FastQCFastQC Report
Sat 18 Jun 2016
SRR3550419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507144
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC13040.2571261811240989No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC12160.23977410755130693No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT9610.18949253072105754No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.15892921931443535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAG351.2101373E-745.0000041
TCCGCGC207.0295535E-445.017
GTCGACT207.0295535E-445.042
AGATACG207.0295535E-445.01
GTTAGCG207.0295535E-445.01
ATTCGCA207.0295535E-445.039
TTGTAAC207.0295535E-445.022
GTATGCG207.0295535E-445.01
CTTTGCG253.8877115E-545.01
GTATCGG207.0295535E-445.02
GCGATAT207.0295535E-445.09
ACGCTAG207.0295535E-445.01
ACTCGAT207.0295535E-445.010
CTATTCG207.0295535E-445.042
CGTCGAC207.0295535E-445.041
TTCGCAT207.0295535E-445.040
TGTGTCG207.0295535E-445.030
GTGCGCC207.0295535E-445.019
TAATGCG207.0295535E-445.024
GCGTACG453.8380676E-1045.01