FastQCFastQC Report
Sat 18 Jun 2016
SRR3550426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848739
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98441.159838301291681No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC49530.5835716280269907No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG19570.23057736241647903No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT19370.22822092539638217No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC18890.22256547654814968No Hit
CGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG13700.16141593587663583No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC13320.15693870553845174No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT13260.1562317744324227No Hit
CGTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC12990.1530505844552919No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT12250.1443317674809335No Hit
GAATGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT10000.11782185100484366No Hit
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC9620.1133446206666596No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT9290.10945649958349975Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT9260.10910303403048523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG253.8899394E-545.0000041
TTTGCGC302.1648284E-644.99999613
CGTTTTT55800.043.0241971
AATTGCG650.041.538461
TGCGTAG650.041.538461
CGACGGT603.6379788E-1241.24999628
TGTTGCG501.0804797E-940.5000041
AATAGCG403.4567893E-739.3751
TGCCGAC403.4567893E-739.37541
GCGTAGG1150.039.1304322
CGCGCCT356.2463605E-638.57142617
ATACTCG356.2463605E-638.57142618
CGAATAT1000.038.25000414
ACGTAAG659.094947E-1238.076921
GATAGCG601.546141E-1037.4999961
GACACGA4000.037.12500425
TACGGGA2850.037.1052634
GTTTTTT67450.036.8606342
CACGACC4050.036.66666827
ACGACCA4050.036.66666828
Produced by FastQC (version 0.11.5)