FastQCFastQC Report
Sat 18 Jun 2016
SRR3550416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684778
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.9040886243424877No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG17990.2627128792104887Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT13690.19991880580275653No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC11900.17377894733767732Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT10440.15245816892481942Illumina PCR Primer Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC10000.14603272885519103Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT9800.1431120742780872No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT7890.11521982306674572Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC7420.10835628481055173Illumina PCR Primer Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA351.210974E-745.09
CGACAGG850.042.3529432
CGTTTTT34400.042.0566861
CATACGG451.9263098E-840.02
GCGATAT403.4554978E-739.3759
GTTTACG356.2445906E-638.5714261
CAACCCG356.2445906E-638.57142623
CGTAAGG1250.037.8000032
GCGATCA900.037.59
ATTTACG301.13944E-437.4999961
TTAAGCG301.13944E-437.4999961
ACCGTAC301.13944E-437.49999630
ACAACGA1750.037.28571313
ACGGGCT1700.037.0588265
ACGCGCA850.037.05882617
TGATTCG552.746674E-936.81818415
CTACGAA552.746674E-936.81818411
TACGAAT552.746674E-936.81818412
GATACCG250.00210651536.01
GTATTAG504.873982E-836.01
Produced by FastQC (version 0.11.5)