##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684778
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25881088469548	33.0	31.0	34.0	30.0	34.0
2	32.39531789864745	34.0	31.0	34.0	30.0	34.0
3	32.44223529377404	34.0	31.0	34.0	30.0	34.0
4	35.95976798320039	37.0	35.0	37.0	35.0	37.0
5	35.937246815756346	37.0	35.0	37.0	35.0	37.0
6	35.959474457415396	37.0	35.0	37.0	35.0	37.0
7	36.1256217343431	37.0	35.0	37.0	35.0	37.0
8	36.12296101802336	37.0	36.0	37.0	35.0	37.0
9	37.717337589700605	39.0	38.0	39.0	35.0	39.0
10	37.45824632216572	39.0	37.0	39.0	35.0	39.0
11	37.38944884327476	39.0	37.0	39.0	35.0	39.0
12	37.30168609388736	39.0	37.0	39.0	34.0	39.0
13	37.26037343489423	39.0	37.0	39.0	34.0	39.0
14	38.49983206236182	40.0	38.0	41.0	34.0	41.0
15	38.562237688710795	40.0	38.0	41.0	34.0	41.0
16	38.574882662702365	40.0	38.0	41.0	34.0	41.0
17	38.56719549985543	40.0	38.0	41.0	34.0	41.0
18	38.45949636232472	40.0	38.0	41.0	34.0	41.0
19	38.35088452023868	40.0	37.0	41.0	34.0	41.0
20	38.181247061091334	40.0	36.0	41.0	34.0	41.0
21	38.12614161085783	40.0	36.0	41.0	34.0	41.0
22	38.100956806439456	40.0	36.0	41.0	34.0	41.0
23	38.063236552576164	40.0	36.0	41.0	34.0	41.0
24	38.0302200129093	40.0	36.0	41.0	34.0	41.0
25	37.98440370455827	40.0	36.0	41.0	34.0	41.0
26	37.86026274208576	40.0	36.0	41.0	33.0	41.0
27	37.78760853882572	40.0	36.0	41.0	33.0	41.0
28	37.69306695016487	40.0	35.0	41.0	33.0	41.0
29	37.62373937246816	40.0	35.0	41.0	33.0	41.0
30	37.547625653861544	40.0	35.0	41.0	33.0	41.0
31	37.40716699426675	40.0	35.0	41.0	33.0	41.0
32	37.18378657024612	40.0	35.0	41.0	32.0	41.0
33	36.9683269614386	40.0	35.0	41.0	31.0	41.0
34	36.710973483377096	39.0	35.0	41.0	31.0	41.0
35	36.602275481981025	39.0	35.0	41.0	30.0	41.0
36	36.43338278975084	39.0	35.0	41.0	30.0	41.0
37	36.36777612598536	39.0	35.0	41.0	30.0	41.0
38	36.27987610583284	39.0	35.0	41.0	30.0	41.0
39	36.16732722137686	39.0	35.0	41.0	29.0	41.0
40	36.08373516672557	39.0	35.0	41.0	29.0	41.0
41	35.92154537674984	39.0	35.0	41.0	28.0	41.0
42	35.83467342700846	39.0	35.0	41.0	28.0	41.0
43	35.72792204188803	39.0	35.0	41.0	28.0	41.0
44	35.54393978778524	38.0	35.0	41.0	27.0	41.0
45	35.46749603521141	38.0	35.0	40.0	26.0	41.0
46	35.345662389854816	38.0	35.0	40.0	26.0	41.0
47	35.245873845246194	38.0	34.0	40.0	26.0	41.0
48	35.10698649781389	38.0	34.0	40.0	26.0	41.0
49	34.999693331269405	38.0	34.0	40.0	25.0	41.0
50	34.91918256719696	37.0	34.0	40.0	25.0	41.0
51	33.63361848657521	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	2.0
11	4.0
12	4.0
13	10.0
14	12.0
15	24.0
16	51.0
17	80.0
18	184.0
19	356.0
20	591.0
21	978.0
22	1565.0
23	2403.0
24	3588.0
25	5874.0
26	8386.0
27	10093.0
28	10318.0
29	10683.0
30	11914.0
31	13868.0
32	16939.0
33	22313.0
34	38458.0
35	51246.0
36	51037.0
37	73211.0
38	127933.0
39	222497.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.175616039066675	20.748038050287832	24.379725984187576	15.696619926457917
2	35.19520194866073	23.28448051777364	24.221864604295114	17.298452929270507
3	31.313652015689758	22.900706506342203	28.917108902447215	16.868532575520824
4	27.149236686926276	25.260887470099796	29.13046271930465	18.45941312366928
5	24.7518173773106	29.27386685904045	27.195967160159935	18.778348603489015
6	26.244709964397224	33.043409688979494	25.960384241316163	14.75149610530712
7	78.60971000820703	5.547053205564431	11.098779458452228	4.744457327776301
8	79.77797183904856	4.619161246418548	11.10301440758903	4.499852506943856
9	73.02249780220743	6.944440387979754	13.584986667211856	6.448075142600959
10	38.18726653017474	26.664992158042462	19.914045135795835	15.233696175986964
11	28.485582188680127	24.172067443755495	28.426584966222627	18.91576540134175
12	25.700445983953923	22.225889266302364	31.30050907009279	20.773155679650923
13	23.418392530133854	22.184269938578634	33.919898127568345	20.47743940371916
14	20.833175131210407	25.369827885825767	32.3628971725143	21.434099810449517
15	20.629897572643983	24.894199287944414	34.2925736516068	20.18332948780481
16	23.272067735820954	24.3789958205433	32.701839136187196	19.647097307448547
17	22.311172379953796	24.356944878486168	30.89906509846987	22.432817643090168
18	23.374582711477295	24.132930672422304	30.888842807450008	21.603643808650393
19	23.229718244452947	26.030333918437798	29.381346947477873	21.358600889631386
20	25.070314758943773	25.06374328614529	29.009547619812555	20.856394335098383
21	23.80143637792102	25.860789920236925	29.828499163232465	20.509274538609592
22	23.206353007836118	23.82509367999556	29.463125275636777	23.505428036531548
23	22.548913662530047	25.642470990598415	29.378426292900766	22.430189053970775
24	22.698597209606618	24.22829004436474	30.68673351071442	22.38637923531422
25	23.20255615688588	25.398888398867953	28.47638212676225	22.922173317483914
26	22.132866418021607	25.923437960915802	29.642891564857514	22.300804056205077
27	23.401160668128938	24.875945196837517	29.551036978407602	22.171857156625943
28	21.74106060650313	25.627575652255185	30.061129300298784	22.570234440942905
29	23.712356413319352	24.129133821472067	29.395074023990258	22.76343574121832
30	23.95111992499759	23.760693246570423	29.901661560388916	22.386525268043073
31	23.333693547397843	25.659994918061034	28.517125258112845	22.48918627642827
32	23.97492325980099	25.987546328883234	28.437391388157913	21.60013902315787
33	22.79629310521074	25.702490442157895	28.104290733639225	23.39692571899214
34	22.083799421126262	26.2750847719991	28.92601689890738	22.715098907967253
35	23.269001048514994	25.892186956940787	28.107211388216324	22.73160060632789
36	22.542926320646984	27.06336944235942	27.415308318900433	22.978395918093163
37	22.710425860643888	27.567328389638686	28.040620463858364	21.681625285859067
38	22.830318731033998	26.750713369880458	27.135217544956173	23.283750354129367
39	22.483052901816354	25.430869566487242	27.22006256042104	24.866014971275362
40	23.80990627619462	24.8319893454521	28.566192255008193	22.791912123345085
41	22.141190283566353	25.358583365703925	28.1669387743181	24.333287576411625
42	23.334277678313263	25.26964943383111	28.290336430200735	23.105736457654892
43	22.80417887256892	24.35928140214785	29.27255256448075	23.56398716080248
44	22.746788010128828	25.077470362657678	28.165478447029546	24.010263180183944
45	22.770007214016804	23.733677191732212	28.711786885676815	24.784528708574165
46	23.05345674072473	24.639956307007527	27.710294431187915	24.596292521079825
47	22.038383242452298	25.106092777513293	29.35038800896057	23.505135971073837
48	22.269991150416633	24.12694333053924	29.295771768368727	24.307293750675402
49	22.42887475941108	23.69629281314528	29.338267292465588	24.53656513497805
50	21.240168346529824	23.42700846113631	30.1080058062613	25.22481738607257
51	21.580132539304707	23.53054566589464	28.664180216070022	26.225141578730625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	722.0
2	1043.0
3	5554.0
4	10065.0
5	6735.5
6	3406.0
7	3350.0
8	3294.0
9	3342.0
10	3390.0
11	3428.0
12	3466.0
13	3423.5
14	3381.0
15	3148.5
16	2916.0
17	2794.0
18	2672.0
19	2505.5
20	2339.0
21	2467.5
22	2596.0
23	2769.5
24	2943.0
25	3464.0
26	4570.5
27	5156.0
28	5842.0
29	6528.0
30	7563.5
31	8599.0
32	9996.0
33	11393.0
34	13075.5
35	14758.0
36	16249.5
37	17741.0
38	19689.5
39	21638.0
40	24510.5
41	27383.0
42	30152.0
43	32921.0
44	37545.0
45	42169.0
46	48677.5
47	55186.0
48	59879.5
49	64573.0
50	63558.5
51	62544.0
52	55866.5
53	49189.0
54	44452.5
55	39716.0
56	37238.0
57	34760.0
58	32760.5
59	30761.0
60	29983.5
61	29206.0
62	26881.5
63	24557.0
64	21589.0
65	18621.0
66	15765.5
67	12910.0
68	10837.5
69	8765.0
70	7923.5
71	7082.0
72	5983.5
73	4885.0
74	4119.5
75	2734.0
76	2114.0
77	1697.5
78	1281.0
79	866.0
80	451.0
81	387.5
82	324.0
83	259.0
84	194.0
85	125.5
86	57.0
87	44.0
88	31.0
89	27.0
90	23.0
91	15.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.5953365462435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65306158278199	25.86110178154079
2	8.550970903695362	6.087493742283447
3	3.406707899527133	3.637887425952438
4	2.128982527015147	3.0312739820070442
5	1.6158650569119846	2.8758630257048496
6	1.3795201399155845	2.9462690191569694
7	1.1662921455032447	2.9060193001299877
8	1.074677813445503	3.0602814758699015
9	0.9325559121816658	2.9875177388065945
>10	7.010534874230337	41.64001943954499
>50	0.056911724511190205	1.3694647406561717
>100	0.019795382301420226	1.3019383341812265
>500	0.0020620189897312736	0.593057940026176
>1k	0.0016496151917850185	0.7929948000052978
>5k	4.1240379794625463E-4	0.9088172541341721
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6191	0.9040886243424877	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1799	0.2627128792104887	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1369	0.19991880580275653	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1190	0.17377894733767732	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	1044	0.15245816892481942	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	1000	0.14603272885519103	Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	980	0.1431120742780872	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	789	0.11521982306674572	Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	742	0.10835628481055173	Illumina PCR Primer Index 11 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17202655459141503	0.0
2	0.0	0.0	0.0	0.5679212825178379	0.0
3	0.0	0.0	0.0	1.0582991860135693	0.0
4	0.0	0.0	0.0	1.4733242014200223	0.0
5	0.0	0.0	0.0	2.5187725072943348	0.0
6	0.0	0.0	0.0	3.6277450502206556	0.0
7	0.0	0.0	0.0	4.580900671458488	0.0
8	0.0	0.0	0.0	6.013043643341345	0.0
9	0.0	0.0	0.0	6.743499353075011	0.0
10	0.0	0.0	0.0	7.833487641250157	0.0
11	0.0	0.0	0.0	8.99488593383549	0.0
12	0.0	0.0	0.0	9.887584005327273	0.0
13	0.0	0.0	0.0	10.333860024708738	0.0
14	0.0	0.0	0.0	10.566636194503912	0.0
15	0.0	0.0	0.0	10.854758768535204	0.0
16	0.0	0.0	0.0	11.360470108560731	0.0
17	0.0	0.0	0.0	11.959496362324725	0.0
18	0.0	0.0	0.0	12.579551329043865	0.0
19	0.0	0.0	0.0	12.962887242288742	0.0
20	0.0	0.0	0.0	13.366521704844489	0.0
21	1.4603272885519103E-4	0.0	0.0	13.858359935628773	0.0
22	1.4603272885519103E-4	0.0	0.0	14.361442686534906	0.0
23	1.4603272885519103E-4	0.0	0.0	14.876208055749455	0.0
24	1.4603272885519103E-4	0.0	0.0	15.30758873678769	0.0
25	2.9206545771038206E-4	0.0	0.0	15.69778818828876	0.0
26	2.9206545771038206E-4	0.0	0.0	16.0744065960063	0.0
27	2.9206545771038206E-4	0.0	0.0	16.436275698109462	0.0
28	2.9206545771038206E-4	0.0	0.0	16.820779873185177	0.0
29	2.9206545771038206E-4	0.0	0.0	17.245297015967218	0.0
30	2.9206545771038206E-4	0.0	0.0	17.69288732990838	0.0
31	2.9206545771038206E-4	0.0	0.0	18.107912345314833	0.0
32	2.9206545771038206E-4	0.0	0.0	18.525419917111822	0.0
33	2.9206545771038206E-4	0.0	0.0	18.915911434070605	0.0
34	4.380981865655731E-4	0.0	0.0	19.321590354830324	0.0
35	4.380981865655731E-4	0.0	0.0	19.71982160641843	0.0
36	4.380981865655731E-4	0.0	0.0	20.135722818198015	0.0
37	4.380981865655731E-4	0.0	0.0	20.543154131703997	0.0
38	4.380981865655731E-4	0.0	0.0	20.953506099787084	0.0
39	5.841309154207641E-4	0.0	0.0	21.38328042080791	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	35	1.210974E-7	45.0	9
CGACAGG	85	0.0	42.352943	2
CGTTTTT	3440	0.0	42.056686	1
CATACGG	45	1.9263098E-8	40.0	2
GCGATAT	40	3.4554978E-7	39.375	9
GTTTACG	35	6.2445906E-6	38.571426	1
CAACCCG	35	6.2445906E-6	38.571426	23
CGTAAGG	125	0.0	37.800003	2
GCGATCA	90	0.0	37.5	9
ATTTACG	30	1.13944E-4	37.499996	1
TTAAGCG	30	1.13944E-4	37.499996	1
ACCGTAC	30	1.13944E-4	37.499996	30
ACAACGA	175	0.0	37.285713	13
ACGGGCT	170	0.0	37.058826	5
ACGCGCA	85	0.0	37.058826	17
TGATTCG	55	2.746674E-9	36.818184	15
CTACGAA	55	2.746674E-9	36.818184	11
TACGAAT	55	2.746674E-9	36.818184	12
GATACCG	25	0.002106515	36.0	1
GTATTAG	50	4.873982E-8	36.0	1
>>END_MODULE