##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638676
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33852375852545	33.0	31.0	34.0	30.0	34.0
2	32.43733912030513	34.0	31.0	34.0	31.0	34.0
3	32.52965666472515	34.0	31.0	34.0	31.0	34.0
4	36.02062861294303	37.0	35.0	37.0	35.0	37.0
5	35.990242313786645	37.0	35.0	37.0	35.0	37.0
6	36.00694405300966	37.0	35.0	37.0	35.0	37.0
7	36.15658174097665	37.0	35.0	37.0	35.0	37.0
8	36.14473535877346	37.0	36.0	37.0	35.0	37.0
9	37.723133482391695	39.0	38.0	39.0	35.0	39.0
10	37.48074297452855	39.0	37.0	39.0	35.0	39.0
11	37.420584145951935	39.0	37.0	39.0	35.0	39.0
12	37.344620746669676	39.0	37.0	39.0	35.0	39.0
13	37.31063481326995	39.0	37.0	39.0	34.0	39.0
14	38.55644019815994	40.0	38.0	41.0	34.0	41.0
15	38.619555142200426	40.0	38.0	41.0	35.0	41.0
16	38.633892615348	40.0	38.0	41.0	35.0	41.0
17	38.6387933788024	40.0	38.0	41.0	35.0	41.0
18	38.57079489443787	40.0	38.0	41.0	35.0	41.0
19	38.50903118326037	40.0	38.0	41.0	34.0	41.0
20	38.39532564242276	40.0	37.0	41.0	34.0	41.0
21	38.35087274298706	40.0	37.0	41.0	34.0	41.0
22	38.313158784735926	40.0	37.0	41.0	34.0	41.0
23	38.26655142826723	40.0	37.0	41.0	34.0	41.0
24	38.23330139225523	40.0	37.0	41.0	34.0	41.0
25	38.19859678459814	40.0	37.0	41.0	34.0	41.0
26	38.07075731669892	40.0	37.0	41.0	34.0	41.0
27	38.02226637606549	40.0	37.0	41.0	34.0	41.0
28	37.924827298974755	40.0	36.0	41.0	33.0	41.0
29	37.88110090249203	40.0	36.0	41.0	33.0	41.0
30	37.851256349072145	40.0	36.0	41.0	33.0	41.0
31	37.75201510625106	40.0	36.0	41.0	33.0	41.0
32	37.58664174009983	40.0	36.0	41.0	33.0	41.0
33	37.45690616212289	40.0	36.0	41.0	33.0	41.0
34	37.2766191308269	40.0	36.0	41.0	32.0	41.0
35	37.2440141793335	40.0	36.0	41.0	32.0	41.0
36	37.10149747289706	40.0	35.0	41.0	32.0	41.0
37	37.087239852444746	40.0	35.0	41.0	32.0	41.0
38	36.97720753558925	39.0	35.0	41.0	31.0	41.0
39	36.880484001277644	39.0	35.0	41.0	31.0	41.0
40	36.789721235806574	39.0	35.0	41.0	31.0	41.0
41	36.679718667994415	39.0	35.0	41.0	31.0	41.0
42	36.59159887016265	39.0	35.0	41.0	31.0	41.0
43	36.51210629489757	39.0	35.0	41.0	30.0	41.0
44	36.299508671063265	39.0	35.0	41.0	30.0	41.0
45	36.23614320876313	39.0	35.0	41.0	30.0	41.0
46	36.12370278513675	39.0	35.0	41.0	30.0	41.0
47	36.03912782067903	38.0	35.0	41.0	30.0	41.0
48	35.91180504669034	38.0	35.0	40.0	29.0	41.0
49	35.80636347694293	38.0	35.0	40.0	29.0	41.0
50	35.72254476448152	38.0	35.0	40.0	28.0	41.0
51	34.450561787197266	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	2.0
11	4.0
12	8.0
13	8.0
14	16.0
15	19.0
16	38.0
17	70.0
18	149.0
19	273.0
20	421.0
21	731.0
22	1078.0
23	1611.0
24	2454.0
25	3676.0
26	5100.0
27	6309.0
28	7085.0
29	8124.0
30	9713.0
31	11760.0
32	14809.0
33	19913.0
34	34815.0
35	47200.0
36	46137.0
37	68226.0
38	121372.0
39	227376.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37034740619657	20.35914923999023	24.37166889001622	11.89883446379698
2	33.56741133219347	24.12757016077009	24.908091113491036	17.396927393545397
3	32.80176490113923	24.21697386468256	26.059848812230303	16.921412421947903
4	28.826196694411564	26.146434185721713	26.253687315634217	18.773681804232506
5	26.17665295079195	30.47225823422205	23.76713701469916	19.583951800286844
6	27.23023880653101	35.315245914986626	23.016208531399332	14.438306747083027
7	81.58518560271561	6.025590440223212	8.048525386894138	4.340698570167032
8	82.53997331980536	5.11746174899323	7.923892552718437	4.418672378482986
9	75.69972881398392	7.575828745717704	10.554647426864326	6.169795013434041
10	39.67175845029405	26.61161527910866	17.496664975668413	16.219961294928883
11	31.01306452724073	25.573373666773136	24.301367203402037	19.112194602584097
12	27.645942543637148	24.088270108787555	28.19081975837514	20.074967589200156
13	24.99107528699998	23.56546981568119	30.86275357145094	20.58070132586789
14	21.740914015870334	27.032329381407788	29.469558899974324	21.757197702747558
15	21.756727981010716	25.75014561373842	32.22572947785732	20.26739692739355
16	24.567542854279793	24.450738715718142	30.698350963555853	20.28336746644621
17	24.39296294208644	24.406898020279453	28.649894469183124	22.550244568450985
18	24.756527566402998	25.39018218940433	28.301047792620988	21.552242451571686
19	25.041492086754474	26.42043853221352	26.810150999881007	21.727918381151007
20	27.257639241180193	25.585273284106496	26.697730930863223	20.45935654385009
21	26.21360439409027	26.01992246459864	26.719964426407127	21.046508714903958
22	24.575528123806123	25.02818330421059	26.62727267033676	23.769015901646533
23	23.824129918769454	26.782124269582695	27.19266106758356	22.201084744064286
24	23.34532689501406	25.403177824123656	28.45370735709499	22.79778792376729
25	23.880653101102904	25.939443473686186	26.902686182039094	23.277217243171812
26	23.010102148820373	27.48107021400522	27.435663779443725	22.07316385773068
27	23.841353049120368	26.48635614928383	27.37632226668921	22.29596853490659
28	22.288296413204815	27.28942374537324	27.75867576047949	22.663604080942452
29	23.870475796804637	26.871371399582888	26.478997175406622	22.779155628205853
30	24.316398298981017	25.952595682317796	26.40556401054682	23.325442008154372
31	23.618391798032178	27.545108944128167	25.131522086316068	23.704977171523588
32	24.16232956929648	28.01498725488354	25.721649161703276	22.101034014116706
33	23.73550908441839	27.389474475320817	25.447488241299187	23.427528198961603
34	22.309120743538195	28.577244173884726	26.183542202932315	22.930092879644764
35	22.940113610030753	26.866987330039017	25.922376917247558	24.270522142682672
36	22.16867394422211	29.457815856553243	25.075155477894896	23.29835472132975
37	23.042043226925703	28.267697549305126	25.527497510474795	23.162761713294376
38	22.593772116065107	27.7140521954794	26.22346855056398	23.468707137891514
39	23.276277799698125	26.219710776669235	25.956196882300258	24.54781454133238
40	22.696014880784624	25.81371462212452	28.191759201848825	23.298511295242033
41	20.853453081061446	26.66813846144211	27.364735797180415	25.113672660316027
42	22.1365762922045	27.203151519706392	27.013697085846346	23.646575102242764
43	22.813288741083117	25.813244900387676	27.450538301110424	23.922928057418787
44	22.97941366201329	27.09010515503949	26.172112307335798	23.75836887561142
45	22.594868133451076	25.179590277386342	26.87779092998641	25.34775065917617
46	22.459431699327983	26.511721123073357	26.755663278407205	24.27318389919145
47	22.484326951380666	26.46365293200308	27.67381270002317	23.378207416593078
48	22.384902517082214	25.390808485053455	27.533365900707086	24.690923097157246
49	21.914241336765432	25.957292899686223	27.573918543987872	24.554547219560465
50	20.870206489675518	25.697380205299712	28.20882575828746	25.223587546737313
51	21.513725269150555	25.109914886421286	27.225228441338018	26.151131403090144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	556.0
2	861.0
3	2616.0
4	4371.0
5	2973.5
6	1576.0
7	1574.0
8	1572.0
9	1542.0
10	1512.0
11	1532.0
12	1552.0
13	1570.5
14	1589.0
15	1592.5
16	1596.0
17	1512.0
18	1428.0
19	1590.0
20	1752.0
21	1780.5
22	1809.0
23	2030.5
24	2252.0
25	2728.5
26	4048.5
27	4892.0
28	5956.5
29	7021.0
30	7935.5
31	8850.0
32	10317.5
33	11785.0
34	13090.5
35	14396.0
36	15958.0
37	17520.0
38	19027.5
39	20535.0
40	23815.5
41	27096.0
42	30150.0
43	33204.0
44	36901.5
45	40599.0
46	45431.5
47	50264.0
48	53998.5
49	57733.0
50	56868.0
51	56003.0
52	52344.0
53	48685.0
54	44594.0
55	40503.0
56	37711.0
57	34919.0
58	33576.0
59	32233.0
60	31113.0
61	29993.0
62	27345.5
63	24698.0
64	21490.5
65	18283.0
66	15135.5
67	11988.0
68	9796.5
69	7605.0
70	6463.0
71	5321.0
72	4536.5
73	3752.0
74	3112.0
75	1914.0
76	1356.0
77	1122.0
78	888.0
79	644.5
80	401.0
81	312.0
82	223.0
83	147.5
84	72.0
85	48.5
86	25.0
87	21.0
88	17.0
89	11.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	3.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	638676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.31415620636678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60741145352384	23.855404906962708
2	8.217431606055905	5.4751360027856375
3	3.2184779707714744	3.216626335950938
4	2.078934321950939	2.7703177097700316
5	1.5782573729250393	2.6289156327737415
6	1.302708882023058	2.603918831228255
7	1.2064283875630364	2.8133800628549133
8	1.1137330447061917	2.968246129882764
9	1.0545009588313803	3.161682869604493
>10	8.540562626767546	47.05106314242215
>50	0.053268967409955896	1.2197700279893762
>100	0.02639878030685521	1.5596158294171656
>500	0.0014142203735815293	0.30670926541654664
>1k	4.714067911938431E-4	0.3692132529412704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2351	0.3681052677727048	No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	779	0.12197107766692344	Illumina PCR Primer Index 8 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.131478245620628E-4	0.0	0.0	0.10787942556163062	0.0
2	3.131478245620628E-4	0.0	0.0	0.41930493708860206	0.0
3	3.131478245620628E-4	0.0	0.0	0.6569841359312076	0.0
4	3.131478245620628E-4	0.0	0.0	0.9281701520019541	0.0
5	3.131478245620628E-4	0.0	0.0	1.707125365600085	0.0
6	3.131478245620628E-4	0.0	0.0	2.4766861444613544	0.0
7	3.131478245620628E-4	0.0	0.0	3.1317913934451895	0.0
8	3.131478245620628E-4	0.0	0.0	4.240491266307173	0.0
9	3.131478245620628E-4	0.0	0.0	4.793666898396057	0.0
10	3.131478245620628E-4	0.0	0.0	5.695376059222516	0.0
11	4.697217368430942E-4	0.0	0.0	6.682418002242138	0.0
12	4.697217368430942E-4	0.0	0.0	7.454640537612185	0.0
13	4.697217368430942E-4	0.0	0.0	7.814760535858557	0.0
14	4.697217368430942E-4	0.0	0.0	7.979163143753641	0.0
15	4.697217368430942E-4	0.0	0.0	8.226393351245388	0.0
16	4.697217368430942E-4	0.0	0.0	8.698307122860417	0.0
17	4.697217368430942E-4	0.0	0.0	9.2605640418616	0.0
18	4.697217368430942E-4	0.0	0.0	9.845993899880378	0.0
19	4.697217368430942E-4	0.0	0.0	10.232574889302244	0.0
20	4.697217368430942E-4	0.0	0.0	10.61445866135568	0.0
21	4.697217368430942E-4	0.0	0.0	11.067113841760142	0.0
22	4.697217368430942E-4	0.0	0.0	11.561261108919076	0.0
23	4.697217368430942E-4	0.0	0.0	12.060262167358724	0.0
24	4.697217368430942E-4	0.0	0.0	12.473773869692927	0.0
25	4.697217368430942E-4	0.0	0.0	12.843914598325286	0.0
26	4.697217368430942E-4	0.0	0.0	13.186654892308463	0.0
27	6.262956491241256E-4	0.0	0.0	13.53064777758989	0.0
28	6.262956491241256E-4	0.0	0.0	13.894368975818725	0.0
29	6.262956491241256E-4	0.0	0.0	14.289091808679204	0.0
30	6.262956491241256E-4	0.0	0.0	14.731099963048557	0.0
31	6.262956491241256E-4	0.0	0.0	15.137722413242395	0.0
32	6.262956491241256E-4	0.0	0.0	15.549981524278351	0.0
33	6.262956491241256E-4	0.0	0.0	15.9400071397704	0.0
34	7.828695614051569E-4	0.0	0.0	16.328153868315077	0.0
35	7.828695614051569E-4	0.0	0.0	16.711759953403604	0.0
36	7.828695614051569E-4	0.0	0.0	17.12715054268518	0.0
37	7.828695614051569E-4	0.0	0.0	17.530015218984275	0.0
38	7.828695614051569E-4	0.0	0.0	17.9350719300553	0.0
39	7.828695614051569E-4	0.0	0.0	18.348427058477224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCTA	30	2.1639808E-6	45.000004	10
TCGATTG	20	7.030929E-4	45.0	12
TCTACGG	20	7.030929E-4	45.0	2
TACACCG	25	3.8888524E-5	45.0	1
TACCGGG	70	0.0	41.785713	3
TTTACGG	45	1.925946E-8	40.000004	2
TCGTAAG	45	1.925946E-8	40.000004	1
TATAGCG	45	1.925946E-8	40.000004	1
CGTAAGG	145	0.0	38.793102	2
TACGGGT	70	0.0	38.571426	4
CGTTTTT	1460	0.0	38.373287	1
ACGGGCG	165	0.0	38.181816	5
CGTTGCG	30	1.1393537E-4	37.500004	28
CGATTGC	30	1.1393537E-4	37.500004	10
ATCGTAG	30	1.1393537E-4	37.500004	1
ATAAGCG	30	1.1393537E-4	37.500004	1
AATAGCG	30	1.1393537E-4	37.500004	1
ATACCGG	30	1.1393537E-4	37.500004	2
TAGGGCG	275	0.0	36.818184	5
ACGGGTA	55	2.744855E-9	36.81818	5
>>END_MODULE