##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675635
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32654317789931	33.0	31.0	34.0	30.0	34.0
2	32.430543118695745	34.0	31.0	34.0	31.0	34.0
3	32.528667105759766	34.0	31.0	34.0	31.0	34.0
4	36.02217913518394	37.0	35.0	37.0	35.0	37.0
5	35.98063895446506	37.0	35.0	37.0	35.0	37.0
6	35.995001739104694	37.0	35.0	37.0	35.0	37.0
7	36.14310389485447	37.0	35.0	37.0	35.0	37.0
8	36.124775951512284	37.0	36.0	37.0	35.0	37.0
9	37.69469018034886	39.0	38.0	39.0	35.0	39.0
10	37.44904275237369	39.0	37.0	39.0	35.0	39.0
11	37.41177558889045	39.0	37.0	39.0	35.0	39.0
12	37.336196319018406	39.0	37.0	39.0	35.0	39.0
13	37.28941366270249	39.0	37.0	39.0	34.0	39.0
14	38.55504081345697	40.0	38.0	41.0	34.0	41.0
15	38.610252577205145	40.0	38.0	41.0	34.0	41.0
16	38.60782967134622	40.0	38.0	41.0	34.0	41.0
17	38.62839994967697	40.0	38.0	41.0	35.0	41.0
18	38.570553627328366	40.0	38.0	41.0	35.0	41.0
19	38.52282519407668	40.0	38.0	41.0	34.0	41.0
20	38.397372841845076	40.0	37.0	41.0	34.0	41.0
21	38.35988958535304	40.0	37.0	41.0	34.0	41.0
22	38.31993902032902	40.0	37.0	41.0	34.0	41.0
23	38.27300391483568	40.0	37.0	41.0	34.0	41.0
24	38.24244451515981	40.0	37.0	41.0	34.0	41.0
25	38.19721151213303	40.0	37.0	41.0	34.0	41.0
26	38.07613134310685	40.0	37.0	41.0	34.0	41.0
27	37.99862499722483	40.0	37.0	41.0	34.0	41.0
28	37.92583125504155	40.0	37.0	41.0	33.0	41.0
29	37.86438387590933	40.0	36.0	41.0	33.0	41.0
30	37.82525476033657	40.0	36.0	41.0	33.0	41.0
31	37.73800646798937	40.0	36.0	41.0	33.0	41.0
32	37.57257098877352	40.0	36.0	41.0	33.0	41.0
33	37.455929606962336	40.0	36.0	41.0	33.0	41.0
34	37.30979596971738	40.0	36.0	41.0	32.0	41.0
35	37.27594041161278	40.0	36.0	41.0	32.0	41.0
36	37.13806123128612	40.0	35.0	41.0	32.0	41.0
37	37.09443560502342	40.0	35.0	41.0	31.0	41.0
38	36.99401007940678	39.0	35.0	41.0	31.0	41.0
39	36.9013550215723	39.0	35.0	41.0	31.0	41.0
40	36.85967719256699	39.0	35.0	41.0	31.0	41.0
41	36.73381485565431	39.0	35.0	41.0	31.0	41.0
42	36.66936437573542	39.0	35.0	41.0	31.0	41.0
43	36.60397255914806	39.0	35.0	41.0	31.0	41.0
44	36.43814781649856	39.0	35.0	41.0	30.0	41.0
45	36.37990779044898	39.0	35.0	41.0	30.0	41.0
46	36.295014319862055	39.0	35.0	41.0	30.0	41.0
47	36.21938028669326	39.0	35.0	41.0	30.0	41.0
48	36.08446276465843	38.0	35.0	40.0	30.0	41.0
49	36.00504710383565	38.0	35.0	40.0	29.0	41.0
50	35.919224137293064	38.0	35.0	40.0	29.0	41.0
51	34.706767707415985	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	4.0
12	3.0
13	10.0
14	8.0
15	20.0
16	34.0
17	76.0
18	152.0
19	259.0
20	402.0
21	703.0
22	1088.0
23	1703.0
24	2485.0
25	3682.0
26	5317.0
27	6451.0
28	7372.0
29	8359.0
30	10049.0
31	12504.0
32	15635.0
33	21143.0
34	36350.0
35	48973.0
36	48777.0
37	72428.0
38	130454.0
39	241007.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74230908700704	20.9733065930569	24.679745720692388	11.604638599243675
2	32.304424726368524	24.651772036676608	24.96007459649071	18.083728640464155
3	32.2742309087007	24.20804132408771	26.330045068713137	17.18768269849845
4	29.10728425851236	26.114840113374825	25.535681248011134	19.242194380101683
5	26.06096487008518	30.39599783906991	23.718427849356534	19.824609441488377
6	26.809741946465177	35.14412367624531	22.982675557068536	15.063458820220976
7	81.79623613341523	5.913399986679198	7.806729965143902	4.483633914761668
8	82.60140460455719	5.250468078178306	7.874222028166096	4.273905289098404
9	75.61642010849053	7.869337734131594	10.120405248395953	6.39383690898192
10	38.6297335099573	28.009798189851033	17.41324827754631	15.947220022645364
11	29.45022090329838	25.63987952074715	25.39699689921333	19.512902676741138
12	26.467841364050116	24.154166080798063	28.75058278508366	20.62740977006816
13	23.63243467256729	24.76855106677422	31.100520251319132	20.498494009339364
14	21.640382751041614	26.61525823854596	29.88625515256018	21.858103857852242
15	21.309878854707055	26.313616079687996	31.4766108919757	20.899894173629253
16	24.084453884123825	25.677177766101515	29.852213103228813	20.386155246545844
17	23.75913029964404	25.094170669074277	28.387368919608964	22.759330111672725
18	24.35723430550519	25.563358914206635	28.83228370347895	21.247123076809224
19	25.207841512059026	26.35772273490864	26.950054393274474	21.484381359757858
20	25.960170802282295	26.03432326626063	27.497391342958842	20.508114588498227
21	25.219090189229394	26.74772621311803	27.568139602004038	20.46504399564854
22	24.0526319684445	24.896134747311788	27.501091565712255	23.550141718531457
23	22.865600509150653	26.85710479770882	27.933721610040923	22.343573083099603
24	22.624938021268882	25.660896785986516	29.082566770519584	22.631598422225018
25	23.004432866858586	27.167183464444562	26.910832032088333	22.917551636608525
26	22.338984806885374	27.37084372479223	27.571247789116903	22.71892367920549
27	23.21623361726376	26.932441332968242	27.29683926972404	22.554485780043958
28	21.854255626188696	27.19693325538197	28.221598940256204	22.727212178173126
29	22.479149244784537	26.007681662436077	27.70075558548625	23.81241350729314
30	23.040103014201456	26.082130144234682	27.520332724029988	23.357434117533877
31	23.983067780680397	26.966187364479342	25.6132379169226	23.43750693791766
32	23.42403812709525	26.73218527755371	26.8964751678051	22.947301427545938
33	22.905562914887476	27.50049953007171	26.341589763703777	23.252347791337037
34	22.15515774049598	26.78709658321431	27.59285708999682	23.464888586292894
35	22.477669155683174	26.206457628749252	27.266497443146076	24.0493757724215
36	23.238138935963946	28.530049508980444	26.09826311543955	22.133548439616067
37	23.15273779481525	27.790152967208627	26.962487141725934	22.094622096250195
38	22.645363250867703	27.753594766404937	27.215730386969295	22.385311595758065
39	22.701902654539804	26.103887454024733	28.034959704574213	23.15925018686125
40	23.36128234919742	25.66637311566156	28.018086688818666	22.95425784632235
41	21.11820731608043	26.64426798493269	27.60884205229155	24.62868264669533
42	22.83614673603351	26.43601944837079	27.807322000784445	22.92051181481125
43	22.81157725695087	25.273557468159584	28.71254449517861	23.202320779710938
44	22.38013128390329	25.932789153907066	27.561923227778312	24.125156334411333
45	22.4838855299089	25.396256854662653	27.346126236799456	24.773731378628995
46	22.54590126325605	26.52393674099181	27.137877700237556	23.79228429551459
47	22.33883679797524	25.53153699852731	28.807566215486176	23.32205998801128
48	22.038378710398366	25.13102488769824	29.0252873222968	23.805309079606594
49	22.628786252932425	25.245287766323532	28.41771074618692	23.70821523455712
50	21.3621259999852	24.774175405359404	29.349574844405634	24.514123750249762
51	21.274208707364185	24.856912386125646	27.713040325027567	26.155838581482605
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	190.0
1	617.0
2	1044.0
3	2618.5
4	4193.0
5	2804.0
6	1415.0
7	1429.0
8	1443.0
9	1444.5
10	1446.0
11	1468.5
12	1491.0
13	1474.5
14	1458.0
15	1486.5
16	1515.0
17	1510.5
18	1506.0
19	1719.5
20	1933.0
21	1989.5
22	2046.0
23	2165.0
24	2284.0
25	2894.0
26	4347.5
27	5191.0
28	6603.0
29	8015.0
30	9187.0
31	10359.0
32	11686.0
33	13013.0
34	14779.0
35	16545.0
36	18884.0
37	21223.0
38	22859.0
39	24495.0
40	27545.5
41	30596.0
42	33401.0
43	36206.0
44	39687.0
45	43168.0
46	49415.5
47	55663.0
48	59625.0
49	63587.0
50	62448.5
51	61310.0
52	56416.0
53	51522.0
54	46678.5
55	41835.0
56	38835.5
57	35836.0
58	33311.5
59	30787.0
60	29051.5
61	27316.0
62	25033.0
63	22750.0
64	20034.0
65	17318.0
66	14567.5
67	11817.0
68	9914.0
69	8011.0
70	6626.0
71	5241.0
72	4333.5
73	3426.0
74	2877.0
75	1815.5
76	1303.0
77	1041.0
78	779.0
79	522.0
80	265.0
81	210.5
82	156.0
83	113.5
84	71.0
85	46.0
86	21.0
87	15.5
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.33260589338561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47396755933251	23.109396247325265
2	8.489305664446272	5.489627487140548
3	3.205401983603141	3.1091699719715056
4	1.9246340602718317	2.4891373823902283
5	1.4607126756591218	2.3614323632779595
6	1.215034718690734	2.3571143223724897
7	1.0989835790469895	2.4873102061240098
8	1.0025752523571678	2.593269641034075
9	0.9847937944900217	2.865685467914789
>10	9.042952096775762	48.95867991828393
>50	0.07128500170522707	1.6160574828811356
>100	0.027134290964461537	1.6374700955060484
>500	0.0027594194201147324	0.5933078170240768
>1k	4.599032366857888E-4	0.3323415967540881
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2235	0.3307999141548321	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	929	0.13750027751670651	No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	739	0.10937858459079237	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	684	0.1012380945332909	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1150029231759752	0.0
2	0.0	0.0	0.0	0.47584864608849453	0.0
3	0.0	0.0	0.0	0.717251178520947	0.0
4	0.0	0.0	0.0	1.0342862640330948	0.0
5	0.0	0.0	0.0	1.9042826378147963	0.0
6	0.0	0.0	0.0	2.6651964448259786	0.0
7	0.0	0.0	0.0	3.3045949366151843	0.0
8	0.0	0.0	0.0	4.365966831203238	0.0
9	0.0	0.0	0.0	4.846255744595824	0.0
10	0.0	0.0	0.0	5.6829501135968385	0.0
11	0.0	0.0	0.0	6.6931109252777015	0.0
12	0.0	0.0	0.0	7.502127628083211	0.0
13	0.0	0.0	0.0	7.847728433251682	0.0
14	0.0	0.0	0.0	8.030519437270124	0.0
15	0.0	0.0	0.0	8.301819769550127	0.0
16	0.0	0.0	0.0	8.824587240151857	0.0
17	0.0	0.0	0.0	9.418250978708919	0.0
18	0.0	0.0	0.0	10.039740392371622	0.0
19	0.0	0.0	0.0	10.454017331843376	0.0
20	0.0	0.0	0.0	10.854825460492721	0.0
21	0.0	0.0	0.0	11.361607968799722	0.0
22	1.480089101363902E-4	0.0	0.0	11.905096686820547	0.0
23	1.480089101363902E-4	0.0	0.0	12.43718871876087	0.0
24	1.480089101363902E-4	0.0	0.0	12.889503948137678	0.0
25	1.480089101363902E-4	0.0	0.0	13.299636638125616	0.0
26	1.480089101363902E-4	0.0	0.0	13.667290770904408	0.0
27	1.480089101363902E-4	0.0	0.0	14.03834910861634	0.0
28	1.480089101363902E-4	0.0	0.0	14.41384771363236	0.0
29	1.480089101363902E-4	0.0	0.0	14.825756510541934	0.0
30	1.480089101363902E-4	0.0	0.0	15.298644978427701	0.0
31	1.480089101363902E-4	0.0	0.0	15.732607102947597	0.0
32	1.480089101363902E-4	0.0	0.0	16.164053075995174	0.0
33	1.480089101363902E-4	0.0	0.0	16.558792839328927	0.0
34	1.480089101363902E-4	0.0	0.0	16.95072043337009	0.0
35	1.480089101363902E-4	0.0	0.0	17.38113034404671	0.0
36	2.960178202727804E-4	0.0	0.0	17.798515470631333	0.0
37	2.960178202727804E-4	0.0	0.0	18.213384445743635	0.0
38	2.960178202727804E-4	0.0	0.0	18.605312039784796	0.0
39	5.920356405455608E-4	0.0	0.0	18.99871972292732	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGATC	20	7.0312206E-4	45.0	18
AATCGGA	20	7.0312206E-4	45.0	10
ATGGGCG	160	0.0	40.78125	5
CGTAAGG	80	0.0	39.375	2
TGGGCGA	360	0.0	39.375	6
CGACCCG	75	0.0	39.000004	32
CGATTCG	35	6.244465E-6	38.57143	10
TCGTAAG	35	6.244465E-6	38.57143	1
TGTAACG	35	6.244465E-6	38.57143	1
CGTTTTT	1280	0.0	37.96875	1
TAGGGCG	250	0.0	37.8	5
GGCGTAG	30	1.1394239E-4	37.499996	1
TAATGCG	55	2.744855E-9	36.81818	1
TCGTTGA	25	0.0021064957	35.999996	24
CGACGGT	25	0.0021064957	35.999996	28
ATCGTTG	25	0.0021064957	35.999996	23
CCCCGAT	25	0.0021064957	35.999996	40
TCTACGG	25	0.0021064957	35.999996	2
GTAGACG	50	4.8738002E-8	35.999996	1
CGGGCTA	25	0.0021064957	35.999996	6
>>END_MODULE