##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371679
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29661616610032	33.0	31.0	34.0	30.0	34.0
2	32.40621611659523	34.0	31.0	34.0	30.0	34.0
3	32.511817993483625	34.0	31.0	34.0	31.0	34.0
4	35.99220294931917	37.0	35.0	37.0	35.0	37.0
5	35.96107393745678	37.0	35.0	37.0	35.0	37.0
6	35.96848624754156	37.0	35.0	37.0	35.0	37.0
7	36.129700628768376	37.0	35.0	37.0	35.0	37.0
8	36.115371059435695	37.0	35.0	37.0	35.0	37.0
9	37.72288991307015	39.0	38.0	39.0	35.0	39.0
10	37.458139954100176	39.0	37.0	39.0	35.0	39.0
11	37.35701505869312	39.0	37.0	39.0	35.0	39.0
12	37.17474218344324	39.0	37.0	39.0	34.0	39.0
13	37.074365245278855	39.0	37.0	39.0	33.0	39.0
14	38.24953521721701	40.0	37.0	41.0	33.0	41.0
15	38.348139120047136	40.0	37.0	41.0	34.0	41.0
16	38.397883657672345	40.0	37.0	41.0	34.0	41.0
17	38.396089098388664	40.0	37.0	41.0	34.0	41.0
18	38.35110673457473	40.0	37.0	41.0	34.0	41.0
19	38.321027015247026	40.0	37.0	41.0	34.0	41.0
20	38.20306770089244	40.0	37.0	41.0	34.0	41.0
21	38.142620379413415	40.0	36.0	41.0	34.0	41.0
22	38.119759254625635	40.0	36.0	41.0	34.0	41.0
23	38.086235703389214	40.0	36.0	41.0	34.0	41.0
24	38.055765862478104	40.0	36.0	41.0	34.0	41.0
25	37.98149209398432	40.0	36.0	41.0	34.0	41.0
26	37.867463590894296	40.0	36.0	41.0	34.0	41.0
27	37.806978602503776	40.0	36.0	41.0	33.0	41.0
28	37.719892703112095	40.0	36.0	41.0	33.0	41.0
29	37.651747879218355	40.0	36.0	41.0	33.0	41.0
30	37.62360531533931	40.0	36.0	41.0	33.0	41.0
31	37.498432787432165	40.0	35.0	41.0	33.0	41.0
32	37.36506501577975	40.0	35.0	41.0	33.0	41.0
33	37.26682163910256	39.0	35.0	41.0	32.0	41.0
34	37.08913067458748	39.0	35.0	41.0	32.0	41.0
35	37.08300442048112	39.0	35.0	41.0	32.0	41.0
36	36.936679231272144	39.0	35.0	41.0	31.0	41.0
37	36.913481794774526	39.0	35.0	41.0	31.0	41.0
38	36.82594927343218	39.0	35.0	41.0	31.0	41.0
39	36.74767205034452	39.0	35.0	41.0	31.0	41.0
40	36.67906446153805	39.0	35.0	41.0	31.0	41.0
41	36.525832775055896	39.0	35.0	41.0	31.0	41.0
42	36.45311411190839	39.0	35.0	41.0	31.0	41.0
43	36.36509192071653	39.0	35.0	41.0	30.0	41.0
44	36.18600189948854	38.0	35.0	41.0	30.0	41.0
45	36.13406730000888	38.0	35.0	40.0	30.0	41.0
46	36.02667893531784	38.0	35.0	40.0	30.0	41.0
47	35.932955588020846	38.0	35.0	40.0	30.0	41.0
48	35.8083884211914	38.0	35.0	40.0	29.0	41.0
49	35.72466832939176	38.0	35.0	40.0	29.0	41.0
50	35.62662673974047	37.0	35.0	40.0	29.0	41.0
51	34.3678227718004	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	5.0
12	6.0
13	9.0
14	8.0
15	12.0
16	28.0
17	43.0
18	90.0
19	159.0
20	306.0
21	424.0
22	750.0
23	1012.0
24	1493.0
25	2144.0
26	2719.0
27	3378.0
28	3886.0
29	4667.0
30	5714.0
31	7205.0
32	9206.0
33	12798.0
34	23818.0
35	33682.0
36	27049.0
37	40311.0
38	68055.0
39	122612.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.315815528991415	20.62747693574294	25.872594362339544	11.1841131729261
2	31.50487382929894	24.60402659283952	25.688564594717484	18.202534983144055
3	32.291843230314335	24.56555253323432	25.99070703483382	17.151897201617526
4	28.834827902571842	25.70336230995025	26.085950511059274	19.37585927641863
5	26.16612722268409	31.07170434703064	23.35267798288308	19.409490447402195
6	26.847360222127158	34.783509426144605	22.615751764291232	15.753378587437009
7	82.06597628598871	6.197552188851133	7.228549366523264	4.507922158636888
8	82.87796727821588	5.1216237667449604	7.235813699455713	4.764595255583447
9	76.67664839821458	7.250342365320613	9.852049752609107	6.220959483855692
10	43.63442648091498	23.8181333892956	16.196502896316446	16.35093723347297
11	33.15710599737946	23.928712679489557	23.033854481958898	19.880326841172085
12	30.33235668412797	22.731981091210425	26.535801054135423	20.39986117052618
13	25.43646533702469	24.009427489850115	28.70756755157004	21.84653962155516
14	21.15481369676522	26.996413571926315	28.673667331218606	23.175105400089862
15	20.256188808084396	24.899711848127012	33.29136163194585	21.552737711842745
16	22.69350703160523	23.872212312237174	30.931798675739014	22.502481980418587
17	22.333249928029293	23.689258742086587	28.268748032576497	25.708743297307624
18	24.3188342628989	24.47972578488427	28.76326077071882	22.438179181498015
19	24.827606617538255	26.04855264892555	25.455836891511225	23.668003842024973
20	27.942122099984125	25.286335789754062	25.59681876027432	21.174723349987488
21	25.207773374336455	26.77660023837774	26.082452869276985	21.933173518008818
22	24.138840235794866	24.44286602148628	25.623454647693304	25.794839095025544
23	24.238119452538346	26.525039079420683	25.70766709983615	23.52917436820482
24	25.060334320744516	23.4234379666325	28.025527404023364	23.490700308599624
25	23.369897142426662	25.334495626602525	25.93904955620307	25.356557674767743
26	22.2904710785382	27.12905491028549	26.099133930084832	24.481340081091478
27	22.528579769101835	26.765838263663	26.26379214322036	24.44178982401481
28	21.66573844634752	27.192819610470327	27.252279520769267	23.889162422412888
29	23.489086012392413	25.056029530858616	26.827719618272756	24.62716483847621
30	24.736667931198696	25.82524167359469	25.67887881747422	23.759211577732398
31	24.958902709058084	27.178829043341164	23.848266918496876	24.014001329103877
32	26.560553595979325	26.42226222089491	23.561191242981174	23.455992940144586
33	25.18086843754961	26.999104065604996	24.024763303818617	23.795264193026778
34	22.526158324791016	26.621627802485477	26.344237904213042	24.50797596851046
35	23.967455788462626	25.621840351486092	25.141318180472933	25.269385679578342
36	22.816731642089007	28.95267152569825	24.072385041931344	24.1582117902814
37	24.11677818762965	28.777789436583717	25.277457160614404	21.82797521517223
38	24.552638163576635	27.398642376889736	24.261795796910775	23.786923662622854
39	24.33443912623527	25.893849262401158	25.972411677818762	23.799299933544805
40	24.91666195830273	23.3217373055782	27.980327110221452	23.78127362589762
41	21.95442841807043	25.26481184032458	26.8247600752262	25.95599966637878
42	23.798223736073332	25.988016541155133	25.650090535112284	24.563669187659244
43	23.8079095133166	25.711971889722047	26.02406915644952	24.45604944051184
44	22.331366582454216	26.16289863026967	26.250339674826932	25.255395112449186
45	23.483974074402912	23.90907207563516	26.417688381641145	26.189265468320784
46	23.911762569313844	25.42624146104569	25.875284856018233	24.78671111362224
47	22.159174987018368	25.810443958361915	27.918983854347434	24.11139720027228
48	22.535306003298544	24.315874719852346	28.106780313119657	25.042038963729457
49	23.00237570591828	24.53219041161863	27.540969492492177	24.924464389970915
50	21.837930041783366	24.16520707384598	27.782306775470232	26.21455610890042
51	21.482515826829065	24.203950182819046	26.25464446471283	28.058889525639056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	325.0
2	527.0
3	971.5
4	1416.0
5	999.0
6	582.0
7	566.5
8	551.0
9	611.0
10	671.0
11	720.0
12	769.0
13	792.5
14	816.0
15	789.5
16	763.0
17	760.0
18	757.0
19	759.5
20	762.0
21	825.5
22	889.0
23	926.0
24	963.0
25	1207.5
26	1667.0
27	1882.0
28	2364.5
29	2847.0
30	3514.0
31	4181.0
32	5021.0
33	5861.0
34	6824.0
35	7787.0
36	8635.0
37	9483.0
38	9914.0
39	10345.0
40	12650.5
41	14956.0
42	16814.0
43	18672.0
44	21116.0
45	23560.0
46	26308.5
47	29057.0
48	31733.5
49	34410.0
50	34150.5
51	33891.0
52	31057.5
53	28224.0
54	25307.0
55	22390.0
56	21263.0
57	20136.0
58	19689.0
59	19242.0
60	18660.0
61	18078.0
62	17455.5
63	16833.0
64	14684.0
65	12535.0
66	10456.5
67	8378.0
68	7031.0
69	5684.0
70	4897.0
71	4110.0
72	3464.0
73	2818.0
74	2450.5
75	1787.0
76	1491.0
77	1096.0
78	701.0
79	573.0
80	445.0
81	385.5
82	326.0
83	223.0
84	120.0
85	90.0
86	60.0
87	50.0
88	40.0
89	24.0
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	371679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.088943139146366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20946674812916	23.492144157279434
2	9.207621483769968	5.90925684478954
3	3.233714342600269	3.112994270038263
4	1.6870240015846842	2.165392690449044
5	1.1298666224835836	1.8128112901847533
6	0.887627289041473	1.7089812964083875
7	0.7767220611213225	1.7446933037969878
8	0.6994588236246687	1.795591553557296
9	0.6112897214835631	1.7654077002807609
>10	8.460645368979849	53.26126846763268
>50	0.0789301956092184	1.6570890784069516
>100	0.014274609844220349	0.861416875393012
>500	0.003358731728051847	0.7129524717828932
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	858	0.23084435763118175	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.1740749410109261	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	601	0.16169867008897465	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	540	0.1452866586489955	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	409	0.11004119145822068	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6904936786851017E-4	0.0	0.0	0.1608915219853691	0.0
2	2.6904936786851017E-4	0.0	0.0	0.7684049946324651	0.0
3	2.6904936786851017E-4	0.0	0.0	1.0904570879710718	0.0
4	2.6904936786851017E-4	0.0	0.0	1.4751976840230414	0.0
5	2.6904936786851017E-4	0.0	0.0	2.5400950820466046	0.0
6	2.6904936786851017E-4	0.0	0.0	3.2487711170122604	0.0
7	2.6904936786851017E-4	0.0	0.0	3.9953831128473762	0.0
8	2.6904936786851017E-4	0.0	0.0	5.26798662286543	0.0
9	2.6904936786851017E-4	0.0	0.0	5.80258771682016	0.0
10	2.6904936786851017E-4	0.0	0.0	6.805065661498229	0.0
11	2.6904936786851017E-4	0.0	0.0	8.097309775370682	0.0
12	2.6904936786851017E-4	0.0	0.0	9.066694647800926	0.0
13	2.6904936786851017E-4	0.0	0.0	9.489102155354486	0.0
14	2.6904936786851017E-4	0.0	0.0	9.655912763432962	0.0
15	2.6904936786851017E-4	0.0	0.0	9.949714673145376	0.0
16	2.6904936786851017E-4	0.0	0.0	10.54108518372036	0.0
17	2.6904936786851017E-4	0.0	0.0	11.224470578106377	0.0
18	2.6904936786851017E-4	0.0	0.0	11.990454128428025	0.0
19	2.6904936786851017E-4	0.0	0.0	12.448914251275967	0.0
20	2.6904936786851017E-4	0.0	0.0	12.88719567153377	0.0
21	2.6904936786851017E-4	0.0	0.0	13.460001775725829	0.0
22	2.6904936786851017E-4	0.0	0.0	14.056484224290315	0.0
23	2.6904936786851017E-4	0.0	0.0	14.636285612046954	0.0
24	5.380987357370203E-4	0.0	0.0	15.111964894438481	0.0
25	5.380987357370203E-4	0.0	0.0	15.54728677164973	0.0
26	5.380987357370203E-4	0.0	0.0	15.926108281608592	0.0
27	8.071481036055306E-4	0.0	0.0	16.317306062489408	0.0
28	8.071481036055306E-4	0.0	0.0	16.72706824975315	0.0
29	8.071481036055306E-4	0.0	0.0	17.150282905410315	0.0
30	8.071481036055306E-4	0.0	0.0	17.660400506889008	0.0
31	8.071481036055306E-4	0.0	0.0	18.087650903064205	0.0
32	8.071481036055306E-4	0.0	0.0	18.501986929581708	0.0
33	8.071481036055306E-4	0.0	0.0	18.936232609321486	0.0
34	8.071481036055306E-4	0.0	0.0	19.35056863583899	0.0
35	8.071481036055306E-4	0.0	0.0	19.804993018168904	0.0
36	8.071481036055306E-4	0.0	0.0	20.220674291525754	0.0
37	8.071481036055306E-4	0.0	0.0	20.660300958622898	0.0
38	8.071481036055306E-4	0.0	0.0	21.103156218134465	0.0
39	8.071481036055306E-4	0.0	0.0	21.55596630425717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1615233E-6	45.000004	1
CATAAGG	30	2.1615233E-6	45.000004	2
CGATGCG	30	2.1615233E-6	45.000004	10
TAATGCG	30	2.1615233E-6	45.000004	24
GGTCGAC	20	7.0271204E-4	45.0	20
GCAACCC	25	3.8856942E-5	45.0	22
ATTACGG	25	3.8856942E-5	45.0	2
CAATTGG	20	7.0271204E-4	45.0	2
ACGGGCC	20	7.0271204E-4	45.0	5
CTAACGG	35	1.2089549E-7	45.0	2
CGGGCTA	25	3.8856942E-5	45.0	6
AGCGATA	20	7.0271204E-4	45.0	42
ATGCGAG	25	3.8856942E-5	45.0	1
AATAGCG	20	7.0271204E-4	45.0	1
TGGTCGA	20	7.0271204E-4	45.0	19
TACGTAA	20	7.0271204E-4	45.0	17
TAGCATA	125	0.0	41.4	30
ATGGGCG	115	0.0	41.086956	5
GGCGATA	110	0.0	40.90909	8
AGTACGG	55	6.002665E-11	40.90909	2
>>END_MODULE