0	0.0	12.971564750517404	0.0
34	3.732394760464235E-4	0.0	0.0	13.275195064281169	0.0
35	3.732394760464235E-4	0.0	0.0	13.595247914990978	0.0
36	3.732394760464235E-4	0.0	0.0	13.935269077669268	0.0
37	3.732394760464235E-4	0.0	0.0	14.279582494322094	0.0
38	3.732394760464235E-4	0.0	0.0	14.678948733691767	0.0
39	5.598592140696353E-4	0.0	0.0	15.047522716287611	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.888009E-5	45.0	1
TGCCCGA	20	7.0299115E-4	45.0	20
AATTGCG	25	3.888009E-5	45.0	1
TACGAAT	45	3.8380676E-10	45.0	12
TTACGCG	20	7.0299115E-4	45.0	1
GCTACGA	45	3.8380676E-10	45.0	10
GTACGAG	40	6.8030204E-9	45.0	1
CGTTTTT	1715	0.0	40.801746	1
CCTCGAT	50	1.0786607E-9	40.5	15
GCTAGCG	45	1.9250365E-8	40.0	1
ATTGCGG	120	0.0	39.375004	2
AACACGT	240	0.0	38.437504	41
TCAAGCG	270	0.0	38.333336	17
AACGGGT	60	1.546141E-10	37.500004	4
AATACGG	30	1.1391084E-4	37.500004	2
AGTACGG	90	0.0	37.5	2
CACGACC	275	0.0	36.818184	27
CTACGAA	55	2.743036E-9	36.81818	11
GTACGGG	215	0.0	36.627907	3
TGCGGGA	425	0.0	36.52941	4
>>END_MODULE