FastQCFastQC Report
Sat 18 Jun 2016
SRR3550425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675635
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.3307999141548321No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC9290.13750027751670651No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC7390.10937858459079237No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT6840.1012380945332909Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC207.0312206E-445.018
AATCGGA207.0312206E-445.010
ATGGGCG1600.040.781255
CGTAAGG800.039.3752
TGGGCGA3600.039.3756
CGACCCG750.039.00000432
CGATTCG356.244465E-638.5714310
TCGTAAG356.244465E-638.571431
TGTAACG356.244465E-638.571431
CGTTTTT12800.037.968751
TAGGGCG2500.037.85
GGCGTAG301.1394239E-437.4999961
TAATGCG552.744855E-936.818181
TCGTTGA250.002106495735.99999624
CGACGGT250.002106495735.99999628
ATCGTTG250.002106495735.99999623
CCCCGAT250.002106495735.99999640
TCTACGG250.002106495735.9999962
GTAGACG504.8738002E-835.9999961
CGGGCTA250.002106495735.9999966
Produced by FastQC (version 0.11.5)