FastQCFastQC Report
Sat 18 Jun 2016
SRR3550418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371679
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC8580.23084435763118175No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.1740749410109261No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC6010.16169867008897465No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT5400.1452866586489955Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4090.11004119145822068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG302.1615233E-645.0000041
CATAAGG302.1615233E-645.0000042
CGATGCG302.1615233E-645.00000410
TAATGCG302.1615233E-645.00000424
GGTCGAC207.0271204E-445.020
GCAACCC253.8856942E-545.022
ATTACGG253.8856942E-545.02
CAATTGG207.0271204E-445.02
ACGGGCC207.0271204E-445.05
CTAACGG351.2089549E-745.02
CGGGCTA253.8856942E-545.06
AGCGATA207.0271204E-445.042
ATGCGAG253.8856942E-545.01
AATAGCG207.0271204E-445.01
TGGTCGA207.0271204E-445.019
TACGTAA207.0271204E-445.017
TAGCATA1250.041.430
ATGGGCG1150.041.0869565
GGCGATA1100.040.909098
AGTACGG556.002665E-1140.909092
Produced by FastQC (version 0.11.5)